Introduction
Familial hypercholesterolemia (FH) is a genetic disorder characterized by elevated levels of low-density lipoprotein cholesterol (LDL-C) in the bloodstream. This condition is inherited in an autosomal co-dominant pattern. The observed phenomenon can be attributed to a genetic mutation in the lipid metabolizing genes, which are LDL receptor (LDLR), apolipoprotein B (APOB), and proprotein convertase subtilisin/kexin type 9 (PCSK9). A total of 64 additional gene loci have been implicated in polygenic etiology.1 According to the findings of epidemiological research, the heterozygous variant of FH, known as HeFH, demonstrates a prevalence of approximately 1 in every 300 individuals.2,3
Conversely, the homozygous form of FH, known as HoFH, demonstrates a prevalence of approximately 1 in every 500 individuals. In instances of homozygous familial FH, individuals have symptoms characterized by plasma LDL levels that are twice as detrimental compared to heterozygote patients. The disorder arises from genetic anomalies that affect the gene responsible for regulating the cellular receptor for LDL. As a result, there is an interruption in the process of attaching or absorbing this lipoprotein. Individuals diagnosed with HoFH frequently demonstrate a restricted reaction to existing treatments, owing to decreased functionality of the LDL receptor.4,5 Generally, LDL readings of 150 mg/dL are typically considered normal for adults in good health. Nevertheless, current guidelines recommend that those at high risk of cardiovascular disease (CVD) reduce their LDL levels to 70 mg/dL. It is advisable to strive for a minimum reduction of 30% in LDL-C levels. Individuals at high risk should aim for a decrease of at least 50%.2,6,7 This vascular intima disease is characterized by the presence of intimal plaques, which have the potential to impact both the aorta and coronary arteries. These plaques form when tiny cholesterol crystals accumulate in the intima and smooth muscle.8 Compared to those with normal LDL cholesterol levels, individuals with this disorder are three to ten times more likely to develop early asymptomatic atherosclerotic cardiovascular disease.9,10 If untreated, young adult FH patients (20–40 years old) have a 100-fold increased risk of disease mortality. Commonly, the first-line therapies consist of cholesterol-lowering drugs including statins, ezetimibe, and PCSK9 inhibitors. Nevertheless, their efficacy is constrained by the diminished functionality of LDLR. Despite the recent noteworthy advancements in therapeutic interventions for atherosclerosis, the primary hurdle that persists is identifying and elucidating biomarkers for early detection and the precise molecular mechanisms underlying the disease. When a condition occurs, multiple underlying physiological processes occur throughout the body. Examining the underlying genes responsible prior to treatment is crucial in this instance. This is why many treatments frequently fail, due to insufficient understanding of the genes involved. Within the current medical context, it is evident that a standardized approach to patient treatment is no longer adequate. Hence, it is imperative to provide customized treatment for each individual, also known as individualized treatment, personalized. The investigation of multi-dimensional data necessitates the application of unconventional methodologies and technologies. Various biomarker approaches are currently being investigated as potential diagnostic tests for atherosclerosis and related disorders. Recently, data from gene expression microarrays in atherosclerosis have been employed to identify biomarkers, repurpose drugs already in use, and uncover new therapeutic targets.11,12 A comparative analysis of the changes in gene expression observed in individuals diagnosed with atherosclerosis in contrast to those observed in individuals without the disease shows promise in elucidating the underlying pathophysiological mechanisms that contribute to dysfunction and identifying a distinct gene expression pattern associated with the disease. Pathway enrichment analysis is a potent method for data analysis in genomics, primarily gene expression research. The software analyzes a wide range of omics data types, including protein-protein interactions and genomics, proteomics, and metabolomics. This primarily entails analyzing biological aspects that are similar to the collection of features that the researcher thought was important, which are typically features with significant expression changes or differentially expressed genes (DEGs).13
Gene set enrichment analysis is a widely used method for interpreting the findings of gene expression research. This method is predicated on the functional annotation of genes expressed at different levels. This strategy’s applicability lies in determining whether the differentially expressed genes are associated with a specific biological activity or molecular function. Almost all transcriptome analyzes include enrichment analysis, which provides a valuable overview of the processes or functions connected to the genes of interest.14 With a user-friendly online interface, bench researchers can use networkanalyst.ca, a full-featured web-based application, to perform a variety of simple and complex meta-analyzes of gene expression data. In order to enhance our comprehension of fundamental biological mechanisms, we conducted comprehensive research investigations as mentioned in Fig. 1, that yielded substantial datasets of genes. The genes, which exhibit varying degrees of activity under different circumstances, are examined to derive a functional profile of the group of genes.15,16 In this study, we utilized networkanalyst.ca, a platform that employs limma to analyze the differential expression of genes in individuals with FH and atherosclerosis. Through analysis, we can identify the genes that have a key role in our specific ailment, offering a fresh perspective for diagnosis and study.
Methods
Data retrieval
The GEO (Gene Expression Omnibus) database provides web-based technical assistance for retrieving, identifying, and analyzing gene expression patterns submitted to the database. Three atherosclerosis and FH datasets were chosen from the pool of hypercholesterolemia datasets examined within the GEO collection. The datasets that have been obtained for analysis include GSE6054 (Monocytes of patients with FH show alterations in cholesterol metabolism), GSE6088 (Imprints of atherosclerosis are present in circulating T cells of patients with FH), and GSE75545 (Urine-sample-derived human induced pluripotent stem cells as a model to study PCSK9-mediated autosomal dominant hypercholesterolemia)
To analyze the data in networkanalyst.ca, a dataset must include a gene name file containing the matching analysis code and gene code for each gene. The gene code should include the official gene symbol, Ensemble gene ID, GenBank ID, Ref Seq ID, Entrez ID, Ensemble ID, and UniProt Accession ID. There are downloadable files with Affymetrix human genome and Agilent Human 18 cDNA microarray data. Additionally, there is another file that includes the aforementioned code and the accompanying empirical data for the research investigation. When selecting datasets, it is crucial to verify that they include the required data and genetic code. Alternatively, the software will reject them. Furthermore, the dataset must include information regarding the sample type, specifically differentiating between patient samples and control samples. Upon thorough examination, we have determined that there are only three datasets that satisfy the criteria for investigating FH.
Data processing
All of the retrieved raw data were converted to an Excel file, and the entries in the Excel file were inspected for unnecessary information in the series matrix file, such as RefSeq, control type, UniGene ID, ensemble ID, accession string, chromosome number, gene ontology (GO) ID, and sequence data, and eliminated. Only the ID and Gene symbol are necessary. These unnecessary additions render the final document incomprehensible. The platform files containing the gene expression datasets were also examined for undesired elements and deleted; the two files were then integrated and processed jointly using the ID data.
Normalization and differential expression
Using Network analysis, we may rapidly and easily browse sizable complex gene expression data sets to identify essential characteristics, patterns, functions, and relationships that inspire the development of novel biological ideas.17 The purpose is to standardize the data by eliminating duplicate data, as the same information will be recorded in multiple databases. The main objective of the normalization is to get the database organized. The first step is to eliminate any duplicate data from the data set. This modifies the database to eliminate duplicated entries, missing data, and errored information. It is easier to evaluate and clean up the data if we try to eliminate them from the database. This will occur in a database through the log2 normalization procedure. Based on the well-established linear model such as limma, Network-Analyst can conduct extremely complicated Differential expressions for the entered microarray datasets.18
Interpretation of DEGs
The findings of gene expression analysis revealed information concerning the genes as well as the expression levels of each gene in question. These data were manually input into an Excel spreadsheet, which was then used to categorize the data based on whether regulation increased or decreased. The relative depiction of mean expression levels, log fold changes, and corrected p-values can benefit from the use of both MA plots and volcano plots. Both plots can be helpful. To further comprehend the findings, we utilized the Volcano Plot in conjunction with the MA plot. The MA plot and the volcano were both created using the ORIGIN software with DEG data. The x-axis of the volcano plot represented the log2 fold change, while the y-axis represented the log2 of the p-value. The log2 fold change was plotted along the y-axis in the MA plot, while the log2Exp was plotted along the x-axis. Both of these measurements are expressed as percentages.19
Network construction
The three major processes of network analysis include data processing to identify significant genes, network construction to map, create, and improve networks, and network analysis and visualization. The direct or indirect links between expressed proteins can be researched more effectively with the assistance of the STRING website.20,21 The DEGs that resulted from the events that were described. The PPI networks exhibited a maximum enrichment p-value at 1.0 × 10−16 and a minimal average local clustering coefficient value of higher than 0.4. The network was constructed using nodes with a confidence score higher than 0.4 and a maximum number of extra interactions of 10.
The Molecular Complex Detection (MCODE) Cytoscape plugin was utilized to find clusters inside the PPI networks.22 The parameters for these calculations were as follows: a k-core value of 2, a node score of 0.2, a degree cutoff of 2, and a network depth of 100. The data collected for this study was presented using an illustration of a network structure. We then utilized the Cytoscape plugin to focus our search for hub genes on those with high MCODE scores (more than 5). In addition, well-established FH candidate genes (LDLR, LDLRAP1, PCSK9, APOB, ABCG5, APOA1, and ABCG8) were utilized to construct subnetworks with the primary genes.23
GO annotation
The Enrichr web server, located at https://maayanlab.cloud/Enrichr , was provided with the overexpressed protein set.24,25 The Enrichr program offers a GO analysis feature that allows for the examination of the biological implications of protein-protein interaction across multiple dimensions, including Biological Processes (BPs), Cellular Components (CC), Molecular Functions (MFs), and Reactome. This analysis focuses on subnetworks with a p-value below 0.05, which yield valuable scientific insights. A thorough examination of the GO annotation was performed employing the Enrichment Analysis Visualization Appyter to determine the comparative importance of discrete terms. The Appyter is a tool that expedites the generation of scatterplots, bar charts, hexagonal canvases, Manhattan plots, and volcano plots, thereby facilitating their construction. Following that, the results will be examined at how each gene set is expressed in different disease-related biological pathways, the pathway’s activity score will be estimated using samples, and the critical pathways will be found in a pathway network. The biological pathway serves as the foundation for explaining human activity and provides information on the pathway’s gene similarity (Fig. 1).
Results
Interpretation of DEGs
The obtained datasets GSE6054 (Monocytes of patients with FH show alterations in cholesterol metabolism), GSE6088 (Imprints of atherosclerosis are present in circulating T cells of patients with FH and GSE75545 (Urine-sample-derived human induced pluripotent stem cells as a model to study PCSK9-mediated autosomal dominant hypercholesterolemia) were analyzed using networkanlayst.ca.26 The differentially expressed genes are HLA-DRB5, WARS2, NLRP2, HERC2P7, PEX6, OSM, HSD17B3, MYO18B, RGPD4-AS1, CYS1, FAM184B, MYOM2, LINC00958, CLDN8, LINC00644, ZNHIT3, LY96, CD52, CLIC3, NDUFA4, LAIR2, EVI2A, RSL24D1, RPL34, KLRF1, GZMA, AKR1C3, COMMD6, COPS2, COX7B, TCN1, RPS7, RPL9, YOD1, RNF182, and XK and they play vital roles in various cellular functions (Table 1). HLA-DRB5 is an Human Leukocyte Antigen (HLA) system component that regulates the immunological response. HLA genes produce proteins that aid the immune system in differentiating between the body’s proteins and those produced by external invaders such as viruses and bacteria. WARS2 is a gene that encodes tryptophanyl-tRNAsynthetase, a component of mitochondrial protein synthesis. NLRP2 is a member of the NLR family that regulates inflammation and immunological reactions. It is understood to play a role in the synthesis of inflammasomes. Participates in peroxisome biogenesis (PEX6), and the rare congenital condition Zellweger syndrome is linked to mutations in this gene. The gene OSM encodes the oncostatin M, a protein involved in a variety of biological processes such as liver regeneration, inflammation, and tumor cell growth suppression. HSD17B3 encodes an enzyme required for producing androgens, the male sex hormones. Myosin, a protein usually involved in several cellular functions, including motility, polarity, and division, is encoded by the gene MYO18B. Associated with cystinuria, a condition in which specific amino acids, especially cystine, move abnormally in the kidneys. CLDN8 is a member of the claudin family and contributes to the cells. ZNHIT3 is a member of the HIT zinc finger protein family; although its precise function is still unknown, HIT proteins are typically implicated in nucleic acid binding. LY96 generates a protein that aids in the immune system’s identification of bacterial compounds, triggering an inflammatory reaction. CD52 codes for a protein targeted for various leukemia and multiple sclerosis therapies. CLIC3 is A member of the family of chloride intracellular channels, which is involved in the transport of chloride ions into cells. NDUFA4 encodes a Complex I subunit, which is part of the mitochondrial respiratory chain. LAIR2 is an immune cell receptor that aids in controlling immunological reactions. EVI2A may have a role in immune cell development, while its exact function is uncertain. The ribosomal proteins, known as RSL24D1, RPL34, RPS7, and RPL9, are essential parts of ribosomes, the organelles responsible for protein synthesis in cells. KLRF1 is an immune system protein identified in natural killer cells. Granzyme A, an enzyme secreted by cytotoxic T cells and natural killer cells to target and eliminate infected or damaged cells, is encoded by the gene GZMA. AKR1C3 mediates prostaglandin and steroid metabolism. The genes COMMD6, COPS2, COX7B, YOD1, RNF182, and XK participate in a variety of processes, including membrane transport, ubiquitin-protein ligase activity, COP9 signalosome complex, mitochondrial respiratory chain, and copper metabolism.
Table 1Interpretation of differentially expressed genes obtained from datasets GSE6054 (Monocytes of patients with familial hypercholesterolemia show alterations in cholesterol metabolism), GSE6088 (Imprints of atherosclerosis are present in circulating T cells of patients with familial hypercholesterolemia) and GSE75545 (Urine-sample-derived human induced pluripotent stem cells as a model to study PCSK9-mediated autosomal dominant hypercholesterolemia) analyzed using networkanlayst.ca
EntrezID | adj.P.Val | p-Value | t | B | logFC | Symbols | Name |
---|
Monocyte (GSE6054) | | | | | | | |
84700 | 0.013008 | 0.000905 | 4.7528 | 1.5037 | 2.4492 | MYO18B | Myosin XVIIIB |
1.02 × 108 | 0.046545 | 0.001005 | 3.7765 | −0.72929 | 2.1865 | LINC00644 | Long intergenic non-protein coding RNA 644 |
192668 | 0.025636 | 0.000317 | 4.2456 | 0.34062 | 2.0798 | CYS1 | Cystin 1 |
9073 | 0.036811 | 0.000647 | 3.9562 | −0.32126 | 2.0366 | CLDN8 | Claudin 8 |
729121 | 0.022197 | 0.000254 | 4.3352 | 0.54608 | 1.7591 | RGPD4-AS1 | RGPD4 antisense RNA 1 (head-to-head) |
1.01E+08 | 0.030334 | 0.000453 | 4.1003 | 0.007688 | 1.515 | LINC00958 | Long intergenic non-protein coding RNA 958 |
27146 | 0.026046 | 0.000327 | −4.2326 | 0.31073 | −1.7116 | FAM184B | Family with sequence similarity 184 member B |
3293 | 0.009849 | 0.000519 | −4.9792 | 2.0207 | −1.9794 | HSD17B3 | Hydroxysteroid 17-beta dehydrogenase 3 |
9172 | 0.03027 | 0.000447 | −4.1062 | 0.021258 | −2.0318 | MYOM2 | Myomesin 2 |
T lymphocyte (GSE6088) | | | | | | | |
6947 | 0.3014 | 0.014387 | −2.8309 | −2.8318 | −1.0073 | TCN1 | Transcobalamin 1 |
9022 | 0.20729 | 0.001842 | −3.9135 | −1.0713 | -1.0099 | CLIC3 | Chloride intracellular channel 3 |
9318 | 0.2472 | 0.007666 | -3.1611 | -2.2919 | -1.015 | COPS2 | COP9 signalosome subunit 2 |
3001 | 0.24454 | 0.006849 | -3.2201 | -2.1951 | -1.0251 | GZMA | Granzyme A |
6133 | 0.3272 | 0.020406 | -2.6466 | -3.1303 | -1.0317 | RPL9 | Ribosomal protein L9 |
1043 | 0.20262 | 0.001707 | -3.9542 | -1.0067 | -1.0424 | CD52 | CD52 molecule |
170622 | 0.24454 | 0.007035 | -3.2061 | -2.2181 | -1.0622 | COMMD6 | COMM domain containing 6 |
3904 | 0.23062 | 0.004078 | -3.4922 | -1.7503 | -1.0823 | LAIR2 | Leukocyte-associated immunoglobulin-like receptor 2 |
6201 | 0.32452 | 0.019401 | -2.6733 | -3.0872 | -1.0851 | RPS7 | Ribosomal protein S7 |
221687 | 0.47346 | 0.063946 | -2.0282 | -4.09 | -1.0976 | RNF182 | Ring finger protein 182 |
9326 | 0.20034 | 0.000504 | -4.6202 | 0.020066 | -1.1045 | ZNHIT3 | Zinc finger HIT-type containing 3 |
2123 | 0.23062 | 0.00412 | -3.4868 | -1.7591 | -1.1093 | EVI2A | Ecotropic viral integration site 2A |
55432 | 0.43923 | 0.051957 | -2.1434 | -3.9183 | -1.1175 | YOD1 | YOD1 deubiquitinase |
6164 | 0.23746 | 0.005617 | -3.324 | -2.025 | -1.1277 | RPL34 | Ribosomal protein L34 |
51187 | 0.23062 | 0.004557 | -3.4338 | -1.8455 | -1.1559 | RSL24D1 | Ribosomal L24 domain containing 1 |
23643 | 0.20034 | 0.000721 | -4.4216 | -0.27935 | -1.1582 | LY96 | Lymphocyte antigen 96 |
51348 | 0.24403 | 0.006614 | -3.2384 | -2.1651 | -1.2234 | KLRF1 | Killer cell lectin-like receptor F1 |
4697 | 0.22389 | 0.003022 | -3.6503 | -1.4938 | -1.2324 | NDUFA4 | NDUFA4 mitochondrial complex associated |
1349 | 0.27484 | 0.010668 | -2.9879 | -2.5755 | -1.2459 | COX7B | Cytochrome c oxidase subunit 7B |
8644 | 0.24454 | 0.006927 | -3.2142 | -2.2048 | -1.3589 | AKR1C3 | Aldo-keto reductase family 1 member C3 |
7504 | 0.48675 | 0.06987 | -1.9786 | -4.1627 | -1.3766 | XK | X-linked Kx blood group |
iPSC (GSE75545) | | | | | | | |
3127 | 0.055267 | 3.92E-06 | 8.9974 | -1.6805 | 1.2 | HLA-DRB5 | Major histocompatibility complex, class II, DR beta 5 |
55655 | 0.33643 | 4.78E-05 | -6.7624 | -2.019 | -2.5227 | NLRP2 | NLR family pyrin domain containing 2 |
Another commonly used method for comparing the two treatment conditions is the evaluation of the adjusted p-value to the logarithm of the fold change. The depicted diagram is commonly referred to as a volcano plot, owing to its resemblance to an erupting volcano, wherein clusters of data points are observed near the origin while exhibiting a dispersing pattern as they move away from this central position. Volcano plots are graphical representations that exhibit the statistical significance of the disparity to the extent of difference for each gene in the comparison. Typically, this is accomplished by employing the negative logarithm to base 10 for statistical significance and the logarithm to base 2 for fold change (Fig. 2). A broader distribution indicates a greater degree of disparity in gene expression between the two treatment groups. The occurrence of a volcano plot exhibiting a majority, or the entirety, of data points being densely clustered in proximity to the origin is a relatively infrequent phenomenon. MA plots exhibit the capacity to solely compare two distinct treatment conditions simultaneously.
Nevertheless, it is feasible to consolidate all pairwise comparisons about this specific illustration into a matrix configuration, allowing for the simultaneous representation of all conceivable combinations. This visualization facilitates the simultaneous monitoring of all pairwise comparisons between fold change and mean expression. Presents a methodology to ascertain the relative similarities or dissimilarities between treatment comparisons based on log-fold change and mean expression level. This approach, akin to the other matrix alternatives, allows users to visually depict their treatment-based comparisons. Genes exhibiting a log2(Fold Change) value exceeding zero can be classified as upregulated genes, while genes with a log2(Fold Change) value below zero can be categorized as downregulated. The nodes exhibiting a red coloration demonstrate a significant upregulation, whereas the nodes in yellow indicate a significant downregulation.
Network construction
The findings of the pathway enrichment analysis revealed that the Module has 32 nodes and 68 edges. These nodes and edges are largely concerned with the control of differentially expressed genes implicated in the development of atherosclerosis. Within the realm of protein networks, it is observed that protein partners within a cluster exhibit comparable functional attributes. Furthermore, these clusters rely heavily on hub genes, which are biologically interconnected nodes. The MCODE plugin, which is integrated into the Cytoscape software, detects and delineates clusters within a protein network. The MCODE cluster was subsequently augmented with eight prominent genes involved in lipid metabolism, namely APOB, LDLR, LDLRAP1, ABCA1, PCSK9, ABCG8, APOA1, APOC3, and ABCG5, in order to expand the protein-protein interaction network for further analysis. The FH nodes interacting with DEG edges possess an average STRINGDB interaction. The APOB binds to XK and LY96, APOA1 binds to LY96, and the PCSK9 binds to the OSM gene. GO annotations contain biological information about genes and the proteins they produce (Figs. 3 and 4).
GO and the annotation
The enricher demonstrates that the enrichment terms for the biological process are sterol transport (GO: 0015918), cholesterol homeostasis (GO: 0042632), and sterol homeostasis (GO: 0035092). Cholesterol Transfer Activity (GO: 0120020), Sterol Transfer Activity (GO:0120015), and Low-Density Lipoprotein Particle Receptor Binding (GO:0050750) are the enrichment terms from Molecular Function. Similar to CC, the enrichment terms are ATP-binding Cassette (ABC) Transporter Complex (GO:0043190), Clathrin-Coated Endocytic Vesicle Membrane (GO:0030669), and Clathrin-Coated Endocytic Vesicle (GO:0045334). The primary enrichment terms in Reactome include Chylomicron Clearance R-HAS (p-value >0.01, Fig. 5).27
Discussion
FH is distinguished by an elevated concentration of low-density lipoprotein cholesterol (LDL-C) in the bloodstream, which promotes the advancement of atherosclerosis. Atherosclerosis is widely recognized as a prominent risk factor for the development of coronary artery diseases, heart attacks, and strokes.28 An excessive amount of LDL particles leads to fibrous plaques to form in the subendothelial region, resulting in a reduction in a reduction in artery diameter. This constriction can ultimately result in heart ischemia and myocardial infarction. The primary genetic factors responsible for FH are the LDLR, APOB, and PCSK9 genes. The presence of a mutation in the LDLR gene impairs the interaction between the LDL receptor protein and the ApoB-LDL complex. Similarly, a mutation in the APOB gene prevents the ApoB protein from attaching to either LDL-C or the LDL receptor. The PCSK9 mutation enhances the degradation of LDL receptors. Mutations in these three genes together lead to elevated levels of LDL-C in the bloodstream. Another gene, APOE, participates in the lyophilization pathway and contributes to elevated levels of LDL-C in the bloodstream. The CELSRZ protein is implicated in proteolytic glycosylation, which contributes to elevated levels of LDL-C in the bloodstream. The proper establishment of HFE, NYNRIN, MYLIP with FH has not been completed. In this investigation, we employed an integrated bioinformatics methodology. We utilized various tools to ascertain potential pathways and genes that may significantly impact the progression of FH. Using the Limma expression in networkanalyst.ca, we discovered 743 DEGs in the monocyte and t-lymphocytes datasets and 691 DEGs in the iPSCs dataset. Upregulated genes included MYO18B, LINC00644, CYS1, CLDN8, RGPD4-AS1, LINC00958, and HLA-DRB5. Downregulated genes included FAM184B, HSD17B3, MYOM2, TCN1, CLIC3, COPS2, GZMA, RPL9, CD52, COMMD6, LAIR2, RPS7, RNF182, ZNHIT3, EVI2A, YOD1, RPL34, RSL24D1, LY96, KLRF1, NDUFA4, COX7B, AKR1C3, XK, and NLRP2. The RSP7 genes were found in all 4 pathways in the ENRICH tool, followed by AKRIC3, PL9 and OSM (found in Reactome, BP and CC). Oncostatin M, an IL-6 cytokine, has been demonstrated to potently influence the activation of LDLR expression in HepG2 cells through a non-SREBP-mediated route. The transcription factors Egr1 and c/EBP bind to the SIRE region of the promoter, which is located downstream of the SRE-1 region, making it easier to stimulate LDLR gene transcription. However, the other 3 genes were not directly involved in the LDL pathway; any connection may be between these three genes and FH, which needs to be focused. According to the Venn diagram, 30 DEGs were common to monocyte and iPSc, 59 DEGs were common in monocyte and t-lymphocyte, and 31 DEGs were common to t-lymphocyte and iPSCs. 4 DEGs, NCOR2, NMD3, POLR2A and RPE, appeared in all three datasets. The human NMD3 gene generates proteinaceous nucleolar protein NMD3. It is best recognized for component synthesis and assembly in ribosome biogenesis. SMRT (silence mediator for retinoid or thyroid hormone receptors) modulates nuclear receptor-mediated transcriptional activity. Several transcription factors are simultaneously inhibited. POLR2A encodes Rpb1, the largest eukaryotic Pol II component. DNA templates are transcribed into mRNA by RNA polymerase II. The RPE gene encodes ribulose-5-phosphate-3-epimerase. In the pentose phosphate pathway, the enzyme produces ribose-5-phosphate, a precursor for nucleotide and nucleic acid biosynthesis. The system creates NADPH, which is required for fatty acid synthesis and reactive oxygen species neutralization.
The PPI network reveals that PCSK9 interacts with KLRF1 (a gene that encodes for a receptor that is predominantly expressed on natural killer cells), while APOB interacts with Xk and LY96. The XK gene encodes the XK protein, a membrane transporter protein predominantly expressed in erythrocytes.29 The Kell blood group complex encompasses a constituent that is critical in maintaining the integrity and functionality of the erythrocyte membrane. Scientists have recently commenced the analysis of the impact of XK in diverse cardiovascular disorders. For instance, the Toll-like receptor 4 (TLR4) has been associated with lipid metabolism and atherosclerosis, indicating potential implications in the development of CVD.30 Given the close association between LY96 (MD-2) and TLR4 signaling, it is plausible to hypothesize that it may have an indirect effect on cholesterol metabolism or other related physiological processes. Based on the findings of our investigation, it is proposed that the observed increase in the activity of specific genes (CCL3, MYO18B, LINC00644, CYS1, CLDN8, RGPD4-AS1, LINC00958, and HLA-DRB5) and the subsequent overexpression of their corresponding receptors may potentially encompass a wide array of interconnected pathways, intricately intertwined with diverse cascades such as inflammation and innate immunity.31 The genes investigated in this study, along with their corresponding functions, are listed in Table 2. Prior studies have established the importance of identifying genes that are expressed differently (DEGs) in a specific ailment, as it can substantially assist in the diagnostic process.32,33 Nevertheless, these 10 genes are not directly associated with the FH condition. However, this provides a potential opportunity to evaluate FH.34 Through the utilization of Reverse Transcription Polymerase Chain Reaction (RT-PCR), we can examine the expression of these 10 genes in FH-affected patients to reveal their genuine influence (Fig. 6).
Table 2Functions and location of the identified differentially expressed genes and their role in cardiovascular diseases
Gene | Function | Location | Relation to cardiovascular disease |
---|
KLRF1 | Facilitates class 1 MHC receptor activity, carbohydrate binding, transmembrane signaling receptor activity, and plays a role in cell surface receptor signaling pathway | 12p13.31 | Significant risk factors for cardiovascular disease include the KLRF1 gene, which may influence blood pressure and the inflammatory response in the cardiovascular system |
Xk | Facilitates protein binding and acts as an adaptor for protein macromolecules. Engaged in the study of AA transport, intracellular calcium and magnesium ion balance, myelination, the regulation of axon diameter and cell size, and muscle fiber development | Xp21.1 | The XK gene’s involvement in CVD may be connected to its function in preserving the health and functionality of red blood cells. McLeod Syndrome can result from mutations in the XK gene |
LY96 | Permits the binding of TLR4, coreceptors, LPS, and LPS immune receptors | 8q21.11 | The LY96 gene plays a function in CVD by inducing inflammatory processes that aid in the formation and advancement of atherosclerosis |
AKRIC3 | It carries out prostaglandin synthase functions | 10p15.1 | The AKRIC3 gene interacts with signaling molecules linked to cardiac hypertrophy, cystic fibrosis, and apoptosis, which may contribute to the onset and progression of CVD |
OSM | Controls the function of neurons | 22q12.2 | OSM may aid in the development of atherosclerotic calcification by promoting osteoblastic differentiation of vascular smooth muscle cells via the JAK3/STAT3 pathway |
NCOR2 | Control B cell proliferation and preserve genomic integrity | 12q24.31 | The NCOR2 gene plays a role in CVD by controlling lipid metabolism, modifying inflammation, and affecting heart function |
NMD3 | Serves as an adapter to allow the 60S ribosomal subunit to be exported from the nucleus | 3q26.1 | NMD3 is involved in pathogenesis of cellular tissue in Heart walls |
POLR2A | Interacts with CREB1 to inhibit osteoclastic bone resorption and prevent osteoporosis | 17p13.1 | The biggest subunit of RNA polymerase II, POLR2A, is encoded and is involved in the transcription of many different genes. Dysregulation of the POLR2A gene can affect the expression of genes linked to cardiovascular disease and atherosclerosis |
RPE | The visual cycle involves the following processes: phagocytosis of shed photoreceptor membranes; re-isomerization of all-trans-retinal into 11-cis-retinal; transport of nutrients, ions, and water; absorption of light and protection against photooxidation | 2q34 | The role of the RPE gene in lipid metabolism and inflammation is crucial in understanding the development of atherosclerosis, a major contributor to cardiovascular
disease |
RPS7 | Facilitates the binding of additional ribosomal proteins to form the head of the 30S subunit by organizing the folding of the 16S rRNA 3′ major domain | 2p25.3 | Not Directly involved |
Conclusions
The present investigation has identified genes that exhibit differential expression in individuals diagnosed with FH and are associated with the development of atherosclerosis compared to healthy individuals. The empirical data demonstrates a significant correlation between the three genes, KLRF1, Xk and LY96, as well as the FH genes. The genes exhibiting an elevated expression level are RPS7, AKRIC3, PL9, and OSM. These genes are of utmost importance in ribosomal transcription and the monitoring of transcription and immune responses. To enhance our comprehension of this phenomenon, the analysis of the roles of these genes in FH with atherosclerosis is necessary. The present study also establishes a foundation for investigating potential therapeutic targets that could alleviate the impact of CVD in individuals diagnosed with FH.
Abbreviations
- ABC:
ATP-binding Cassette
- ABCG5:
ATP-Binding Cassette Subfamily G Member 5
- ABCG8:
ATP-Binding Cassette Subfamily G Member 8
- AKR1C3:
Aldo-Keto Reductase Family 1 Member C3
- APOB:
Apolipoprotein B
- APOE:
Apolipoprotein E
- BPs:
Biological Processes
- CC:
Cellular Components
- CELSR2:
Cadherin EGF LAG Seven-Pass G-Type Receptor 2
- COPS2:
COP9 Signalosome Subunit 2
- COX7B:
Cytochrome C Oxidase Subunit 7B
- CVD:
cardiovascular disease
- DEGs:
differentially expressed genes
- FH:
familial hypercholesterolemia
- GEO:
Gene Expression Omnibus
- GO:
gene ontology
- GZMA:
Granzyme A
- HLA:
Human Leukocyte Antigen.
- HSD17B3:
Hydroxysteroid 17-Beta Dehydrogenase 3
- KLRF1:
Killer Cell Lectin Like Receptor F1
- LDL-C:
low-density lipoprotein cholesterol
- LDLR:
Low-Density Lipoprotein Receptor
- LDLRAP1:
Low-Density Lipoprotein Receptor Adaptor Protein 1
- LY96:
Lymphocyte Antigen 96
- MA plot:
M vs A plot
- MCODE:
Molecular Complex Detection
- MFs:
Molecular Functions
- MYLIP:
Myosin Regulatory Light Chain Interacting Protein
- MYO18B:
Myosin XVIIIB
- NCOR2:
Nuclear Receptor Corepressor 2
- NDUFA4:
NADH: Ubiquinone Oxidoreductase Subunit A4
- NLR:
NOD-like receptor
- NMD3:
Ribosome Exporting Factor
- OSM:
Oncostatin M
- PCSK9:
Proprotein Convertase Subtilisin/Kexin Type 9
- PEX6:
Peroxisomal Biogenesis Factor 6
- PL9:
Phospholamban (PLN)
- POLR2A:
RNA Polymerase II Subunit A
- RPL34:
Ribosomal Protein L34
- RPL9:
Ribosomal Protein L9
- RPS9:
Ribosomal Protein S9
- RSL24D1:
Ribosomal L24 Domain Containing 1
- TCN1:
Transcobalamin 1
- TLR4:
Toll-like receptor 4
- XK:
X-Linked Kx Blood Group
- YOD1:
Deubiquitinase OTU1
- ZNHIT3:
Zinc Finger HIT-Type Containing 3
Declarations
Acknowledgement
All the work has been done from authors involved. KK acknowledges Department of Genetic Engineering, SRMIST for Scholar support.
Data sharing statement
The data that support the findings of this study are available from the corresponding author, [Dr. KN ArulJothi], upon reasonable request. All the datasets are retrieved from NCBI Geo Datasets.
Funding
This study received no specific grant from any funding agency in the public, commercial, or not-for-profit sector.
Conflict of interest
The authors have no conflict of interests related to this publication.
Authors’ contributions
KK and KNA involved in designing of the work and drafting the manuscript. JSK involved in critical revision of the paper. All authors have given approval to the final version of the manuscript.