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miR-139-5p Regulates PIEZO1 Expression to Modulate Radiation-induced Injury in Type II Alveolar Epithelial Cells: A Hypothesis

  • Jiaqi Huang1,
  • Yan Lu2,
  • Hao Zhang3,
  • Sinian Wang4,
  • Xuewei Guo5,
  • Xiaoli Lv6,
  • Shinan Wei7 and
  • Yongwang Li1,* 
Exploratory Research and Hypothesis in Medicine   2023;8(1):65-70

doi: 10.14218/ERHM.2022.00063

Received:

Revised:

Accepted:

Published online:

 Author information

Citation: Huang J, Lu Y, Zhang H, Wang S, Guo X, Lv X, et al. miR-139-5p Regulates PIEZO1 Expression to Modulate Radiation-induced Injury in Type II Alveolar Epithelial Cells: A Hypothesis. Explor Res Hypothesis Med. 2023;8(1):65-70. doi: 10.14218/ERHM.2022.00063.

Abstract

Radiation-induced pulmonary injury (RIPI) is a common adverse reaction when ionizing radiation acts on the lung. Type II alveolar epithelial cells participate in the process of RIPI by regulating inflammation, epithelial-mesenchymal transition, cellular senescence, etc. The expression of miR-139-5p is inhibited by ionizing radiation, which plays a role in modulating radiotherapy resistance in breast cancer tissues. PIEZO1, a mechano-sensitive ion channel, has been found to play an essential role in bleomycin-induced lung fibrosis. Moreover, there exist some common mechanisms between bleomycin-induced lung fibrosis and RIPI. The stretch changes during RIPI might also regulate PIEZO1 signaling. Furthermore, PIEZO1 is predicted to be a downstream target gene of miR-139-5p, and ionizing radiation leads to increased PIEZO1 mRNA and protein expression. We hypothesized that miR-139-5p might regulate PIEZO1 expression to modulate radiation-induced injury in type II alveolar epithelial cells. Therefore, it is of great practical significance to explore new ways to prevent and treat RIPI and break through the existing research bottlenecks for improving the prevention and treatment of RIPI.

Keywords

Ionizing radiation, miR-139-5p, PIEZO1, Radiation-induced pulmonary injury

Introduction

The human lung is a sensitive organ to ionizing radiation.1 Radiation-induced pulmonary injury (RIPI) could happen not only in radiotherapy for chest tumors, but also in practitioners receiving long-term low-dose radiation.2 Approximately 35% of lung and breast cancer patients would develop RIPI after chest radiation therapy.3 RIPI is mainly divided into three stages comprising the asymptomatic phase,4 radiation-induced pneumonitis,5 and radiation-induced lung fibrosis.6,7 When the disease progresses to the pulmonary fibrosis, this would cause not only irreversible damage to the respiratory system and affect the long-term quality of life, but also induce life-threatening respiratory failure.3,7 Hence, finding interventions to delay or reverse the development of RIPI remains a critical issue in current clinical practice. In particular, early therapeutic intervention could significantly improve survival in RIPI.

In addition, type II alveolar epithelial cells play an essential role in lung injury caused by ionizing radiation.3 Extensive type II alveolar epithelial cells would be damaged after radiation stimulated the release of pro-inflammatory cytokines, thus leading to aggravated lung inflammation, increased epithelial and endothelial cell injury, and enhanced proliferation of interstitial cells and interstitial edema.8

PIEZO1 is a mechanically sensitive ion channel discovered in recent years.9 It is expressed in various lung cells according to the epithelial cells (bronchus and alveolus) and endothelial cells.10–12 A recent study has found that cyclical hydrostatic pressure could trigger an inflammatory response by activating the ion channel PIEZO1 in myeloid cells of the lung.13 Similarly, in the progress of RIPI, the damaged mechanical characteristics of the cellular microenvironment could also be sensed by PIEZO1.14

In addition to the change in the activation status of PIEZO1, whether there is a change in the PIEZO1 expression during RIPI is still unclear.

However, there are many studies confirming the relationship between miRNAs and lung injury. In an acute lung injury model, miRNA-1246 could mediate lung inflammation and apoptosis through NF-κB activation and Wnt/β-catenin inhibition.15 One study found that miR-34b-3p, miR-96-5p, and miR-802-5p in C57BL/6 mice lung tissue were associated with TGF-β signaling after whole chest irradiation.16

Compared with normal bronchial epithelial cells, the miR-139-5p had a low expression in lung adenocarcinoma cells.17 Furthermore, the expression of miR-139-5p was significantly decreased after a single 3-Gy dose of irradiation for 4–8 h in breast cancer cells. Reduced miR-139-5p expression was related to radiotherapy resistance of breast cancer cells by upregulating the target genes.18 Several miRNAs predicting databases, such as TargetScanHuman 7.2,19 miRBase,20,21 and ENCORI,22 also predicted that PIEZO1 could be the target gene of miR-139-5p (Fig. 1a–c).23 Nevertheless, whether the impact of miR-139-5p on radiation-induced injury in type II alveolar epithelial cells depends on PIEZO1 signaling is still unknown.

<italic>PIEZO1</italic> could be a target gene of miR-139-5p, and ionizing radiation would lead to increased <italic>PIEZO1</italic> mRNA and protein expression <italic>in vitro</italic>.
Fig. 1  PIEZO1 could be a target gene of miR-139-5p, and ionizing radiation would lead to increased PIEZO1 mRNA and protein expression in vitro.

A-C, The TargetScanHuman 7.2, miRbase, and ENCORI databases predicted that PIEZO1 was a target gene of miR-139-5p. D, 238 genes were intersected in three databases predictions. E and F, Kaplan-Meier Plotter31,32 showed that among breast cancer patients, high miR-139-5p expression and low PIEZO1 expression were associated with longer survival time. The inverse effects of miR-139-5p and PIEZO1 on cancer prognosis were consistent with the prediction that PIEZO1 acted as a target of miR-139-5p. G, The expression of PIEZO1 mRNA in the RLE-6TN cells was increased at 8 h after radiation (4-Gy). N = 3 per group per time point. H, Representative Western blot images showing that the PIEZO1 protein expression was increased at 8 h after radiation. (The bars represent SD. The significance was calculated with the Student’s t-test. *p < 0.05 and ***p < 0.001). RLE-6TN, rat type II alveolar epithelial cells; SD, Standard deviation.

Hypothesis

We hypothesize that during RIPI, PIEZO1 expression is enhanced following reduced expression of miR-139-5p, which would participate in the pathogenesis of RIPI in type II alveolar epithelial cells. Firstly, PIEZO1 would be predicted to be the target gene of miR-139-5p by some miRNAs target gene prediction resources.19 Previous research has proven that ionizing radiation could inhibit the expression of miR-139-5p and upregulate downstream target genes related to radiotherapy resistance.18 We thereby assume that ionizing radiation could upregulate the expression of PIEZO1 by downregulating miR-139-5p. Secondly, it was shown that PIEZO1 could promote HIF-1α accumulation in myeloid cells to regulate innate immunity and bleomycin-induced lung fibrosis.13 Thirdly, HIF-1α was involved in the pathogenesis of radiation-induced pneumonia.24 Thereby, this would seem reasonable to speculate that PIEZO1 could also be activated and aggravate lung inflammation through HIF-1α when type II alveolar epithelial cells were exposed to ionizing radiation (Fig. 2).

A schematic plot illustrating the potential role of miR-139-5p/<italic>PIEZO1</italic> signaling in RIPI.
Fig. 2  A schematic plot illustrating the potential role of miR-139-5p/PIEZO1 signaling in RIPI.

When the alveolar type II epithelial cells were subjected to ionizing radiation, the expression of miR-139-5p was inhibited, thus leading to an increased PIEZO1 expression. The enhanced PIEZO1 expression could stabilize HIF-1α, thereby activating inflammation, damaging the type II epithelial cells as well as endothelial cells, and aggravating RIPI. RIPI, Radiation-induced pulmonary injury.

Statistical analysis

GraphPad software 8.0 (GraphPad Software, Inc., La Jolla, CA, USA) was used to perform the statistical analyses. Data were presented as the mean ± standard deviation (SD). Differences among groups were evaluated by a student’s t-test. Each experiment was repeated as three independent experiments unless specified. p < 0.05 was considered to be statistically significant.

Rationale for the hypothesis

RIPI and miR-139-5p

miR-139-5p was initially identified as a tumor suppressor gene in breast cancer,25 colorectal cancer,26 prostate cancer,27,28 and bladder cancer.29 It was also found to be an effective regulator of the radiotherapy response.30,31 After ionizing radiation, the expression of miR-139-5p was inhibited in breast cancer cells.18 This led to an increased expression of the miR-139-5p target genes related to radiotherapy resistance, such as POLQ, TOP1, TOP2A, and MAT2A.18 Conversely, miR-139-5p mimics have a strong synergistic effect with radiation in vitro and in vivo. Furthermore, miR-139-5p could modulate Notch1 signaling, and an overexpression of miR-139-5p could downregulate the expression of the Notch1 protein that could inhibit an epithelial-mesenchymal transition, which would be a critical process in the development of RIPI.32 Taken together, this evidence would suggest that miR-139-5p might play a role in RIPI.

PIEZO1 might be a downstream target gene of miR-139-5p

We chose the TargetScanHuman 7.2,19 miRBase,20,21 and ENCORI22 miRNA target predicting resources to search for potential miR-139-5p target genes related to RIPI. The above three databases all predicted that PIEZO1 was possibly a target of miR-139-5p, which the intersection of the three databases predictions had 238 genes (Fig. 1d). Two of the three databases displayed a high predictive score in PIEZO1, a target score of 82 in miRBase and 99 context++ score percentile in TargetScanHuman 7.2. More interestingly, the impacts of miR-139-5p and PIEZO1 expression on the survival of patients with breast cancer were contrasting.23 It was shown that a high expression of miR-139-5p (Fig. 1e) or low expression of PIEZO1 (Fig. 1f) was related to longer survival time in breast cancer patients. The inverse effects of miR-139-5p and PIEZO1 on the breast cancer prognosis were consistent with the prediction that miR-139-5p negatively regulated the PIEZO1 expression made by the miRNA target predicting databases.

PIEZO1 in type II alveolar epithelial cells

As a mechanically sensitive ion channel, PIEZO1 would be crucial for the generation of mechanically gated non-selective cation current and would play an important role in the process of mechanical transduction.33 Previous studies and data from the BioGPS database (http://biogps.org/ ) demonstrated that PIEZO1 was widely expressed in various kinds of lung (fetal lung and lung) cells, such as bronchial epithelial cells, lung endothelial cells,12,34 alveolar epithelial cells (types I and II), and so on.10,11 In the pulmonary endothelial cells, PIEZO1 was related to angiogenesis, hydrostatic pressure-induced pulmonary edema, and ventilator-induced lung injury.35,36 In the lung myeloid cells, PIEZO1 promoted HIF-1α stabilization to trigger inflammation under stress.13 In alveolar epithelial cells, mechanical stress during the respiratory cycle activated PIEZO1, consequently causing a Ca2+ influx, thereby releasing an alveolar surfactant.37 However, there has been no research to prove that the PIEZO1 protein in the alveolar epithelial cells could regulate RIPI.

PIEZO1 with RIPI

Radiation-induced lung injury includes acute radiation-induced pneumonia and chronic pulmonary fibrosis.5 In the process of pulmonary injury caused by ionizing radiation, the structure and composition of the extracellular matrix would be damaged, thus leading to high levels of stress and strain throughout the lung.14PIEZO1 could be activated due to changes in the mechanics of the cellular microenvironment.13 Our ongoing study using the rat type II alveolar epithelial cells (RLE-6TN) found that a single dose of 4-Gy radiation after 8 h increased both the mRNA and protein levels of PEIZO1 (Fig. 1g, h). The previous study demonstrated that PIEZO1 induced the EDN1 expression through the Ca2+ influx to drive the HIF-1α accumulation and inflammation in the lung myeloid cells.13 Simultaneously, HIF-1α signaling was found to play an essential role in RIPI.5 During RIPI, the type II alveolar epithelial cells could also act as a source of inflammation.3 Therefore, we further speculated that PIEZO1 could aggravate lung inflammation after radiation by regulating the HIF-1α signaling in type II alveolar epithelial cells.

Verification of the hypothesis and clinical implications

We proposed a novel mechanism whereby miR-139-5p would regulate PIEZO1/HIF-1α signaling to modulate ionizing radiation-induced pulmonary injury. PIEZO1 in type II alveolar epithelial cells could act as a potential target in protecting the lung from ionizing radiation injury. This hypothesis would be verified by a series of experiments. Firstly, we would confirm that the decreased expression of miR-139-5p would be accompanied with an increased PIEZO1 expression after ionizing radiation. Secondly, the miRNA/target gene relationship between miR-139-5p and PIEZO1 would be validated by a series of experiments. Thirdly, the roles of miR-139-5p and PIEZO1 in inflammation activated by the type II alveolar epithelial cells after radiation will be examined. Finally, an investigation would be undertaken to examine whether the impact of miR-139-5p on RIPI was dependent on PIEZO1/HIF-1α signaling.

Future directions

We further tend to conduct a study to confirm whether PIEZO1 could be modulated by miR-139-5p and would explore the specific mechanisms through molecular biology experiments.

Conclusions

Ionizing radiation inhibits miR-139-5p expression. PIEZO1 is a predicted downstream target of miR-139-5p. After radiation, the upregulation of the PIEZO1 expression could aggravate inflammation and promote the development of RIPI in type II alveolar epithelial cells. Therefore, well-designed experiments would be needed to verify this hypothesis.

Abbreviations

RIPI: 

Radiation-induced pulmonary injury

RLE-6TN: 

rat type II alveolar epithelial cells

Declarations

Acknowledgement

We thank the Department of Nuclear Radiation Injury and Monitoring for the technical assistance.

Funding

The authors did not receive any financial support for this publication.

Conflict of interest

All authors have no interests to declare.

Authors’ contributions

JH and YL collected the data and wrote the manuscript. XG and XL performed all the bioinformatic analyses. YL offered conceptual insight. YL and HZ suggested intellectual support and supervised the project, and SW helped interpret the work. HZ, XL, and SW participated in the design of the study.

References

  1. Ding NH, Li JJ, Sun LQ. Molecular mechanisms and treatment of radiation-induced lung fibrosis. Curr Drug Targets 2013;14(11):1347-1356 View Article PubMed/NCBI
  2. World Health Organization. Available from: https://www.who.int/news-room/fact-sheets/detail/ionizing-radiation-health-effects-and-protective-measures. Accessed October 13, 2020 View Article PubMed/NCBI
  3. Chen Z, Wu Z, Ning W. Advances in Molecular Mechanisms and Treatment of Radiation-Induced Pulmonary Fibrosis. Translational Oncology 2019;12(1):162-169 View Article PubMed/NCBI
  4. He Y, Thummuri D, Zheng G, Okunieff P, Citrin DE, Vujaskovic Z, et al. Cellular senescence and radiation-induced pulmonary fibrosis. Transl Res 2019;209:14-21 View Article PubMed/NCBI
  5. Hanania AN, Mainwaring W, Ghebre YT, Hanania NA, Ludwig M. Radiation-Induced Lung Injury: Assessment and Management. Chest 2019;156(1):150-162 View Article PubMed/NCBI
  6. Chen Z, Wu Z, Ning W. Advances in Molecular Mechanisms and Treatment of Radiation-Induced Pulmonary Fibrosis. Transl Oncol 2019;12(1):162-169 View Article PubMed/NCBI
  7. Graves PR, Siddiqui F, Anscher MS, Movsas B. Radiation pulmonary toxicity: from mechanisms to management. Semin Radiat Oncol 2010;20(3):201-207 View Article PubMed/NCBI
  8. Siva S, MacManus M, Kron T, Best N, Smith J, Lobachevsky P, et al. A pattern of early radiation-induced inflammatory cytokine expression is associated with lung toxicity in patients with non-small cell lung cancer. PLoS One 2014;9(10):e109560 View Article PubMed/NCBI
  9. Gottlieb PA, Sachs F. Piezo1: properties of a cation selective mechanical channel. Channels (Austin) 2012;6(4):214-219 View Article PubMed/NCBI
  10. Bhattacharya J, Hough RF. Piezo1 in the Lung: At Last!. Am J Respir Cell Mol Biol 2019;60(6):609-610 View Article PubMed/NCBI
  11. Stewart TA, Davis FM. Formation and Function of Mammalian Epithelia: Roles for Mechanosensitive PIEZO1 Ion Channels. Front Cell Dev Biol 2019;7:260 View Article PubMed/NCBI
  12. Friedrich E, Hong Z, Xiong S, Zhong M, Di A, Rehman J, et al. Endothelial cell Piezo1 mediates pressure-induced lung vascular hyperpermeability via disruption of adherens junctions. Proc Natl Acad Sci U S A 2019;116(26):12980-12985 View Article PubMed/NCBI
  13. Solis AG, Bielecki P, Steach HR, Sharma L, Harman CCD, Yun S, et al. Mechanosensation of cyclical force by PIEZO1 is essential for innate immunity. Nature 2019;573(7772):69-74 View Article PubMed/NCBI
  14. Waters CM, Roan E, Navajas D. Mechanobiology in lung epithelial cells: measurements, perturbations, and responses. Compr Physiol 2012;2(1):1-29 View Article PubMed/NCBI
  15. Suo T, Chen GZ, Huang Y, Zhao KC, Wang T, Hu K. miRNA-1246 suppresses acute lung injury-induced inflammation and apoptosis via the NF-kappaB and Wnt/beta-catenin signal pathways. Biomed Pharmacother 2018;108:783-791 View Article PubMed/NCBI
  16. Rogers CJ, Lukaszewicz AI, Yamada-Hanff J, Micewicz ED, Ratikan JA, Starbird MA, et al. Identification of miRNA signatures associated with radiation-induced late lung injury in mice. PLoS One 2020;15(5):e0232411 View Article PubMed/NCBI
  17. Li J, He X, Wu X, Liu X, Huang Y, Gong Y, et al. miR-139-5p Inhibits Lung Adenocarcinoma Cell Proliferation, Migration, and Invasion by Targeting MAD2L1. Computational and Mathematical Methods in Medicine 2020;2020:1-10 View Article PubMed/NCBI
  18. Pajic M, Froio D, Daly S, Doculara L, Millar E, Graham PH, et al. miR-139-5p Modulates Radiotherapy Resistance in Breast Cancer by Repressing Multiple Gene Networks of DNA Repair and ROS Defense. Cancer Res 2018;78(2):501-515 View Article PubMed/NCBI
  19. Agarwal V, Bell GW, Nam JW, Bartel DP. Predicting effective microRNA target sites in mammalian mRNAs. Elife 2015;4:e05005 View Article PubMed/NCBI
  20. Liu W, Wang X. Prediction of functional microRNA targets by integrative modeling of microRNA binding and target expression data. Genome Biology 2019;20(1):18 View Article PubMed/NCBI
  21. Chen Y, Wang X. miRDB: an online database for prediction of functional microRNA targets. Nucleic Acids Res 2020;48:D127-D131 View Article PubMed/NCBI
  22. Li J, Liu S, Zhou H, Qu L, Yang J. starBase v2.0: decoding miRNA-ceRNA, miRNA-ncRNA and protein-RNA interaction networks from large-scale CLIP-Seq data. Nucleic Acids Res 2014;42:D92-97 View Article PubMed/NCBI
  23. Györffy B, Lanczky A, Eklund A, Denkert C, Budczies J, Li Q, et al. An online survival analysis tool to rapidly assess the effect of 22,277 genes on breast cancer prognosis using microarray data of 1,809 patients. Breast Cancer Res Treat 2010;123(3):725-731 View Article PubMed/NCBI
  24. Jin H, Yoo Y, Kim Y, Kim Y, Cho J, Lee YS. Radiation-Induced Lung Fibrosis: Preclinical Animal Models and Therapeutic Strategies. Cancers (Basel) 2020;12(6):1561 View Article PubMed/NCBI
  25. Adhami M, Haghdoost AA, Sadeghi B, Malekpour Afshar R. Candidate miRNAs in human breast cancer biomarkers: a systematic review. Breast Cancer 2018;25(2):198-205 View Article PubMed/NCBI
  26. Shen K, Mao R, Ma L, Li Y, Qiu Y, Cui D, et al. Post-transcriptional regulation of the tumor suppressor miR-139-5p and a network of miR-139-5p-mediated mRNA interactions in colorectal cancer. FEBS J 2014;281(16):3609-3624 View Article PubMed/NCBI
  27. Song CJ, Chen H, Chen LZ, Ru GM, Guo JJ, Ding QN. The potential of microRNAs as human prostate cancer biomarkers: A meta-analysis of related studies. J Cell Biochem 2018;119(3):2763-2786 View Article PubMed/NCBI
  28. Nam RK, Benatar T, Wallis CJD, Kobylecky E, Amemiya Y, Sherman C, et al. MicroRNA-139 is a predictor of prostate cancer recurrence and inhibits growth and migration of prostate cancer cells through cell cycle arrest and targeting IGF1R and AXL. Prostate 2019;79(12):1422-1438 View Article PubMed/NCBI
  29. Chi Q, Wang ZY, Li HY, Song DB, Xu H, Ma G, et al. Tumor-suppressor microRNA-139-5p restrains bladder cancer cell line ECV-304 properties via targeting Connexin 43. Chin Med J (Engl) 2019;132(19):2354-2361 View Article PubMed/NCBI
  30. Buffa FM, Camps C, Winchester L, Snell CE, Gee HE, Sheldon H, et al. microRNA-associated progression pathways and potential therapeutic targets identified by integrated mRNA and microRNA expression profiling in breast cancer. Cancer Res 2011;71(17):5635-5645 View Article PubMed/NCBI
  31. Curtis C, Shah SP, Chin SF, Turashvili G, Rueda OM, Dunning MJ, et al. The genomic and transcriptomic architecture of 2,000 breast tumours reveals novel subgroups. Nature 2012;486(7403):346-352 View Article PubMed/NCBI
  32. Wang Y, Zhong Y, Zhang C, Liao J, Wang G. PM2.5 downregulates MicroRNA-139-5p and induces EMT in Bronchiolar Epithelium Cells by targeting Notch1. J Cancer 2020;11(19):5758-5767 View Article PubMed/NCBI
  33. Coste B, Mathur J, Schmidt M, Earley TJ, Ranade S, Petrus MJ, et al. Piezo1 and Piezo2 are essential components of distinct mechanically activated cation channels. Science 2010;330(6000):55-60 View Article PubMed/NCBI
  34. Zhong M, Wu W, Kang H, Hong Z, Xiong S, Gao X, et al. Alveolar Stretch Activation of Endothelial Piezo1 Protects Adherens Junctions and Lung Vascular Barrier. Am J Respir Cell Mol Biol 2020;62(2):168-177 View Article PubMed/NCBI
  35. Zhong M, Wu W, Kang H, Hong Z, Xiong S, Gao X, et al. Alveolar Stretch Activation of Endothelial Piezo1 Protects Adherens Junctions and Lung Vascular Barrier. Am J Respir Cell Mol Biol 2020;62(2):168-177 View Article PubMed/NCBI
  36. Friedrich EE, Hong Z, Xiong S, Zhong M, Di A, Rehman J, et al. Endothelial cell Piezo1 mediates pressure-induced lung vascular hyperpermeability via disruption of adherens junctions. Proc Natl Acad Sci U S A 2019;116(26):12980-12985 View Article PubMed/NCBI
  37. Diem K, Fauler M, Fois G, Hellmann A, Winokurow N, Schumacher S, et al. Mechanical stretch activates piezo1 in caveolae of alveolar type I cells to trigger ATP release and paracrine stimulation of surfactant secretion from alveolar type II cells. FASEB J 2020;34(9):12785s2804 View Article PubMed/NCBI