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Effect of Exercise on NAFLD and Its Risk Factors: Comparison of Moderate versus Low Intensity Exercise

  • Preetam Nath1,
  • Manas Kumar Panigrahi2,
  • Manoj Kumar Sahu3,
  • Jimmy Narayan3,
  • Ranjan Kumar Sahoo4,
  • Ananya Apurba Patra4,
  • Satyaswarup Jena5,
  • Arun Kumar Patnaik6,
  • Anjan Jena6 and
  • Shivaram Prasad Singh*,7
Journal of Clinical and Translational Hepatology   2020;8(2):120-126

doi: 10.14218/JCTH.2019.00012

Received:

Revised:

Accepted:

Published online:

 Author information

Citation: Nath P, Panigrahi MK, Sahu MK, Narayan J, Sahoo RK, Patra AA, et al. Effect of Exercise on NAFLD and Its Risk Factors: Comparison of Moderate versus Low Intensity Exercise. J Clin Transl Hepatol. 2020;8(2):120-126. doi: 10.14218/JCTH.2019.00012.

Abstract

Background and Aims: Lifestyle (exercise and dietary) modification is the mainstay of treatment for non-alcoholic fatty liver disease (NAFLD). However, there is paucity of data on effect of intensity of exercise in management of NAFLD, and we aimed to study the effect of variable intensities of exercise on NAFLD.

Methods: The study was performed in the Department of Gastroenterology of the SCB Medical College, Cuttack and the Biju Patnaik State Police Academy, Bhubaneswar. The subjects were police trainees [18 in a moderate intensity exercise group (MIG) and 19 in a low intensity exercise group (LIG)] recruited for a 6-month physical training course (261.8 Kcalorie, 3.6 metabolic equivalent in MIG and 153.6 Kcalorie, 2.1 metabolic equivalent in LIG). NAFLD was diagnosed by ultrasonography, with exclusion of all secondary causes of steatosis. All participants were evaluated by anthropometry (weight, height, body mass index (BMI), waist circumference), assessed for blood pressure and biochemical parameters (blood glucose, liver function test, lipid profile, serum insulin), and subjected to transabdominal ultrasonography before and after 6 months of physical training, and the results were compared.

Results: Both the groups had similar BMI, fasting plasma glucose, AST, gamma-glutamyl transpeptidase, insulin, and homeostatic model assessment-insulin resistance (known as HOMA-IR) (p>0.05). However, subjects in the LIG were older and had lower alanine transaminase, higher triglycerides and lower high-density lipoproteins than MIG subjects. There was a significant reduction in BMI (27.0±2.1 to 26.8±2.0; p=0.001), fasting blood glucose (106.7±21.6 to 85.8±19.0; p<0.001), serum triglycerides (167.5±56.7 to 124.6±63.5; p=0.017), total cholesterol (216.8±29.2 to 196.7±26.6; p=0.037), low-density lipoprotein cholesterol (134.6±21.4 to 130.5±21.9; p=0.010), serum aspartate transaminase (39.3±32.2 to 30.9±11.4; p<0.001), serum alanine transaminase (56.6±28.7 to 33.0±11.3; p<0.001) and HOMA-IR (2.63±2.66 to 1.70±2.59; p<0.001) in the MIG. However, changes in these parameters in the LIG were non-significant. Hepatic steatosis regressed in 66.7% of the NAFLD subjects in the MIG but in only 26.3% of the LIG NAFLD subjects (p=0.030).

Conclusions: Moderate rather than low intensity physical activity causes significant improvement in BMI, serum triglycerides, cholesterol, serum transaminases and HOMA-IR, and regression of ultrasonographic fatty change in liver among NAFLD subjects.

Keywords

Exercise, NAFLD, Intensity, Physical activity

Introduction

Non-alcoholic fatty liver disease (NAFLD) is one of the most common hepatic disorders, with macrovesicular fat accumulation in more than 5% of hepatocytes in the absence of any secondary cause of hepatic steatosis, such as significant alcohol abuse (more than 10 g/day for women and 20 g/day for men), hepatotropic viral infection, drugs which can cause fatty liver, or any other etiologies.1 In the last couple of decades, NAFLD has emerged as the most common liver disease in adults.2 Its prevalence varies in different parts of the world, ranging from 5-40%. However, its occurrence is even higher in individuals with obesity (30-100%) and type 2 diabetes mellitus (42.6-69.5%).3 Despite the benign natural course in the majority, 10% of the NAFLD patients may progress to cirrhosis later on in their lives.4 It contributes to formation of a pro-inflammatory environment that accelerates atherosclerosis, increasing the risk of ischemic heart disease and its severity, which itself correlates with the degree of inflammation.5 In addition, these individuals are at a high risk for developing diabetes mellitus. Hence, patient education about the entity, need for treatment and compliance are necessary to avert future complications.

Lifestyle modification is still the treatment of first choice for NAFLD patients and is recommended for all patients with NAFLD.6 This aims at weight loss and reduction of insulin resistance (IR). Further, it is also directed at managing obesity and the features of metabolic syndrome which are frequently associated with NAFLD. Low-calorie diet and increased physical activity are the cornerstones of lifestyle modification. Patients are encouraged to lose at least 10% of their initial body weight to achieve maximal benefits. Weight loss improves patients’ cardiovascular risk profile and steatosis,7 and probably reduces hepatic inflammation as well as hepatocellular injury.8 Exercise is a major component of treatment for NAFLD, as recommended by the American Gastroenterological Association,9 the American Association for the Study of Liver Diseases and the European Association of Study of Liver. As compared to type 2 diabetes mellitus, there is a paucity of data supporting the role of physical activity in management of NAFLD. This may be due to the invasive nature of grading hepatic steatosis by needle biopsy and histology, which limits the capacity for repeated measurement of hepatic steatosis and degree of necroinflammation. The federal guidelines of the USA’s Department of Health and Human Services and the USA’s Department of Agriculture recommend that adults should perform 150 min or more of moderate-intensity physical activity per week, 75 min or more of vigorous intensity physical activity per week, or a combination, to improve and maintain health.10 The Centers for Disease Control and Prevention and the World Health Organization11 recommendations are also the same. For additional health benefits, the amount of physical activity recommended should be doubled.11 However, controversy remains over the role of exercise intensity and total volume of exercise responsible for final health outcomes.

The aim of this study was to compare the beneficial effects of low and moderate intensity exercise for 6 months, on NAFLD.

Methods

This study was performed in the Biju Patnaik State Police Academy, Bhubaneswar, Odisha, India which conducts 6 months of physical training before recruitment or promotion. The subjects were middle-aged male police recruits selected for 6 months of physical training.

Inclusion criteria

The police trainees who had fatty liver detected on ultrasonography were included in this study. These participants were in good health and had no abnormal findings on general and systemic physical examinations and for blood count and transabdominal ultrasonography (except for fatty liver).

Exclusion criteria

Patients with organic gastrointestinal disease revealed by ultrasonography or gastroduodenoscopy were excluded. Participants who had history of alcohol consumption exceeding 20 g⁄day, subjects with other liver diseases [hepatitis viruses A through E (by viral serologies: hepatitis B surface antigen, anti-hepatitis C virus antibody), autoimmune disease (autoimmune markers: anti-nuclear antibody, anti-smooth muscle antibody, anti-liver kidney microsome type 1 antibody) and Wilson’s disease (serum ceruloplasmin)] and those on drugs which can induce fatty liver or insulin sensitization (estrogens, amiodarone, methotrexate, tamoxifen, glitazones, metformin) were also excluded from the study.

An informed consent was obtained from each participant. Detailed anthropometric assessment, including measurements of weight, height, and waist and hip circumferences, was conducted before and after 6 months of physical exercise. The waist circumference was measured at a level midway between the lowest rib and the iliac crest and hip circumference at the level of the greater trochanter. Body mass index (BMI) was calculated by the formula of weight (kg) / height2 (m2).

The measurements of fasting plasma glucose, 2-h post-glucose load plasma glucose, serum triglycerides, serum total cholesterol, serum high-density lipoprotein cholesterol and liver function markers were performed by standard laboratory methods both before the beginning and after the completion of 6 months of physical exercise. The estimation of serum insulin was performed by using the electrochemiluminescence method (Roche-Diagnostics, USA) with an autoanalyzer, Elecsys 2010 (Roche-Hitachi, Japan). IR was computed by using the homeostatic model assessment (HOMA) method via a mathematical model derived from fasting plasma glucose and plasma insulin. The value of HOMA was calculated by the following equation: [fasting insulin (μU/mL) × fasting blood glucose (mg/dL)]/405, and depicted as the HOMA-IR value.12 For the purpose of the study, a HOMA-IR value above 2 was considered to indicate IR.13

Transabdominal ultrasonography was performed independently and blindly by two experienced radiologists (AAP and SJ; 5-10 years’ experience) to identify and grade fatty changes in liver; in case of any conflict, the discrepancy was resolved by consensus after examination by a third senior radiologist (RKS; 15 years’ experience). The diagnosis of fatty liver was made as per the standard criteria adopted by the American Gastroenterology Association,14 i.e. an increase in liver echogenicity as compared to renal echogenicity as a reference, as well as lack of differentiation in periportal intensity and vascular wall due to hyperechogenicity of the liver parenchyma.

All the participants were subjected to aerobic physical training for 6 months, which included brisk walking, jogging, marching drill, ‘lathi’ drill, and yoga. The duration of each session of the physical activity was 50-60 m per day with a frequency of 5-6 sessions per week. The duration of physical activity and total calories burnt during exercise (volume of exercise) were estimated by accelerometers. The intensity of exercise was deducted by the equation for metabolic equivalent (MET) as follows: (total calories)/(kg body weight)/(hours of exercised performed). Activities with MET values below 3 were labelled as low intensity exercise, those with MET between 3 and 5.9 were classified as moderate intensity, and activities with MET values ≥ 6 were classified as vigorous intensity.

The subjects of the study were classified into the following groups:

1. Low intensity exercise group (LIG) and

2. Moderate intensity exercise group (MIG).

None of the study participants were subjected to vigorous intensity exercise. All the trainees were provided with a diet of 2400 to 2700 kcal/day. This study was approved by the Institutional Ethics Committee of SCB Medical College, Cuttack.

Statistical analysis

Normally distributed continuous variables were expressed as mean±standard deviation. Student’s t-test for unpaired data was used to compare the groups when the variables were normally distributed. Chi-square test was used to compare differences between categorical variables. All the anthropometric and biochemical parameters before and after 6 months of physical activity were compared by Student’s t-test for paired data. All calculations were performed using the statistical software, SPSS version 16. A p value of less than 0.05 was taken as significant.

Results

Out of 62 police recruits, a total of 37 subjects were to found to have NAFLD, out of which 18 performed moderate intensity exercise and 19 performed low intensity exercise for 6 months (Fig. 1). Baseline comparison between the two groups is depicted in Table 1. The mean intensity of physical activity in the MIG was 3.6 MET (with mean total volume of exercise: 261.8 Kcalorie), whereas that of the LIG was 2.1 MET (with mean volume: 156.6 Kcalorie).

Study consort diagram.
Fig. 1.  Study consort diagram.
Table 1.

Baseline characteristics of NAFLD subjects before 6 months of physical training

ParametersLow intensity exercise, n=19Moderate intensity exercise, n=18p value
Age (years)53.3±3.737.3±8.2<0.001
BMI (kg/m2)26.1±2.126.9±1.90.257
SBP (mm Hg)156.6±18.9132.2±13.2<0.001
DBP (mm Hg)90.7±10.187.4±8.30.339
FPG (mg/dL)183.7±79.2104±20.70.224
PGPG (mg/dL)185.8±67.4145.2±60.20.090
Triglycerides (mg/dL)186.2±72.6157.9±57.20.248
Cholesterol (mg/dL)181.4±34.8211.2±30.00.018
HDL (mg/dL)42.1±8.946.0±4.80.159
LDL (mg/dL)102.5±29.9130.5±22.00.007
VLDL (mg/dL)36.8±14.534.6±16.80.669
Bilirubin (mg/dL)1.1±0.70.8±0.30.225
AST (U/L)23.9±7.939.2±29.50.039
ALT (U/L)26.3±13.161.8±30.2<0.001
ALP (U/L)189.6±37.4202.7±51.30.409
GGT (U/L)52.7±49.661.3±51.30.639
HOMA IR3.75±5.272.56±2.550.458
Exercise duration (minutes)57.21±4.2256.57±6.870.728
Total calories burnt in exercise153.58±29.10261.81±37.18<0.001
MET2.11±0.273.61±0.42<0.001

Patients in the LIG were older (53.3±3.7 years vs. 37.3±8.2 years; p<0.001), with significantly higher systolic blood pressure (156.6±18.9 mmHg vs. 132.2±13.2 mmHg; p<0.001), lower serum total cholesterol (181.4±34.8 vs. 211.2±30.0; p=0.018), lower low-density lipoprotein (LDL) cholesterol (102.5±29.9 vs. 30.5±22.0; p=0.007), lower serum aspartate transaminase (AST; 23.9±7.9 vs. 39.2±29.5; p=0.039) and lower serum alanine transaminase (ALT; 26.3±13.1 vs. 61.8±30.2; p<0.001). However, both of the groups had comparable BMI, diastolic blood pressure, fasting and 2-h post-glucose load plasma glucose, serum triglycerides, high-density lipoprotein, very low-density lipoprotein, bilirubin, alkaline phosphatase, gamma-glutamyl transpeptidase (GGT), HOMA-IR, 24-h dietary intake, and total duration of physical activity. On the other hand, subjects with moderate intensity exercise had significantly higher total calories burnt in exercise (261.81±37.18 Kcalorie vs. 153.58±29.10 Kcalorie; p<0.001).

In the LIG, there was significant decrease in waist hip ratio and waist height ratio, whereas decrease in mean BMI was modest (0.2) and non-significant (p=0.623) (Table 2). Besides, there was a significant reduction in blood pressure, serum total, high-density lipoprotein, very low-density lipoprotein cholesterol, and serum GGT. However, changes in all other parameters, including serum transaminases (AST and ALT), were non-significant. Furthermore, only 5 out of 19 (26.3%) had resolution of fatty change in liver on ultrasonography.

Table 2.

Changes of parameters after 6 months of physical training in the low intensity exercise group and moderate intensity exercise group

ParametersLow intensity exercise group (n=19)Moderate intensity exercise group (n=18)
Before 6 months of physical trainingAfter 6 months of physical trainingp valueBefore 6 months of physical trainingAfter 6 months of physical trainingp value
BMI (kg/m2)26.1±2.125.9±1.80.62326.9±1.926.7±2.00.428
Waist hip ratio1.01±0.350.93±0.22< 0.0010.97±0.040.96±0.040.262
Waist height ratio0.59±0.040.57±0.4< 0.0010.56±0.040.55±0.040.168
SBP (mm Hg)156.6±18.9140.1±21.3< 0.001132.2±13.2116.4±8.90.001
DBP (mm Hg)90.7±10.185.8±11.10.01887.4±8.377.8±8.60.005
FPG (mg/dL)183.7±79.2129.7±48.30.333104±20.785.8±19.0< 0.001
PGPG (mg/dL)185.8±67.4185.1±85.50.961145.2±60.2120.1±40.80.068
Triglycerides (mg/dL)186.2±72.6166.8±77.80.303157.9±57.2124.6±63.50.013
Cholesterol (mg/dL)181.4±34.8167.1±33.20.009211.2±30.0196.7±26.60.020
HDL (mg/dL)42.1±8.937.8±6.70.00146.0±4.841.5±6.80.006
LDL (mg/dL)102.5±29.999.4±26.60.571130.5±22.0130.5±21.90.398
VLDL (mg/dL)36.8±14.529.8±11.60.03234.6±16.824.8±12.70.010
Bilirubin (mg/dL)1.1±0.70.9±0.80.1330.8±0.30.7 ±0.40.190
AST (U/L)23.9±7.922.0±5.70.20439.2±29.529.8±8.50.253
ALT (U/L)26.3±13.122.6±6.60.20561.8±30.229.1±9.80.002
ALP (U/L)189.6±37.4176.3±18.30.137202.7±51.3193.2±50.90.440
GGT (U/L)52.7±49.636.7±25.70.03361.3±51.337.4±24.10.024
HOMA-IR3.75±5.272.71±2.600.4662.56±2.551.69±2.590.005

Similarly, for the subjects who were subjected to moderate intensity exercise—although they showed modest improvement in BMI, waist hip ratio and waist height ratio, these changes were non-significant. However, there was significant reduction in blood pressure (Table 2). Further, there was significant improvement in lipid profile (except LDL cholesterol) and serum GGT. Unlike the LIG, subjects with moderate intensity exercise had elevated serum ALT at the baseline, and there were significant drops in serum levels of the transaminases (both AST and ALT) and decrease in HOMA-IR. Similarly, fasting glucose and triglycerides were normal/borderline normal at baseline in the MIG, which decreased significantly after intervention; whereas, in the LIG, these parameters were abnormal, with no significant change. Moreover, the fatty changes in liver disappeared in 12 out 18 (66.7%) subjects. The percentage of non-significant (p>0.05) decrease in weight was higher in those who achieved resolution of fatty change in liver (Table 3).

Table 3.

Comparison of percentage of change in weight in the study groups

Subjects with regression of fatty liverSubjects without regression of fatty liverp value
All subjects-2.28%-1.03%0.305
Low intensity exercise group-0.20%-0.11%0.946
Moderate intensity exercise group-3.19%-2.97%0.916

Univariate logistic regression analysis was performed to assess the risk factors for resolution of fatty change in liver after physical training (Table 4). Out of all demographic, anthropometric, biochemical parameters and exercise indices, serum triglycerides, very low-density lipoprotein, presence of metabolic syndrome and exercise intensity were found to be significant predictors for response (sonographic resolution of fatty change in liver) to physical exercise in NAFLD participants. A multivariate logistic regression analysis was performed including the above risk factors (Table 4) and only exercise intensity was found to be an independent factor for resolution of fatty changes in liver on ultrasound. The non-invasive scores of fibrosis [NAFLD fibrosis score, fibrosis-4 score, and AST to platelet ratio (commonly referred to as APRI)] as well as fatty liver index (FLI) were computed for all study subjects. Participants in the MIG showed significant improvement in APRI score (p=0.005) and FLI (p<0.001) (Table 5). However, in the subjects belonging to the LIG, no significant improvement was observed in any of the scores, except for reduction in FLI (p=0.031) after physical training (Table 5).

Table 4.

Logistic (univariate and multivariate) regression analysis of factors predicting resolution of fatty changes in liver in NAFLD subjects (n=37)

FactorsOR95% CIp valueAOR95% CIp value
Age (years)1.020.93-1.120.6731.070.92-1.260.383
BMI (kg/m2)1.020.73-1.430.908
Waist circumference0.940.84-1.060.302
Waist hip ratio0.0020.0001-15460.443
Waist height ratio0.540.0001-735660.942
SBP (mm Hg)0.980.95-1.010.262
DBP (mm Hg)1.010.93-1.070.985
FPG (mg/dL)0.980.96-1.010.057
PGPG (mg/dL)0.990.98-1.000.106
Triglycerides (mg/dL)0.980.96-0.990.0090.580.26-1.310.189
Cholesterol (mg/dL)1.010.98-1.030.631
HDL (mg/dL)1.040.95-1.140.362
LDL (mg/dL)1.020.99-1.040.158
VLDL (mg/dL)0.900.83-0.980.01213.840.24-80.790.205
Bilirubin (mg/dL)0.260.02-3.510.311
AST (U/L)1.030.98-1.080.324
ALT (U/L)1.010.98-1.040.340
ALP (U/L)1.010.99-1.020.457
GGT (U/L)1.010.99-1.020.490
Fasting insulin0.870.69-1.070.191
HOMA-IR0.520.24-1.110.089
Metabolic Syndrome4.291.06-17.360.0410.9100.13-6.550.926
Total duration of exercise1.010.89-1.290.978
Total calories burnt during exercise1.010.98-1.020.122
Exercise intensity (low vs. moderate)0.180.04-0.740.01717.181.16-253.610.038
MET2.351.01-5.470.043
Table 5.

Non-invasive fibrosis scores of NAFLD and FLI before and after exercise

ScoreModerate intensity groupLow intensity group
Before exerciseAfter exercisep valueBefore exerciseAfter exercisep value
NFS-1.35-1.590.2780.660.750.621
FIB-40.840.810.7220.880.860.772
APRI0.410.270.0050.430.400.285
FLI62.8450.55<0.00166.0860.430.031

Discussion

The MIG had an elevated ALT and borderline normal fasting glucose and serum triglycerides at baseline, with significant improvement in these parameters at the end of the 6-month period. However, the LIG had normal ALT and abnormal fasting glucose and serum triglycerides at baseline but no significant change after intervention. The basic principle underlying use of physical exercise in treatment of NAFLD is improvement in insulin sensitivity. Moderate to vigorous physical activity leads to reduction in total body fat, especially in visceral adipose tissue.15 Decreased visceral adiposity leads to reduced fatty acid delivery to the liver, thereby decreasing hepatic steatosis and resultant necroinflammation.

In our study, moderate intensity aerobic exercise resulted in significant resolution of fatty change in liver and improvement in serum aminotransferases (AST and ALT). The role of physical activity in NAFLD has been investigated in various studies. Suzuki et al.16 demonstrated that regular exercise was associated with ALT reduction. Further, for every 5% in weight loss, a 3.6 greater likelihood of ALT normalization was observed in their study.16 Studies employing magnetic resonance spectroscopy (known as 1H-MRS) also confirm these reports.17,18 In our study, although the degree of weight loss in both the groups was not significant, there was a significant fall in serum transaminases in the MIG. However, in the LIG, the reduction in transaminases was not significant.

The effect of exercise with or without hypocaloric diet on NAFLD patients has been studied in various clinical trials.8,19–27 Most of these studies had smaller number of subjects, absence of measurement of volume (calorie) and intensity of exercise, and some lacked histological endpoints. A comparison of the results of eight studies, including the present study, is shown in Supplementary Table 1. All trials showed significant improvement in BMI, serum enzymes (AST/ALT), and degree of fatty liver. There are several studies that have demonstrated a dose-dependent improvement in liver histology27 and intrahepatic triglycerides26 dependent on the degree of weight loss achieved. However, the improvement in transaminases, HOMA-IR, FLI and resolution of ultrasonographic fatty change in liver was independent of the degree of weight loss in our study. Further studies are required, with longer duration and with more intense physical activities, to demonstrate changes in fibrosis score.

Recently, the intensity rather than duration of exercise and total calorie expenditure during physical activity has caught the attention of researchers. Kistler et al.23 in a retrospective study examined the effect of exercise intensity on histological severity of NAFLD. In this study, the exercise volume and intensity was calculated by self-reported physical activity data from adult patients with biopsy-proven NAFLD enrolled in the Nonalcoholic Steatohepatitis Clinical Research Network, and the NAFLD patients were classified into moderate and vigorous exercise groups as per the federal recommendations. The study demonstrated an inverse relationship between the intensity of physical activity and severity of NAFLD. However, this study was limited by its cross-sectional nature, measurement limitations, and misclassification due to reporting and recall bias. On the contrary, in our study, we measured the exercise intensity prospectively with objective methods (by accelerometers).

Our study had several limitations. The two study groups were not entirely comparable as there were significant differences in age, baseline levels of serum AST and ALT, serum total cholesterol, and serum LDL cholesterol. Besides, the sample size was small and only male NAFLD subjects were included. Furthermore, the diagnosis of NAFLD was based on transabdominal ultrasound, which can miss fatty liver when the degree of steatosis is less than 30%.28

Conclusions

Moderate intensity of physical activities for a duration of 6 months helps in improvement in hepatic steatosis, serum transaminitis, glycemic and lipid profiles as well as IR, as compared with low intensity exercise. All individuals with NAFLD should perform moderately intense physical activities for maximum benefits. More rigorous, controlled studies, of longer duration and with defined histopathological end-points are the need of the hour for better evidence-based lifestyle modification guidelines.

Supplementary Information

Supplementary Table 1

Comparison of various studies assessing effect of exercise on NAFLD

(DOCX)

Abbreviations

1H-MRS: 

magnetic resonance spectroscopy

ALT: 

alanine transaminase

APRI: 

AST to platelet ratio

AST: 

aspartate transaminase

BMI: 

body mass index

FLI: 

fatty liver index

GGT: 

gamma-glutamyl transpeptidase

HOMA: 

homeostatic model assessment

IR: 

insulin resistance

LDL: 

low-density lipoprotein

LIG: 

low intensity exercise group

MET: 

metabolic equivalent

MIG: 

moderate intensity exercise group

NAFLD: 

non-alcoholic fatty liver disease

Declarations

Funding

This study was sponsored by the Kalinga Gastroenterology Foundation, Cuttack, Odisha (India).

Conflict of interest

The authors have no conflict of interests related to this publication.

Authors’ contributions

Study concept and design (PN, SPS), acquisition of data (PN, MKS, JN, RKS, AAP, SJ, AKP, AJ), analysis and interpretation of data (PN, MKP, SPS), drafting of the manuscript (PN, MKP), critical revision of the manuscript for important intellectual content (PN, MKP, JN, SPS), study supervision (SPS).

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