v
Search
Advanced Search

Publications > Journals > Gene Expression > Article Full Text

  • Review Article
  • OPEN ACCESS

Acetaldehyde Dehydrogenases in Liver Zonation and Liver Cancer

  • Brady Jin-Smith,
  • Natacha Jn-Simon,
  • Sreenivasulu Basha,
  • Chunbao Sun,
  • Shang Wu,
  • Joshua Max Barkin and
  • Liya Pi* 
 Author information  Cite
Gene Expression   2023;22(1):66-76

doi: 10.14218/GE.2022.00022

Abstract

The liver maintains important homeostatic functions such as metabolism and detoxification. Failure to remove toxic intermediates can cause hepatic damage, liver fibrosis, and even cancer development. This review focuses on acetaldehyde dehydrogenases (ALDHs), a group of key enzymes within the ALDH superfamily with the ability to convert highly reactive aldehyde substrates to the corresponding carboxylic acids in NAD(P)-dependent manners. These enzymes participate in a diverse array of biological processes such as detoxification, biosynthesis, antioxidant, and regulatory functions. ALDH dysfunction can disrupt homeostasis, leading to toxic buildup, tissue damage, and cancer. Here, we examine the expression patterns of hepatic ALDHs in adult normal human livers and two types of liver cancers—hepatocellular carcinoma and cholangiocarcinoma. We also investigate their distributions in relation to liver zonation. These observations provide deep insights into previously unrecognized spatial and temporal regulation of ALDHs in liver zonation.

Keywords

ALDH, Lipid peroxidation, Liver cancer, Hepatocellular carcinoma, Cholangiocarcinoma

Introduction

The liver is composed of many small functional units known as liver lobules. Hepatocytes, bile ductular epithelial cells, hepatic stellate cells (HSCs), Kupffer cells, and sinusoidal endothelial cells reside in the liver lobules and participate in homeostasis. Hepatocytes are the major functional cells, and they are stacked one by one in hepatic cords radiating from the central veins to the portal triad. Segregation of hepatocytes into different metabolic zones with functions adapted to oxygen and nutrients occurs according to their concentration nutrient gradients from high to low along the blood flow. Due to endogenous or exogenous exposure during substance exchange and metabolism, toxic intermediates can accumulate in the liver, which filters the blood by removing potentially harmful substances using detoxification mechanisms. Failure to remove these toxins can cause hepatotoxicity. Hepatocyte damage can occur by diverse hepatic insults ranging from viral infections to metabolic syndromes, obesity, drug toxicity, and alcohol abuse. Chronic incidence of these pathological conditions recruits inflammatory cells and activates nonparenchymal cells such as HSCs, leading to liver scarring, cirrhosis, and even liver failure. Liver cancers such as hepatocellular carcinoma (HCC) and cholangiocarcinoma (CCA) may eventually develop due to the profibrotic microenvironments, resulting in a life-threatening condition. Therefore, characterizing detoxification enzymes in the liver has therapeutic potential to reduce hepatic damage and prevent liver injury and cancer development. This review focuses on acetaldehyde dehydrogenases (ALDHs), a group of key enzymes that catalyze the irreversible oxidation of various aliphatic and aromatic aldehydes to the corresponding carboxylic acids. The distribution patterns of these detoxification genes in normal adult livers, liver zonation, HCC, and CCA were also compared using publicly available databases.

ALDHs and their functions

ALDHs consist of 24 families in the eukaryotic ALDH gene superfamily. Nineteen of them are found in the human genome and belong to the ALDH1–9, ALDH16, and ALDH18 families.1 There are six isotype genes in the ALDH1 family (ALDH1A1, ALDH1A2, ALDH1A3, ALDH1B1, ALDH1L1, and ALDH1L2). Among them, ALDH1A1, ALDH1A2, and ALDH1A3 encode cytosolic enzymes that oxidize retinal and aliphatic aldehydes. ALDH1A1 protein binds to retinaldehyde in great affinity and has been considered a major retinoid acid-metabolizing enzyme.2 Cytosolic ALDH1A1 also plays a role in acetaldehyde oxidation and alcohol preference by mediating the gamma-aminobutyric acid synthesis pathway.3 In the liver, ALDH1A1 has been shown to be a novel determinant of gluconeogenesis and lipid metabolism independent of adiposity.4 Deletion of the mouse Aldh1a1 gene significantly attenuates hepatic triacylglycerol synthesis by increasing adenosine monophosphate (AMP)-activated protein kinase alpha activity and decreasing the expression of lipogenic targets of AMP-activated protein kinase alpha. The ALDH1 family also contains a mitochondrial ALDH1B1 enzyme involved in metabolizing both retinal and acetaldehyde. It has a high affinity to acetaldehyde only secondary to ALDH2 and catalyzes various aldehyde substrates of acetaldehyde and derivatives of lipid peroxidation.5 ALDH1L1 and ALDH1L2 are other members of the ALDH1 family that can metabolize 10-formyltetrahydrofolate. They are in the mitochondria and cytosol, respectively.

ALDH2 is the only member of the ALDH2 family. This mitochondrial enzyme is primarily responsible for the oxidization of the majority of hepatic acetaldehyde in vivo;6 however, ALDH1A1 and ALDH1B1 also have a detectable affinity to acetaldehyde.7 The ALDH3 family consists of three endoplasmic reticulum-located enzymes (ALDH3A2, ALDH3B1, and ALDH3B2) and one cytosolic enzyme (ALDH3A1) that is also partially distributed in the nucleus. ALDH3A1 uses aromatic and aliphatic aldehydes as substrates. ALDH3A2 converts fatty aldehydes to fatty acids, while ALDH3B1 mainly oxidizes octanal. It has been reported that the ALDH3 family has a specific substrate spectrum for all members,8 although substrates for ALDH3B2 presently remain unknown.

ALDH4A1, ALDH5A1, and ALDH6A1 are found in mitochondria and can metabolize glutamate-gamma-semialdehyde, succinate semialdehyde, and malonate semialdehyde, respectively.9,10 ALDH7A1, located in the cytosol, is responsible for the oxidation of alpha-aminoadipic semialdehyde.11 Like ALDH7A1, ALDH8A1 is found in the cytosol but is involved with a cytosolic enzyme for retinal metabolism and the kynurenine pathway for tryptophan catabolism.12 Additionally, ALDH9A1 is also located in the cytosol and metabolizes gamma-aminobutyraldehyde. ALDH16A1 is a transmembrane protein, but its substrate is still unknown. ALDH18A1 is a mitochondrial enzyme and shares similar substrates with ALDH4A1 for metabolizing glutamic gamma-semialdehyde. Most of the ALDH gene families have the cysteine (PS00070) and glutamic acid (PS00687) active site, but ALDH18A1 encodes a bifunctional protein with a glutamate 5-kinase (PS00902) at the N-terminal site and a gamma-glutamyl phosphate reductase (PS01223) at the C-terminal site.13 Therefore, there is a distal evolutionary connection between ALDH18A1 and other ALDHs.

Pivotal roles of ALDHs also have been documented based on human genetic disorders. Mutations of ALDH1A2 protein at residue 151 from alanine to serine (A151S) or at residue 157 from isoleucine to threonine (I157T) cause congenital heart disease.14 ALDH1A3 protein with an arginine mutation at residue 89 to cysteine (R89C) is linked to autosomal recessive anophthalmia and microphthalmia, which are rare developmental eye defects occurring in early fetal development.15 The ALDH1B1 mutant with alanine to valine at position 86 (A86V) is associated with alcohol-induced hypersensitivity.16,17 A mutation at residue 793 (D793G) in ALDH1L1 protein is correlated with Hodgkin’s lymphoma.18 The mutation at position 504 from glutamic acid to lysine (E504K) in ALDH2 protein is a risk factor for esophageal cancer,19,20 diabetic cardiomyopathy,21–23 cardiac dysfunction,24 Alzheimer’s disease,25 and colorectal cancer.26,27 The ALDH3A2 mutation at residue 266 from lysine to asparagine (K266N) causes an inherited neurocutaneous disorder known as Sjögren–Larsson syndrome.28 ALDH4A1 protein with a mutation at residue 352 from serine to leucine (S352L) is correlated with hyperprolinemia type 2, an autosomal recessive disorder of proline metabolism.29 ALDH5A1 with a mutation at position 301 from lysine to glutamic acid (K301E) disrupts the normal degradation of gamma-hydroxybutyric acid, resulting in a rare metabolic disorder known as gamma-hydroxybutyric aciduria, which is characterized by a highly heterogeneous neurological phenotype ranging from mild to very severe.30 Substitutions at position 535 from arginine to cysteine (R535C) or position 466 from glycine to arginine (G466R) in ALDH6A1 are associated with demyelination and transient methylmalonic aciduria.31 Three mutations in ALDH7A1, which include leucine to proline at position 455 (L455P), glutamic acid to glutamine at position 427 (E427Q), and asparagine to leucine at position 301 (N301I), are associated with pyridoxine-dependent epilepsy and folic acid-responsive seizures.31 The ALDH16A1 mutation from proline to arginine (P527R) causes gout and mast syndrome.32 ALDH18A1 with a mutation from arginine to glutamine at position 84 (R84Q) results in urea cycle defects characterized by hyperprolinemia, hypoornithinemia, hypocitrullinemia, hypoargininemia, and hyperammonemia.33

Furthermore, ALDH enzymes are involved in many vital physiological processes. By binding to substrates for endobiotic and xenobiotic functions, they not only detoxify potentially hazardous aldehydes, but they also mediate antioxidant activities through direct (glutathione-like) and indirect (generating NAD(P)H) actions. Some of them can transform vitamin A into retinoic acid and perform osmoregulatory functions. Moreover, ALDHs can also protect cells against lipid aldehydes in environments with high levels of oxidative stress. One negative implication of this protective activity is that it allows cancer stem cells or other tumor cells to escape drug toxicity, thus causing cancer resistance.

Cell-type expression patterns of ALDHs in the human adult liver

The liver consists of multiple types of cells. About 80% of liver cells are hepatocytes, which maintain the central liver functions of metabolism, biosynthesis, and detoxification. Bile ductular epithelial cells are the other type of parenchymal cells in the liver, and they form bile ducts to carry out bile acid drainage. Vascular endothelial cells lining the blood vessel walls form sinusoids. HSCs are typically vitamin A-storing cells in the space of Disse between the sinusoid and hepatic plates. The residual macrophage cells in the liver are known as Kupffer cells. They are located near the blood vessel walls in sinusoids as part of immune surveillance. Blood cells, including T cells, B cells, and erythroid cells, are also rich in the liver. To examine the expression patterns of ALDHs in human adult livers, we took advantage of the public database Human Protein Altas (https://www.proteinatlas.org ) and extracted the single-cell expression data of all ALDHs except ALDH1A7 and ALDH3B2. Transcript profiling in this database was based on a combination of two transcriptomics datasets (Human Protein Atlas and Genotype-Tissue Expression) that correspond to a total of 14,590 samples from 54 different human normal tissue types, according to Fagerberg et al.34 As shown in Figure 1, hepatocytes are the main cellular source for 12 ALDH genes (ALDH1A1, ALDH1B1, ALDH2, ALDH1L1, ALDH9A1, ALDH8A1, ALDH5A1, ALDH6A1, ALDH3A1, ALDH3A2, ALDH7A1, and ALDH4A1). Although ALDH18A1 and ALDH9A1 are highly expressed in hepatocytes, these two genes were also detectable in almost all other cell types (B cells, erythroid cells, T cells, bile ductular epithelial cells, endothelial cells, Kupffer cells, and HSCs) in adult human livers. ALDH16A1 is another gene with wide expression across all cell types in the liver. However, B cells and Kupffer cells have relatively higher levels of ALDH16A1 than hepatocytes. Notably, some ALDHs are not expressed in hepatocytes. For example, ALDH3B1 is predominantly found in Kupffer cells, while ALDH1A2 and ALDH1L2 only have been detected at low levels in B cells, and low levels of ALDH1A3 have been found in HSCs and bile endothelial progenitor cells. Such differential expression profiles indicate that hepatocytes utilize the majority of ALDHs, whereas other cell types may also use specific enzymes for unique needs during liver homeostasis.

Expression patterns of <italic>ALDHs</italic> in cells within the human adult liver.
Fig. 1  Expression patterns of ALDHs in cells within the human adult liver.

Data were extracted based on the maximal transcripts per million (nTPM) for each cell type from the Human Protein Atlas (https://www.proteinatlas.org ). ALDH, acetaldehyde dehydrogenase; EC, vascular endothelial cells; HSC, hepatic stellate cell.

ALDH expression patterns in relation to murine liver zonation

The mammalian liver consists of repeating hexagonally shaped lobules as functional units. As shown in Figure 2a, each liver lobule consists of around 9–12 concentric layers of hepatocytes in mice.35,36 Liver zonation refers to the phenomenon of spatial and temporal segregation of hepatocytes according to their distinct functions in hepatic cords. Single-molecule fluorescence in-situ hybridization can provide sensitivity and dynamic ranges for precise measurement of the mRNA content of hepatocytes in mammalian livers.37 Combining this technique with single-cell RNA sequencing has revealed the entire transcriptome of thousands of mouse liver cells.36 In this genome-wide reconstruction of liver zonation, nine layers, starting from the central vein to the portal triads, have been designed to determine the global division of labor in the mammalian liver based on lobule coordinates and zonation landmark genes.36 Using this strategy, a probabilistic inference algorithm has been developed to compute the likelihood that each cell belongs to any of these layers according to six landmark genes, including the pericentral genes Glul and Cyp2e19 and the periportal genes Ass110, Asl10, Alb8, and Cyp2f29.36 This reconstruction accuracy is strongly dependent on the extent of zonation of tested landmark genes and only weakly dependent on the intralayer cell-to-cell variability. The precision of reconstructed zonation profiles has been validated using single-molecule fluorescence in-situ hybridization on 20 genes with diverse profiles and has displayed an excellent overall correspondence between the predicted and measured profiles.36

Expression patterns of <italic>Aldhs</italic> in liver zonation.
Fig. 2  Expression patterns of Aldhs in liver zonation.

(a) A cartoon showing the hepatic architecture with layers of hepatic cords, central veins (CV), and portal triads (PT) that contain portal veins, hepatic arteries, and bile ducts. EC: vascular endothelial cells; HSC: hepatic stellate cells. (b) Overview of a liver lobule related to nine layers of hepatocytes in analyses of spatial transcriptomics according to Halpern et al.36 (c) The immunofluorescent staining detects periportal hepatocytes by the Arg1 antibody (green signal), pericentral hepatocytes by the glutamine synthetase (Gs) antibody (red signal), and large hepatic vasculatures by an alpha-smooth muscle actin antibody (yellow signal). The white dashed lines show areas of a liver lobule consisting of 6 PT in the periphery and one CV in the middle. Scar bar: 100 mm. (d) Six types of zonation patterns are found in Aldhs. (e) and (f) Immunohistochemistry showed pericentral patterns of Aldh1a1 protein in normal and damaged livers that were exposed to 5% ethanol/binge in chronic and acute liver injury. Magnification: 200×. ALDH, acetaldehyde dehydrogenase.

The enzyme arginase 1 (Arg1) is involved in the urea cycle, which is a series of reactions that occur in liver cells near periportal zones. The urea cycle processes excess nitrogen, which is generated when proteins and their building blocks (amino acids) are used by the body. The Glul gene product, glutamine synthetase, has opposite patterns that are exclusively located in the first one to two layers of pericentral hepatocytes compared to Arg1. Figure 2b demonstrates the distribution of the periportal enzyme Arg1; the pericentral enzyme glutamine synthetase, which is encoded by Glul; and the perivascular cell marker smooth muscle actin in murine livers. The white dashed lines in Figure 2b indicate hexagon-shaped lobules that are radially polarized to form liver zonation. Considering that key liver genes have been shown to be differentially expressed in different layers of hepatocytes along the liver lobule axis, we examined the distribution of the Aldh gene in normal mouse adult livers using extracted data describing the detailed genome-wide reconstruction of the spatial division of hepatocytes in liver zonation.36 The Aldh gene levels were obtained from supplementary table36 in the zonation matrix for spatial transcriptomics according to Halpern and illustrated by us in heatmaps as shown in Figure 2c–d. These heatmaps for Aldh genes were generated based on average values from layer 1 to layer 9 (Fig. 2c–d). We found six different patterns of these genes in the mouse liver zonation, whereas the Aldh18a1, Aldh1a2, Aldh1a3, Aldh3a1, and Aldh3b2 genes were undetectable and were excluded in the analyses. The first pattern showed a peak increase in the pericentral zones. For example, the Aldh1a1 levels averaged 6.5584E−4 at layer 1 (near the pericentral zone) and 2.3445E−4 at layer 9 (near the periportal zone), showing a roughly 2.797-fold higher level in the pericentral zones than in the periportal zones for the Aldh1a1 gene. The average Aldh2 level was 2.1123E−3 at layer 1 and 1.0253E−3 at layer 9, indicating a 2.06-fold higher level of Aldh2 expression in the pericentral zones versus the periportal zones. Aldh3a2 expression averaged about 1.1139E−3 at layer 1 and dropped to 4.2813E−05 at layer 9, indicating a 26-fold higher level of Aldh3a2 expression in the pericentral zones than in the periportal zones. Aldh16a1 had an average expression of about 8.9078E−05 at layer 1 and 7.1063E−05 at layer 9, a 1.25-fold higher level in Aldh16a1 in the pericentral zones compared to the periportal zones.

The second pattern of ALDHs showed peak expression at the periportal zones. Aldh1b1 expression averaged about 3.78886E−06 at layer 1 and 16685E−05 at layer 9, indicating a 24.19-fold increase in the periportal zones compared to the pericentral zones. Aldh1a7 showed a 1.44-fold increase in the periportal zones compared to the pericentral zones based on its average expression of about 5.91444E−05 at layer 1 and 8.52498E−05 at layer 9. Aldh9a1 had an average expression of about 1.6432E−4 at layer 1 and 2.3246E−4 at layer 9, demonstrating a 1.415-fold periportal elevation. Lastly, Aldh1l1 roughly displayed a 1.66-fold periportal increase, with an average expression of about 5.93E−4 at layer 1 and 9.8656E−4 at layer 9.

The third pattern exhibited peak expression in the middle zones with increased levels in the periportal zones. Aldh1l2 had the highest level in layer 3 (2.149E−07), which was 1150-fold higher than that at layer 1 and 4.28-fold higher than that at layer 9. The fourth pattern showed the lowest expression in the middle zones. Aldh6a1 had the lowest expression (5.61E−05) in layer 3, which was about a 1.43-fold reduction from layer 1 and a 1.30-fold decrease from layer 9. The fifth pattern had peak expression in the middle zone. Aldh8a1 expression averaged about 2.0291E−4 at layer 1 and 2.6596E−4 at layer 9, with the peak average expression of about 2.933E−4 found at layer 7. The sixth pattern revealed two expression peaks located in the middle zones. Aldh1l2 had the highest level in layer 3 (2.149E−07), which was 1150-fold higher than that at layer 1 and 4.28-fold higher than that at layer 9, and another peak expression was at layer 7 (1.18452E−07), which was 633.986-fold higher than that at layer 1 and 2.36-fold higher than that at layer 9.

Spatial sorting enables comprehensive characterization of liver zonation.38 Transcription dynamics in a physiological process indicate that β-catenin signaling directs liver metabolic zonation.39ALDH3A1 expression is not detected in any layers, as indicated in supplementary table by Halpern,36 but overexpression of this gene has been reported in HCC with the Wnt/β-catenin pathway.40 Considering that the Wnt/β-catenin pathway controls pericentral genes, this regulation of ALDH3A1 by Wnt/β-catenin suggests that this enzyme likely is induced by pericentral genes during HCC development. In addition, ALDH1A1 can be regulated by the Wnt/β-catenin pathway.41 It is easy to speculate that the pericentral localization of this gene results from the regulation by the Wnt/β-catenin pathway in normal mouse livers. Our recent data have demonstrated potential regulation of the mouse Aldh1a1 by Yes-associated protein during alcohol-related hepatocyte damage.42 Moreover, we found Aldh1a1 localization in the pericentral zones in normal mouse livers (Fig. 2e). When mice were exposed to a 5% ethanol-containing Liber Dicarli liquid diet for 10 days followed by a binge (5 mg/g body weight), according to Dr. Bin Gao’s National Institute on Alcohol Abuse and Alcoholism model,43 we observed increased staining of Aldh1a1 in the pericentral zones of the ethanol-damaged livers (Fig. 2f). It is conceivable that both the Yes-associated protein and Wnt/β-catenin pathways are involved in regulating Aldh1a1 during alcoholic liver disease.

ALDHs in HCC

HCC is the most frequently diagnosed type of liver cancer with a poor prognosis and no effective treatments. Surveillance Epidemiology End Results have reported that HCC is the fastest-growing cause of cancer-related deaths in the United States since the early 2000s.44,45 To understand the expression patterns of ALDHs in HCC, we extracted The Cancer Genome Atlas (TCGA) data and identified altered patterns of the ALDH genes. In the upregulated groups, we found a 1.16-fold upregulation of the ALDH1A1 gene in primary human HCC compared to normal healthy livers (Table 1). This observation is consistent with previous reports about the identification of ALDH1 in metabolic and gene expression profiles that confer cytotoxicity in HepG2 liver cancer cells.46 ALDH1 activity also has been identified in rabbit hepatic VX2 tumors.47 In addition, ALDH1A1 protein has been found to stabilize the transcription factor GLI family zinc finger 2 (Gli2) and enhance the Hedgehog signaling in HCC.48 ALDH1A1 can also crosstalk with insulin growth factor binding protein 1 in liver metastasis from colorectal cancer.49 Overexpression of the ALDH1A1 gene has been observed to be in differentiated cells but not in cancer stem/progenitor cells in HCC.50 High ALDH1A1 expression is associated with a 57-month recurrence-free survival in hepatitis B virus-related HCC patients.5 Moreover, ALDH3A1 overexpression has been identified in HCC with the Wnt/β-catenin pathway.40 Consistent with this report, we found a 2.8-fold increase in the ALDH3A1 expression in HCC after analyzing the TCGA database (Table 1). ALDH18A1 is another member of metabolic pathways regulating HCC.51 The bifunctional ALDH18A1 gene controls the conversion of glutamate to glutamate 5-semialdehyde in the biosynthesis of proline, ornithine, and arginine. This metabolic axis can support HCC cell survival by modulating hypoxia-inducible factor 1-alpha stability in response to hypoxia.52ALDH18A1 also has been identified as a metabolism-related gene in cholesterol-associated nonalcoholic steatohepatitis-HCCs in mice and humans.53 Furthermore, ALDH18A1 upregulation in liver cancer of both human and animal models is associated with the reprogramming of mitochondrial proline metabolism with pyrroline-5-carboxylate reductase as a potential mechanism of action for the proline pathway in cancer development.54 Reducing H3K18Ac and H3K27Ac levels at the promoter regions of amino acid metabolism and nucleotide synthesis enzyme genes including ALDH18A1 have been found in Huh7 liver cancer cells.55 In agreement with these reports, we found a 1.32-fold increase in the ALDH18A1 gene in 372 primary HCCs from the TCGA database (Table 1). Patients with high levels of this gene exhibited decreased survival rates than those exhibiting lower levels (Table 1). These observations support the protumorigenic role of the ALDH18A1 gene in HCC development. Although ALDH1L2, ALDH3A2, ALDH3B1, ALDH3B2, and ALDH16A1 were upregulated in our analyses of the TCGA database (Table 1), there is no report in the literature about the involvement of these genes in HCC. Nevertheless, we found that ALDH3B1 and ALDH3B2 were not only upregulated but also associated with a poorer prognosis in HCC patients with high levels of these two genes (Table 1). These observations indicated undiscovered protumorigenic activities of ALDH3B1 and ALDH3B2 in the liver.

Table 1

Summary of ALDH expression in human primary hepatocellular carcinoma

ALDH typeMedian value Normal (n = 50)Median value Primary HCC (n = 376)Statistical significance (Normal versus Primary HCC)Statistical significance of ALDH expression with poor prognosis and survival rates
ALDH1A1464.669539.403Up, p = 6.103E−9ap = 0.54
ALDH1A20.5020.407Down, p = 2.623E−6ap = 0.12
ALDH1A31.4360.488p = 0.595p = 0.26
ALDH1B178.7739.793Down, p = 1.639E−5ap = 0.039a
ALDH1L1227.328113.672p = 0.943p = 0.28
ALDH1L20.0430.081Up, p = 7.737E−11ap = 0.83
ALDH2945.96394.334Down, p < 1E−12ap = 0.081
ALDH3A10.4611.297Up, p = 1.923E−6ap = 0.98
ALDH3A287.37287.728Up, p = 4.111E−6ap = 0.93
ALDH3B12.613.837Up, p = 4.921E−13ap = 0.038a
ALDH3B20.0040.015Up, p = 0.030ap = 0.029a
ALDH4A1198.159118.372Down, p = 7.924E−9ap = 0.74
ALDH5A134.6825.422Down, p = 0.0254ap = 0.049a
ALDH6A1103.47229.32Down, p < 1E−12ap = 0.24
ALDH7A164.94156.968p = 0.0636p = 0.041a
ALDH8A1107.05934.109Down, p = 1.625E−12ap = 0.029a
ALDH9A186.3767.501p = 9.198E−4p = 0.94
ALDH16A18.00114.669Up, p < 1E−12 ap = 0.59
ALDH18A114.49719.143Up, p = 1.624E−12ap = 0.0014a

aALDHs with statistical significance (p < 0.05). Data are from The Cancer Genome Atlas database (http://ualcan.path.uab.edu/analysis.html ). ALDH, acetaldehyde dehydrogenase; HCC, hepatocellular carcinoma.

On the other hand, we identified downregulated groups in primary human HCC after analyzing the TCGA database. These downregulated genes included ALDH1A2, ALDH1B1, ALDH2, ALDH4A1, ALDH5A1, ALDH6A1, and ALDH8A1. Consistent with these observations, ALDH1A2 has been found to be downregulated in a pathway-guided computational framework to establish a metabolic signature with the capacity for HCC prognosis prediction.56 We observed 1.233-fold downregulation of ALDH1A2 in primary human HCC compared to normal healthy livers after analyzing extracted data from the TCGA database (Table 1). ALDH1B1 with high expression has displayed protective roles for HCCs with multiple nodules and high serum alpha-fetoprotein levels.5 The protective role of Aldh1b1 also has been shown to inhibit ethanol-induced hepatocellular hyperproliferation and tumor development in rodents.57 Consistent with these previous publications, we found a 1.98-fold downregulation of ALDH1B1 in primary human HCC (Table 1).

ALDH2 is a potential therapeutic target for liver disease.58 This enzyme can alleviate alcoholic liver disease by preventing acetaldehyde exposure in the reduction of signal transducer and activator of transcription 1 methylation.59 It also inhibits oxidative stress and mitochondrial dysfunction in nonalcoholic fatty liver disease.60 Moreover, ALDH2 activity can be antifibrotic and reduce collagen production by regulating NF-E2-related factor 2/antioxidant responsive element and NF-E2-related factor 2/heme oxygenase-1 signaling pathways.61,62 However, the ALDH2 gene is downregulated in many liver diseases. Decreased levels of ALDH2 have been shown to indicate a poor prognosis in HCC patients.63ALDH2 deficiency also has been linked with a higher risk for the progression of alcohol-associated fibrosis to HCC.64 Additionally, ALDH2 loss in hepatocytes has been shown to release copious amounts of oxidized mitochondrial DNAs through extracellular vehicles. Neighboring HCC cells can then take up the extracellular vehicles, containing acetaldehyde, and activate multiple oncogenic pathways that promote carcinogenesis after chronic exposure to alcohol and carbon tetrachloride.64 Another study suggests a negative correlation between the susceptibility to HCC and ALDH2 expression in an HCC-independent cohort.65 A dose-dependent link exists between alcohol consumption over time and the risk of HCC individuals with the ALDH2*1/*2 or ALDH2*2/*2 genotype.66 Potential mechanisms by which ALDH2 contributes to HCC advancement arise from the accumulation of acetaldehyde, which causes the increased activation of the AMP-activated protein kinase pathway. Conversely, metastasis is also affected by ALDH2, since modulating the AMP-activated protein kinase pathway affects lipid metabolism and regulates tumor growth and survival.67 In agreement with these protective roles of ALDH2 for the liver, we observed a 2.4-fold downregulation of this gene in primary HCC (Table 1). This downregulation supports the concept that loss of protective ALDH2 contributes to HCC development.

ALDH5A1 has been identified as one of eight genes in a prognostic HCC model.68 We detected a 1.36-fold decrease in this gene in primary human HCC (Table 1). Both the ALDH2 and ALDH5A1 enzymes can oxidize 4-hydroxy-2-nonenal. The loss of ALDH5A1 implies that, like ALDH2, ALDH5A1 has a protective role in the liver, and its loss may contribute to liver damage during HCC development.

ALDH4A1 has been identified as glutamic gamma-semialdehyde dehydrogenase, and ALDH6A1 has altered levels in HCC. We detected 1.674-fold and 3.53-fold decreases of ALDH4A1 and ALDH6A1, respectively, in primary human HCC (Table 1), implicating the loss of the protective roles of these two genes in HCC development. In agreement with this potential function, both genes have been demonstrated as potential molecular signatures for HCC through quantitative analysis of the mitochondrial proteome.69

ALDH8A1 is reported as one of eight genes associated with prognosis in a risk score assessment model of HCC patients.70 We detected a 3.14-fold decrease in this gene in primary human HCC (Table 1). ALDH1L1 downregulation also has been reported in HCC tumors, and its decreased expression is associated with the poor prognosis of HCC patients.71 The ALDH1L1 variant rs2276724 and mRNA expression predict postoperative clinical outcomes and are associated with tumor protein p53 expression in hepatitis B virus-related HCC.72 Knockout of Aldh1l1 in mice has been demonstrated to reprogram metabolism, thus accelerating HCC.73 The ALDH1L1 promoter is extensively methylated in HCC.74 Additionally, hepatitis B virus-related HCC patients with high ALDH1L1 gene expression had a better clinical outcome. However, we did not observe any statistical significance in the ALDH1L1 gene between primary HCC and controls, although there was a 2-fold decrease of the ALDH1L1 gene in human HCC from the TCGA database (Table 1). In all of the decreased gene groups from the TCGA database, we found statistical significance of a poor prognosis for HCC patients who expressed decreased levels of ALDH1B1, ALDH5A1, ALDH7A1, and ALDH8A1 (Table 1). These results suggest that the loss of expression of these genes in HCC patients was correlated with worse survival rates. Therefore, these genes can be considered promising diagnostic and prognostic markers as well as potential drug targets.

ALDHs and CCA

CCA is a type of liver cancer arising from the epithelium lining the intrahepatic or extrahepatic biliary ducts.75 Intrahepatic CCA is classified as peripheral tumors formed in the bile ducts inside the liver, and it accounts for less than 10% of annual CCA cases.76,77 Hilar or perihilar CCA occurs in the bile ducts just outside of the liver. Distal CCA is also extrahepatic and can arise in the portion of the bile duct nearest the small intestine. Despite different locations, ALDHs have been considered to be molecular markers of CCA stem cells.78,79 To determine whether there are any alterations of ALDHs in CCA, we compared the levels of these genes in CCA tumors after analyzing the TCGA database from 36 CCA cases. As shown in Table 2, ALDH1A1, ALDH2, ALDH1L1, ALDH9A1, ALDH8A1, ALDH5A1, ALDH6A1, ALDH7A1, and ALDH4A1 were downregulated in primary CCA. Similarly, Wang et al. have found downregulation of ALDH1A1, ALDH3A2, ALDH4A1, ALDH6A1, and ALDH18A1; whereas ALDH3B1 and ALDH3B2 are highly induced in tumor tissues compared with the peritumor tissues.80 Downregulation of the ALDH1A1 and ALDH6A1 genes is common in CCA from both analyses based on the TCGA data and the study by Wang et al.80 The mechanism of ALDH1A1 downregulation is known to involve transcriptional regulation by histone H3K27 acetylation in CCA cells.80 Consistent with these reports, significant downregulation of ALDH1A1 (3.86-fold decrease) was detected in primary human CCA compared with normal livers in the TCGA database (Table 2). Other discrepancies in the two analyses appear likely to be due to different sample sizes and controls, since Wang et al. used eight pairs of CCA samples and adjacent tissues, while TCGA has 36 CCA tumors in comparison to nine normal healthy control livers. Another possibility for the discrepancy may be due to the diversity of extrahepatic and intrahepatic CCAs in the TCGA database and the study by Wang et al.80 Considering that sample sizes are small in the TCGA database, we further searched the publicly available Gene Expression Omnibus dataset (https://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE26566 ) that includes 104 freshly frozen CCA tumor samples and 59 matched noncancerous livers obtained from Australia, Europe, and the United States.81 Significant downregulation of ALDH1A1, ALDH1B1, ALDH1L1, ALDH2, ALDH3A2, ALDH4A1, ALDH5A1, ALDH6A1, ALDH7A1, ALDH8A1, and ALDH9A1 as well as upregulation of ALDH1A3, ALDH3B1, and ALDH16A1 were observed (Fig. 3).81 These observations were consistent with the findings from the TCGA data and the study by Wang et al.80

Table 2

Summary of ALDH expression in human primary cholangiocarcinoma

ALDH typeMedian value Normal (n = 9)Median value Primary CC (n = 36)Statistical significance (Normal versus Primary CCA)Statistical significance for ALDH expression with poor prognosis and survival rates
ALDH1A1417.329108.242Down, p = 4.806E−6ap = 0.37
ALDH1A20.61.004Up, p = 0.0487ap = 0.36
ALDH1A31.2754.565Up, p = 1.828E−4ap = 0.82
ALDH1B151.15430.142p = 0.09586p = 0.2
ALDH1L1263.8656.489Down, p = 2.1904E−04ap = 0.25
ALDH1L20.0780.565Up, p = 8.0222E−08ap = 0.62
ALDH21,027.483122.616Down, p = 6.883E−15ap = 0.28
ALDH3A10.5170.603p = 3.528E−01p = 0.15
ALDH3A290.02568.186p = 2.155E−01p = 0.27
ALDH3B12.72823.116Up, p = 3.751E−06ap = 0.85
ALDH3B20.0110.508Up, p = 1.816E−03ap = 0.012 (high level less survival)a
ALDH4A1186.23936.732Down, p = 7.043E−10ap = 0.35
ALDH5A139.28111.463Down, p = 6.481E−12ap = 0.58
ALDH6A188.9827.107Down, p = 1.438E−12ap = 0.56
ALDH7A163.59332.341Down, p = 4.447E−04ap = 0.84
ALDH8A1108.5932.474Down, p < 1E−12ap = 0.44
ALDH9A184.21345.997Down, p = 9.460E−03ap = 0.47
ALDH16A17.91930.301Up, p = 8.576E−12ap = 0.72
ALDH18A110.82233.843Up, p = 2.610E−10ap = 0.5

aALDHs with statistical significance (p < 0.05). The Cancer Genome Atlas dataset “Cholangiocarcinoma” was explored (http://ualcan.path.uab.edu/analysis.html ). Data regarding the mRNA levels or relationship between patients’ survival rate were obtained to search the genes of interest. ALDH, acetaldehyde dehydrogenase; CCA, cholangiocarcinoma.

Altered mRNA levels of <italic>ALDHs</italic> in human cholangiocarcinoma (CCA).
Fig. 3  Altered mRNA levels of ALDHs in human cholangiocarcinoma (CCA).

Graphed data were extracted from a Gene Expression Omnibus dataset (https://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE26566 ) including 104 freshly frozen CCA tumor samples and 59 matched noncancerous adjacent (adj) livers obtained from Australia, Europe, and the United States.81 The gene ID number in the dataset is labeled following the gene name in each graph. ALDH, acetaldehyde dehydrogenase.

ALDH1A3 in CCA plays a vital role in the malignant behavior of CCA and may serve as a new therapeutic target.82 A positive correlation has been identified between the ALDH1A3 protein expression levels and the cell migration abilities of three CCA cell lines, which has been verified using ALDH1A3-overexpressing and ALDH1A3-knockdown clones.83 In addition, lactic acidosis has been shown to upregulate epidermal growth factor receptor and ALDH1A3 expression, leading to the aggressiveness of CCA cells.84 Given the fact that ALDH1A3 is protumorigenic, it is not surprising that this gene displayed a 3.58-fold upregulation in CCA (Table 2). We also found upregulation of the ALDH16A1 (3.83-fold), ALDH1L2 (7.24-fold), ALDH3B1 (8.47-fold), ALDH3B2 (46-fold), and ALDH18A1 genes (3.13-fold) in CCA tumors (Table 2). ALDH8A1, as one of five hub genes, showed higher DNA methylation levels of the promoter in CCA compared with normal liver tissues and has been considered a potential DNA methylation biomarker and therapeutic target in CCA.85ALDH3B2 belongs to the ALDH3 family of the ALDH superfamily.86 Mammalian ALDH3 genes (ALDH3A1, ALDH3A2, ALDH3B1, and ALDH3B2) encode enzymes of peroxidic and fatty aldehyde metabolism.87ALDH3B2 is found in the endoplasmic reticulum. Although its substrates are unknown, suppression of ALDH3B2 expression can inhibit the proliferation and clonogenic ability of CCA cells by inducing G1-phase arrest.88–90ALDH3B2 promotes the proliferation and invasion of CCA by increasing the expression of integrin beta1 and the phosphorylation levels of downstream c-Jun, ERK 1/2, and p38 MAPK. It has been demonstrated as a prognostic factor of CCA.91 Despite its undetectable levels in normal human livers (Fig. 1), ALDH3B2 overexpression is significantly associated with low survival rates in both HCC and CCA patients in our analyses of the TCGA database (Tables 1 and 2). Therefore, ALDH3B2 is an interesting oncogene worthy of further study.

Conclusions

The 19 detoxification ALDH genes exhibit differential spatial and temporal patterns in the liver. In normal conditions, human hepatocytes express ALDH1A1, ALDH1B1, ALDH2, ALDH1L1, ALDH9A1, ALDH8A1, ALDH5A1, ALDH6A1, ALDH3A2, ALDH7A1, and ALDH4A1. Among them, ALDH3A2, ALDH1A1, ALDH16A1, ALDH5A1, ALDH4A1, and ALDH2 are predominately localized in the pericentral zones. In contrast, ALDH1B1, ALDH1A7, ALDH9A1, and ALDH1L1 are mainly expressed in the periportal zones. ALDH7A1 has two peaks in layer 2 and the periportal zone, respectively. ALDH6A1 has peak levels at both the periportal and pericentral areas. ALDH8A1 and ALDH1L2 have the lowest expression in both the periportal and pericentral zones, but ALDH8A1 has a peak in the middle zones, and ALDH1L2 has two peaks in the middle zones. Upregulation of ALDH16A1, ALDH1A1, ALDH1B1, ALDH1L2, ALDH3A1, ALDH3A2, ALDH3B1, ALDH3B2, and ALDH18A1 occur in HCC; whereas ALDH1A2, ALDH2, ALDH8A1, ALDH5A1, ALDH6A1, and ALDH4A1 are downregulated in HCC. Loss of ALDH8A1 and ALDH5A1 as well as upregulation of ALDH1B1, ALDH3B1, ALDH3B2, and ALDH18A1 are associated with a poor prognosis and low survival rates in HCC patients. Moreover, the upregulation of ALDH3B2 is associated with a poor prognosis and low survival rates in CCA patients. These altered expression patterns demonstrate the deregulation of ALDHs in the development of HCC and CCA. Whether there are additional changes of the deregulated ALDHs during liver injury and cancer development warrants further investigation. Further understanding of ALDH genes in the liver, in particular their relation to liver zonation, may help us to develop more accurate and personalized strategies for the treatment of liver diseases such as HCC and CCA.

Abbreviations

ALDH: 

acetaldehyde dehydrogenase

AMP: 

adenosine monophosphate

Arg1: 

arginase 1

CCA: 

cholangiocarcinoma

EC: 

vascular endothelial cells

HCC: 

hepatocellular carcinoma

HSCs: 

hepatic stellate cells

TCGA: 

The Cancer Genome Atlas

Declarations

Acknowledgement

None.

Funding

This study was supported by a National Institute on Alcohol Abuse and Alcoholism grant (RO1AA028035) awarded to LP.

Conflict of interest

The authors have no conflict of interests related to this publication.

Authors’ contributions

All authors wrote this review paper and generated the figures and table.

References

  1. Vasiliou V, Nebert DW. Analysis and update of the human aldehyde dehydrogenase (ALDH) gene family. Hum Genomics 2005;2(2):138-143 View Article PubMed/NCBI
  2. Molotkov A, Duester G. Genetic evidence that retinaldehyde dehydrogenase Raldh1 (Aldh1a1) functions downstream of alcohol dehydrogenase Adh1 in metabolism of retinol to retinoic acid. J Biol Chem 2003;278(38):36085-36090 View Article PubMed/NCBI
  3. Kim JI, Ganesan S, Luo SX, Wu YW, Park E, Huang EJ, et al. Aldehyde dehydrogenase 1a1 mediates a GABA synthesis pathway in midbrain dopaminergic neurons. Science 2015;350(6256):102-106 View Article PubMed/NCBI
  4. Kiefer FW, Orasanu G, Nallamshetty S, Brown JD, Wang H, Luger P, et al. Retinaldehyde dehydrogenase 1 coordinates hepatic gluconeogenesis and lipid metabolism. Endocrinology 2012;153(7):3089-3099 View Article PubMed/NCBI
  5. Yang CK, Wang XK, Liao XW, Han CY, Yu TD, Qin W, et al. Aldehyde dehydrogenase 1 (ALDH1) isoform expression and potential clinical implications in hepatocellular carcinoma. PLoS One 2017;12(8):e0182208 View Article PubMed/NCBI
  6. Peng GS, Yin SJ. Effect of the allelic variants of aldehyde dehydrogenase ALDH2*2 and alcohol dehydrogenase ADH1B*2 on blood acetaldehyde concentrations. Hum Genomics 2009;3(2):121-127 View Article PubMed/NCBI
  7. Deitrich RA, Petersen D, Vasiliou V. Removal of acetaldehyde from the body. Novartis Found Symp 2007;285:23-40 View Article PubMed/NCBI
  8. Perozich J, Nicholas H, Wang BC, Lindahl R, Hempel J. Relationships within the aldehyde dehydrogenase extended family. Protein Sci 1999;8(1):137-146 View Article PubMed/NCBI
  9. Sass JO, Walter M, Shield JP, Atherton AM, Garg U, Scott D, et al. 3-Hydroxyisobutyrate aciduria and mutations in the ALDH6A1 gene coding for methylmalonate semialdehyde dehydrogenase. J Inherit Metab Dis 2012;35(3):437-442 View Article PubMed/NCBI
  10. Forte-McRobbie CM, Pietruszko R. Purification and characterization of human liver “high Km” aldehyde dehydrogenase and its identification as glutamic gamma-semialdehyde dehydrogenase. J Biol Chem 1986;261(5):2154-2163 PubMed/NCBI
  11. Brocker C, Cantore M, Failli P, Vasiliou V. Aldehyde dehydrogenase 7A1 (ALDH7A1) attenuates reactive aldehyde and oxidative stress induced cytotoxicity. Chem Biol Interact 2011;191(1-3):269-277 View Article PubMed/NCBI
  12. Davis I, Yang Y, Wherritt D, Liu A. Reassignment of the human aldehyde dehydrogenase ALDH8A1 (ALDH12) to the kynurenine pathway in tryptophan catabolism. J Biol Chem 2018;293(25):9594-9603 View Article PubMed/NCBI
  13. Islam MS, Ghosh A. Evolution, family expansion, and functional diversification of plant aldehyde dehydrogenases. Gene 2022;829:146522 View Article PubMed/NCBI
  14. Pavan M, Ruiz VF, Silva FA, Sobreira TJ, Cravo RM, Vasconcelos M, et al. ALDH1A2 (RALDH2) genetic variation in human congenital heart disease. BMC Med Genet 2009;10:113 View Article PubMed/NCBI
  15. Lin S, Harlalka GV, Hameed A, Reham HM, Yasin M, Muhammad N, et al. Novel mutations in ALDH1A3 associated with autosomal recessive anophthalmia/microphthalmia, and review of the literature. BMC Med Genet 2018;19(1):160 View Article PubMed/NCBI
  16. Husemoen LL, Fenger M, Friedrich N, Tolstrup JS, Beenfeldt Fredriksen S, Linneberg A. The association of ADH and ALDH gene variants with alcohol drinking habits and cardiovascular disease risk factors. Alcohol Clin Exp Res 2008;32(11):1984-1991 View Article PubMed/NCBI
  17. Linneberg A, Gonzalez-Quintela A, Vidal C, Jørgensen T, Fenger M, Hansen T, et al. Genetic determinants of both ethanol and acetaldehyde metabolism influence alcohol hypersensitivity and drinking behaviour among Scandinavians. Clin Exp Allergy 2010;40(1):123-130 View Article PubMed/NCBI
  18. Krupenko SA, Oleinik NV. 10-formyltetrahydrofolate dehydrogenase, one of the major folate enzymes, is down-regulated in tumor tissues and possesses suppressor effects on cancer cells. Cell Growth Differ 2002;13(5):227-236 PubMed/NCBI
  19. Kinjo Y, Cui Y, Akiba S, Watanabe S, Yamaguchi N, Sobue T, et al. Mortality risks of oesophageal cancer associated with hot tea, alcohol, tobacco and diet in Japan. J Epidemiol 1998;8(4):235-243 View Article PubMed/NCBI
  20. Brown LM, Devesa SS. Epidemiologic trends in esophageal and gastric cancer in the United States. Surg Oncol Clin N Am 2002;11(2):235-256 View Article PubMed/NCBI
  21. Zhang Y, Ren J. ALDH2 in alcoholic heart diseases: molecular mechanism and clinical implications. Pharmacol Ther 2011;132(1):86-95 View Article PubMed/NCBI
  22. Bui AL, Horwich TB, Fonarow GC. Epidemiology and risk profile of heart failure. Nat Rev Cardiol 2011;8(1):30-41 View Article PubMed/NCBI
  23. Xu F, Chen Y, Lv R, Zhang H, Tian H, Bian Y, et al. ALDH2 genetic polymorphism and the risk of type II diabetes mellitus in CAD patients. Hypertens Res 2010;33(1):49-55 View Article PubMed/NCBI
  24. Ma H, Guo R, Yu L, Zhang Y, Ren J. Aldehyde dehydrogenase 2 (ALDH2) rescues myocardial ischaemia/reperfusion injury: role of autophagy paradox and toxic aldehyde. Eur Heart J 2011;32(8):1025-1038 View Article PubMed/NCBI
  25. Wang B, Wang J, Zhou S, Tan S, He X, Yang Z, et al. The association of mitochondrial aldehyde dehydrogenase gene (ALDH2) polymorphism with susceptibility to late-onset Alzheimer’s disease in Chinese. J Neurol Sci 2008;268(1-2):172-175 View Article PubMed/NCBI
  26. Yoshida A, Hsu LC, Yasunami M. Genetics of human alcohol-metabolizing enzymes. Prog Nucleic Acid Res Mol Biol 1991;40:255-287 View Article PubMed/NCBI
  27. Yokoyama A, Muramatsu T, Omori T, Yokoyama T, Matsushita S, Higuchi S, et al. Alcohol and aldehyde dehydrogenase gene polymorphisms and oropharyngolaryngeal, esophageal and stomach cancers in Japanese alcoholics. Carcinogenesis 2001;22(3):433-439 View Article PubMed/NCBI
  28. Rizzo WB. Sjögren-Larsson syndrome: molecular genetics and biochemical pathogenesis of fatty aldehyde dehydrogenase deficiency. Mol Genet Metab 2007;90(1):1-9 View Article PubMed/NCBI
  29. Motte J, Fisse AL, Grüter T, Schneider R, Breuer T, Lücke T, et al. Novel variants in a patient with late-onset hyperprolinemia type II: Diagnostic key for status epilepticus and lactic acidosis. BMC Neurol 2019;19(1):345 View Article PubMed/NCBI
  30. Akaboshi S, Hogema BM, Novelletto A, Malaspina P, Salomons GS, Maropoulos GD, et al. Mutational spectrum of the succinate semialdehyde dehydrogenase (ALDH5A1) gene and functional analysis of 27 novel disease-causing mutations in patients with SSADH deficiency. Hum Mutat 2003;22(6):442-450 View Article PubMed/NCBI
  31. Tlili A, Hamida Hentati N, Gargouri A, Fakhfakh F. Identification of a novel missense mutation in the ALDH7A1 gene in two unrelated Tunisian families with pyridoxine-dependent epilepsy. Mol Biol Rep 2013;40(1):487-490 View Article PubMed/NCBI
  32. Vasiliou V, Sandoval M, Backos DS, Jackson BC, Chen Y, Reigan P, et al. ALDH16A1 is a novel non-catalytic enzyme that may be involved in the etiology of gout via protein-protein interactions with HPRT1. Chem Biol Interact 2013;202(1-3):22-31 View Article PubMed/NCBI
  33. Bicknell LS, Pitt J, Aftimos S, Ramadas R, Maw MA, Robertson SP. A missense mutation in ALDH18A1, encoding Delta1-pyrroline-5-carboxylate synthase (P5CS), causes an autosomal recessive neurocutaneous syndrome. Eur J Hum Genet 2008;16(10):1176-1186 View Article PubMed/NCBI
  34. Fagerberg L, Hallström BM, Oksvold P, Kampf C, Djureinovic D, Odeberg J, et al. Analysis of the human tissue-specific expression by genome-wide integration of transcriptomics and antibody-based proteomics. Mol Cell Proteomics 2014;13(2):397-406 View Article PubMed/NCBI
  35. Hoehme S, Brulport M, Bauer A, Bedawy E, Schormann W, Hermes M, et al. Prediction and validation of cell alignment along microvessels as order principle to restore tissue architecture in liver regeneration. Proc Natl Acad Sci USA 2010;107(23):10371-10376 View Article PubMed/NCBI
  36. Halpern KB, Shenhav R, Matcovitch-Natan O, Toth B, Lemze D, Golan M, et al. Single-cell spatial reconstruction reveals global division of labour in the mammalian liver. Nature 2017;542(7641):352-356 View Article PubMed/NCBI
  37. Bahar Halpern K, Tanami S, Landen S, Chapal M, Szlak L, Hutzler A, et al. Bursty gene expression in the intact mammalian liver. Mol Cell 2015;58(1):147-156 View Article PubMed/NCBI
  38. Ben-Moshe S, Shapira Y, Moor AE, Manco R, Veg T, Bahar Halpern K, et al. Spatial sorting enables comprehensive characterization of liver zonation. Nat Metab 2019;1(9):899-911 View Article PubMed/NCBI
  39. Torre C, Perret C, Colnot S. Transcription dynamics in a physiological process: β-catenin signaling directs liver metabolic zonation. Int J Biochem Cell Biol 2011;43(2):271-278 View Article PubMed/NCBI
  40. Calderaro J, Nault JC, Bioulac-Sage P, Laurent A, Blanc JF, Decaens T, et al. ALDH3A1 is overexpressed in a subset of hepatocellular carcinoma characterised by activation of the Wnt/ß-catenin pathway. Virchows Arch 2014;464(1):53-60 View Article PubMed/NCBI
  41. Condello S, Morgan CA, Nagdas S, Cao L, Turek J, Hurley TD, et al. β-Catenin-regulated ALDH1A1 is a target in ovarian cancer spheroids. Oncogene 2015;34(18):2297-2308 View Article PubMed/NCBI
  42. Zhou J, Sun C, Yang L, Wang J, Jn-Simon N, Zhou C, et al. Liver regeneration and ethanol detoxification: A new link in YAP regulation of ALDH1A1 during alcohol-related hepatocyte damage. FASEB J 2022;36(4):e22224 View Article PubMed/NCBI
  43. Bertola A, Mathews S, Ki SH, Wang H, Gao B. Mouse model of chronic and binge ethanol feeding (the NIAAA model). Nat Protoc 2013;8(3):627-637 View Article PubMed/NCBI
  44. Llovet JM, Zucman-Rossi J, Pikarsky E, Sangro B, Schwartz M, Sherman M, et al. Hepatocellular carcinoma. Nat Rev Dis Primers 2016;2:16018 View Article PubMed/NCBI
  45. Villanueva A. Hepatocellular Carcinoma. N Engl J Med 2019;380(15):1450-1462 View Article PubMed/NCBI
  46. Li Z, Srivastava S, Yang X, Mittal S, Norton P, Resau J, et al. A hierarchical approach employing metabolic and gene expression profiles to identify the pathways that confer cytotoxicity in HepG2 cells. BMC Syst Biol 2007;1:21 View Article PubMed/NCBI
  47. Gehlot P, Shukla V, Gupta S, Makidon PE. Detection of ALDH1 activity in rabbit hepatic VX2 tumors and isolation of ALDH1 positive cancer stem cells. J Transl Med 2016;14:49 View Article PubMed/NCBI
  48. Yan Z, Xu L, Zhang J, Lu Q, Luo S, Xu L. Aldehyde dehydrogenase 1A1 stabilizes transcription factor Gli2 and enhances the activity of Hedgehog signaling in hepatocellular cancer. Biochem Biophys Res Commun 2016;471(4):466-473 View Article PubMed/NCBI
  49. Kim JC, Ha YJ, Tak KH, Roh SA, Kim CW, Kim TW, et al. Complex Behavior of ALDH1A1 and IGFBP1 in Liver Metastasis from a Colorectal Cancer. PLoS One 2016;11(5):e0155160 View Article PubMed/NCBI
  50. Tanaka K, Tomita H, Hisamatsu K, Nakashima T, Hatano Y, Sasaki Y, et al. ALDH1A1-overexpressing cells are differentiated cells but not cancer stem or progenitor cells in human hepatocellular carcinoma. Oncotarget 2015;6(28):24722-24732 View Article PubMed/NCBI
  51. Ding Z, Ericksen RE, Escande-Beillard N, Lee QY, Loh A, Denil S, et al. Metabolic pathway analyses identify proline biosynthesis pathway as a promoter of liver tumorigenesis. J Hepatol 2020;72(4):725-735 View Article PubMed/NCBI
  52. Tang L, Zeng J, Geng P, Fang C, Wang Y, Sun M, et al. Global metabolic profiling identifies a pivotal role of proline and hydroxyproline metabolism in supporting hypoxic response in hepatocellular carcinoma. Clin Cancer Res 2018;24(2):474-485 View Article PubMed/NCBI
  53. Liang JQ, Teoh N, Xu L, Pok S, Li X, Chu ESH, et al. Dietary cholesterol promotes steatohepatitis related hepatocellular carcinoma through dysregulated metabolism and calcium signaling. Nat Commun 2018;9(1):4490 View Article PubMed/NCBI
  54. Ding Z, Ericksen RE, Lee QY, Han W. Reprogramming of mitochondrial proline metabolism promotes liver tumorigenesis. Amino Acids 2021;53(12):1807-1815 View Article PubMed/NCBI
  55. Cai LY, Chen SJ, Xiao SH, Sun QJ, Ding CH, Zheng BN, et al. Targeting p300/CBP attenuates hepatocellular carcinoma progression through epigenetic regulation of metabolism. Cancer Res 2021;81(4):860-872 View Article PubMed/NCBI
  56. Shi Q, Liu Y, Lu M, Lei QY, Chen Z, Wang L, et al. A pathway-guided strategy identifies a metabolic signature for prognosis prediction and precision therapy for hepatocellular carcinoma. Comput Biol Med 2022;144:105376 View Article PubMed/NCBI
  57. Müller MF, Kendall TJ, Adams DJ, Zhou Y, Arends MJ. The murine hepatic sequelae of long-term ethanol consumption are sex-specific and exacerbated by Aldh1b1 loss. Exp Mol Pathol 2018;105(1):63-70 View Article PubMed/NCBI
  58. Wu YC, Yao Y, Tao LS, Wang SX, Hu Y, Li LY, et al. The role of acetaldehyde dehydrogenase 2 in the pathogenesis of liver diseases. Cell Signal 2023;102:110550 View Article PubMed/NCBI
  59. Ganesan M, Poluektova LY, Enweluzo C, Kharbanda KK, Osna NA. Hepatitis C Virus-Infected Apoptotic Hepatocytes Program Macrophages and Hepatic Stellate Cells for Liver Inflammation and Fibrosis Development: Role of Ethanol as a Second Hit. Biomolecules 2018;8(4):113 View Article PubMed/NCBI
  60. Yang SS, Chen YH, Hu JT, Chiu CF, Hung SW, Chang YC, et al. Aldehyde dehydrogenase mutation exacerbated high-fat-diet-induced nonalcoholic fatty liver disease with gut microbiota remodeling in male mice. Biology (Basel) 2021;10(8):737 View Article PubMed/NCBI
  61. Bardallo RG, da Silva RT, Carbonell T, Folch-Puy E, Palmeira C, Roselló-Catafau J, et al. Role of PEG35, mitochondrial ALDH2, and glutathione in cold fatty liver graft preservation: An IGL-2 approach. Int J Mol Sci 2021;22(10):5332 View Article PubMed/NCBI
  62. Ma X, Luo Q, Zhu H, Liu X, Dong Z, Zhang K, et al. Aldehyde dehydrogenase 2 activation ameliorates CCl4 -induced chronic liver fibrosis in mice by up-regulating Nrf2/HO-1 antioxidant pathway. J Cell Mol Med 2018;22(8):3965-3978 View Article PubMed/NCBI
  63. Jin S, Chen J, Chen L, Histen G, Lin Z, Gross S, et al. ALDH2(E487K) mutation increases protein turnover and promotes murine hepatocarcinogenesis. Proc Natl Acad Sci USA 2015;112(29):9088-9093 View Article PubMed/NCBI
  64. Seo W, Gao Y, He Y, Sun J, Xu H, Feng D, et al. ALDH2 deficiency promotes alcohol-associated liver cancer by activating oncogenic pathways via oxidized DNA-enriched extracellular vesicles. J Hepatol 2019;71(5):1000-1011 View Article PubMed/NCBI
  65. Hou G, Chen L, Liu G, Li L, Yang Y, Yan HX, et al. Aldehyde dehydrogenase-2 (ALDH2) opposes hepatocellular carcinoma progression by regulating AMP-activated protein kinase signaling in mice. Hepatology 2017;65(5):1628-1644 View Article PubMed/NCBI
  66. Ding J, Li S, Wu J, Gao C, Zhou J, Cao H, et al. Alcohol dehydrogenase-2 and aldehyde dehydrogenase-2 genotypes, alcohol drinking and the risk of primary hepatocellular carcinoma in a Chinese population. Asian Pac J Cancer Prev 2008;9(1):31-35 PubMed/NCBI
  67. Wang W, Wang C, Xu H, Gao Y. Aldehyde dehydrogenase, liver disease and cancer. Int J Biol Sci 2020;16(6):921-934 View Article PubMed/NCBI
  68. Guo DZ, Huang A, Wang YP, Cao Y, Fan J, Yang XR, et al. Development of an Eight-gene Prognostic Model for Overall Survival Prediction in Patients with Hepatocellular Carcinoma. J Clin Transl Hepatol 2021;9(6):898-908 View Article PubMed/NCBI
  69. Shin H, Cha HJ, Lee MJ, Na K, Park D, Kim CY, et al. Identification of ALDH6A1 as a potential molecular signature in hepatocellular carcinoma via quantitative profiling of the mitochondrial proteome. J Proteome Res 2020;19(4):1684-1695 View Article PubMed/NCBI
  70. He J, Zhao H, Deng D, Wang Y, Zhang X, Zhao H, et al. Screening of significant biomarkers related with prognosis of liver cancer by lncRNA-associated ceRNAs analysis. J Cell Physiol 2020;235(3):2464-2477 View Article PubMed/NCBI
  71. Chen XQ, He JR, Wang HY. Decreased expression of ALDH1L1 is associated with a poor prognosis in hepatocellular carcinoma. Med Oncol 2012;29(3):1843-1849 View Article PubMed/NCBI
  72. Zhu G, Liao X, Han C, Liu X, Yu L, Qin W, et al. ALDH1L1 variant rs2276724 and mRNA expression predict post-operative clinical outcomes and are associated with TP53 expression in HBV-related hepatocellular carcinoma. Oncol Rep 2017;38(3):1451-1463 View Article PubMed/NCBI
  73. Krupenko NI, Sharma J, Fogle HM, Pediaditakis P, Strickland KC, Du X, et al. Knockout of putative tumor suppressor aldh1l1 in mice reprograms metabolism to accelerate growth of Tumors in a Diethylnitrosamine (DEN) model of liver carcinogenesis. Cancers (Basel) 2021;13(13):3219 View Article PubMed/NCBI
  74. Oleinik NV, Krupenko NI, Krupenko SA. Epigenetic Silencing of ALDH1L1, a Metabolic Regulator of Cellular Proliferation, in Cancers. Genes Cancer 2011;2(2):130-139 View Article PubMed/NCBI
  75. Banales JM, Marin JJG, Lamarca A, Rodrigues PM, Khan SA, Roberts LR, et al. Cholangiocarcinoma 2020: the next horizon in mechanisms and management. Nat Rev Gastroenterol Hepatol 2020;17(9):557-588 View Article PubMed/NCBI
  76. DeOliveira ML, Cunningham SC, Cameron JL, Kamangar F, Winter JM, Lillemoe KD, et al. Cholangiocarcinoma: thirty-one-year experience with 564 patients at a single institution. Ann Surg 2007;245(5):755-762 View Article PubMed/NCBI
  77. Deoliveira ML, Schulick RD, Nimura Y, Rosen C, Gores G, Neuhaus P, et al. New staging system and a registry for perihilar cholangiocarcinoma. Hepatology 2011;53(4):1363-1371 View Article PubMed/NCBI
  78. Wang M, Xiao J, Jiang J, Qin R. CD133 and ALDH may be the molecular markers of cholangiocarcinoma stem cells. Int J Cancer 2011;128(8):1996-1997 View Article PubMed/NCBI
  79. Shuang ZY, Wu WC, Xu J, Lin G, Liu YC, Lao XM, et al. Transforming growth factor-β1-induced epithelial-mesenchymal transition generates ALDH-positive cells with stem cell properties in cholangiocarcinoma. Cancer Lett 2014;354(2):320-328 View Article PubMed/NCBI
  80. Yoshino J, Akiyama Y, Shimada S, Ogura T, Ogawa K, Ono H, et al. Loss of ARID1A induces a stemness gene ALDH1A1 expression with histone acetylation in the malignant subtype of cholangiocarcinoma. Carcinogenesis 2020;41(6):734-742 View Article PubMed/NCBI
  81. Andersen JB, Spee B, Blechacz BR, Avital I, Komuta M, Barbour A, et al. Genomic and genetic characterization of cholangiocarcinoma identifies therapeutic targets for tyrosine kinase inhibitors. Gastroenterology 2012;142(4):1021-1031.e15 View Article PubMed/NCBI
  82. Chen MH, Weng JJ, Cheng CT, Wu RC, Huang SC, Wu CE, et al. ALDH1A3, the major aldehyde dehydrogenase isoform in human cholangiocarcinoma cells, affects prognosis and gemcitabine resistance in cholangiocarcinoma patients. Clin Cancer Res 2016;22(16):4225-4235 View Article PubMed/NCBI
  83. Chung SY, Hung YP, Pan YR, Chang YC, Wu CE, Hsu DS, et al. Ruxolitinib combined with gemcitabine against cholangiocarcinoma growth via the JAK2/STAT1/3/ALDH1A3 pathway. Biomedicines 2021;9(8):885 View Article PubMed/NCBI
  84. Thamrongwaranggoon U, Detarya M, Seubwai W, Saengboonmee C, Hino S, Koga T, et al. Lactic acidosis promotes aggressive features of cholangiocarcinoma cells via upregulating ALDH1A3 expression through EGFR axis. Life Sci 2022;302:120648 View Article PubMed/NCBI
  85. Chen D, Wu H, He B, Lu Y, Wu W, Liu H, et al. Five hub genes can be the potential DNA methylation biomarkers for cholangiocarcinoma using bioinformatics analysis. Onco Targets Ther 2019;12:8355-8365 View Article PubMed/NCBI
  86. Holmes RS. Biochemical genetics of opossum aldehyde dehydrogenase 3: Evidence for three ALDH3A-like genes and an ALDH3B-like gene. Biochem Genet 2010;48(3-4):287-303 View Article PubMed/NCBI
  87. Holmes RS, Hempel J. Comparative studies of vertebrate aldehyde dehydrogenase 3: sequences, structures, phylogeny and evolution. Evidence for a mammalian origin for the ALDH3A1 gene. Chem Biol Interact 2011;191(1-3):113-121 View Article PubMed/NCBI
  88. Puttini S, Plaisance I, Barile L, Cervio E, Milano G, Marcato P, et al. ALDH1A3 is the key isoform that contributes to aldehyde dehydrogenase activity and affects in Vitro proliferation in cardiac atrial appendage progenitor cells. Front Cardiovasc Med 2018;5:90 View Article PubMed/NCBI
  89. Xie X, Urabe G, Marcho L, Stratton M, Guo LW, Kent CK. ALDH1A3 Regulations of Matricellular Proteins Promote Vascular Smooth Muscle Cell Proliferation. iScience 2019;19:872-882 View Article PubMed/NCBI
  90. Muzio G, Maggiora M, Paiuzzi E, Oraldi M, Canuto RA. Aldehyde dehydrogenases and cell proliferation. Free Radic Biol Med 2012;52(4):735-746 View Article PubMed/NCBI
  91. Wang Y, Li K, Zhao W, Liu Z, Liu J, Shi A, et al. Aldehyde dehydrogenase 3B2 promotes the proliferation and invasion of cholangiocarcinoma by increasing Integrin Beta 1 expression. Cell Death Dis 2021;12(12):1158 View Article PubMed/NCBI
Copyright © 2023 Authors. This is an Open Access article distributed under the terms of the Creative Commons Attribution-Noncommercial 4.0 License (CC BY-NC 4.0), permitting all non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.
  • Gene Expression
  • eISSN 1555-3884

Article Options

Metrics

Cite this Article

  • © 2024 Xia & He Publishing Inc.
  • Total visits : 2641769
  • Visits today : 583
Back to Top

Acetaldehyde Dehydrogenases in Liver Zonation and Liver Cancer

Brady Jin-Smith, Natacha Jn-Simon, Sreenivasulu Basha, Chunbao Sun, Shang Wu, Joshua Max Barkin, Liya Pi
  • Reset Zoom
  • Download TIFF