Investigating the effect of Banxia Houpu decoction for the treatment of gastrointestinal dysfunction after multiple fractures: involvement of interstitial cells of Cajal

Yu Zhang^1,2,
Wei-Qiang Zhao¹,
Meng-Qiang Fan¹,
Jian-Sheng Qian³,
Hui-Hui Xu⁴,
Yang Zheng^1,4,
Jiang Hua^1,4,*,
Li-Pei Wang^5,* and
Jie-Feng Huang^1,4,*

Gastroenterology & Hepatology Research 2023;5(4):18

doi: 10.53388/ghr2023-03-082

Published online: December 30, 2023

Author information

Citation: Zhang Y, Zhao WQ, Fan MQ, Qian JS, Xu HH, Zheng Y, et al. Investigating the effect of Banxia Houpu decoction for the treatment of gastrointestinal dysfunction after multiple fractures: involvement of interstitial cells of Cajal. Gastroenterol & Hepatol Res. 2023;5(4):18. doi: 10.53388/ghr2023-03-082.

Abstract

Background

Fracture is a common large-organ traumatic injury and usually leads to complications in other systems. Multiple fractures are often associated with gastrointestinal (GI) dysfunction. However, no study has evaluated the treatment of GI dysfunction. The purpose of the study is to explore the efficacy and mechanism based on ICC pathway of Banxia-Houpu decoction on GI.

Methods

Male Sprague-Dawley mice were randomly divided according to the fracture modeling method and intervention. We detected the "GI residual rate" and motility. Gastric antrum and jejunum tissues were dissected for hematoxylin and eosin staining to observe the structural integrity of the GI tract, and immunohistochemistry was used to detect the expression of c-kit protein.

Results

Compared with the fracture group the GI residual rates in the Banxia Houpu decoction and mosapride groups were significantly low, while GI motility was significantly high. HE staining revealed significant GI tissue necrosis and inflammatory cell infiltrations in the fracture groups. The Banxia Houpu decoction and mosapride groups, exhibited less pathology. Immunohistochemical staining showed upregulated c-kit protein expression in the fracture groups. The c-kit protein level was decreased in the mosapride group. Additionally, c-kit protein expression in the Banxia Houpu decoction groups was significantly decreased in a concentration-dependent manner.

Conclusion

Banxia Houpu decoction improves GI dysfunction after multiple fractures, reduces inflammation and necrosis of gastric epithelial cells, and inhibits c-kit protein expression in GI tissues of mice. Results showed revealed one of the mechanisms underlying the effects of this decoction on the GI dysfunction in mice after multiple fractures.

Keywords

Banxia Houpu Decoction, C-kit, Gastrointestinal Diseases, Fractures, Multiple, Models, Animal

Copyright © 2025 Authors. This is an Open Access article distributed under the terms of the Creative Commons Attribution-Noncommercial 4.0 License (CC BY-NC 4.0), permitting all non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Zhang Y, Zhao WQ, Fan MQ, Qian JS, Xu HH, Zheng Y, et al. Investigating the effect of Banxia Houpu decoction for the treatment of gastrointestinal dysfunction after multiple fractures: involvement of interstitial cells of Cajal. Gastroenterol & Hepatol Res. 2023;5(4):18. doi: 10.53388/ghr2023-03-082.

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Article History

Received	Revised	Accepted	Published
			December 30, 2023

DOI http://dx.doi.org/10.53388/ghr2023-03-082

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