v
Search
Advanced Search

Publications > Journals > Journal of Clinical and Translational Hepatology > Article Full Text

  • Review Article
  • OPEN ACCESS

Portal Hypertension in Nonalcoholic Fatty Liver Disease: From Pathogenesis to Clinical Practice

  • Saut Horas H. Nababan1,2 and
  • Cosmas Rinaldi Adithya Lesmana1,2,3,* 
 Author information  Cite
Journal of Clinical and Translational Hepatology   2022;10(5):979-985

doi: 10.14218/JCTH.2021.00593

Abstract

Portal hypertension in nonalcoholic fatty liver disease (NAFLD) mostly occur in cirrhotic stage. However, several experimental and clinical studies showed evidence of portal hypertension in NAFLD without significant or advance fibrosis. This early development of portal hypertension in NAFLD is associated with liver sinusoidal contraction by hepatocellular lipid accumulation and ballooning, which is also accompanied by capillarization and dysfunction of liver sinusoidal endothelial cells. Both of these impaired mechanical and molecular components can cause an increase in intrahepatic vascular resistance which lead to the increase of portal pressure in the absence of significant liver fibrosis. Extrahepatic factors such as insulin resistance and gut dysbiosis may also contribute to liver sinusoidal endothelial dysfunction and early portal hypertension in NAFLD. The clinical impact of early portal hypertension in NAFLD is still unclear. However, clinical tools for diagnosis and monitoring of portal hypertension in NAFLD are being investigated to predict high-risk patients and to guide therapy.

Graphical Abstract

Keywords

Portal hypertension, NAFLD, NASH, Metabolic

Introduction

NAFLD is a growing problem in western countries as well as in Asian countries. The prevalence of NAFLD has shown an increasing trend globally. A systemic review and meta-analysis by Younossi et al.1 estimated an overall 25.24% global prevalence of NAFLD. The highest prevalence was in the Middle East (31.79%) and South America (30.45%). Another systematic review and meta-analysis by Li et al.2 focusing on Asian studies from 1999 to 2019, showed an increasing trend of NAFLD cases, from 25% in 1995–2005 to 34% in 2012–2017. A higher prevalence of around 51% was reported by a study in an urban population in Indonesia.3 As NAFLD has a close association with multiple metabolic comorbidities, an updated definition using new terminology of metabolic dysfunction-associated fatty liver disease or MAFLD has been suggested. Using MAFLD criteria, a systemic review and meta-analysis by Liu et al.4 showed that among overweight or obese patients, the estimated global prevalence of MAFLD was 50.7%.

Similar to viral or alcohol etiologies, NAFLD can progress to liver cirrhosis. Most patients are asymptomatic until complications of portal hypertension develop. A subset of NAFLD patients can progress to nonalcoholic steatohepatitis (NASH), which is a risk factor of cirrhosis progression and hepatocellular carcinoma (HCC) development.5 According to survey data of the USA’s National Health and Nutrition Examination Survey, there were 2.5-fold and 2-fold increases in the prevalence of NASH cirrhosis and NAFLD-associated advanced fibrosis, respectively, in 2009–2012 compared with 1999–2002.6 Furthermore, in the USA, NASH and alcoholic liver disease are the most common etiologies among liver transplant waiting list registrant without any evidence of HCC.7

The reported prevalence of portal hypertension in compensated advanced liver disease (cALD) because of NASH, defined as a hepatic venous pressure gradient (HVPG) ≥5 mmHg, is 60.9%, while the prevalence of clinically significant portal hypertension (CSPH) defined as an HVPG ≥10 mmHg is 39.1%.8 Mendes et al.9 showed that complications related to CSPH, such as esophageal varices, splenomegaly, portosystemic encephalopathy, and ascites, were present in 25% of NAFLD patients, 88% of whom had already developed cirrhosis or advance fibrosis. Interestingly, it was reported that 12% of NAFLD patient with signs of portal hypertension had no significant fibrosis (F0–F2), but had severe-grade steatosis. In their retrospective analysis, Rodriques et al.10 found that in 14 of 89 patients with CSPH, mostly were NASH or with nodular regenerative hyperplasia without cirrhosis but with perisinusoidal fibrosis and eight of them had hepatocyte ballooning. The presence of mild portal hypertension, an HVPG of 5–9 mmHg, has also been reported in a small number of noncirrhotic NAFLD patients, suggesting that steatosis per se also contributed to increased portal pressure.11–13 The studies suggest that portal hypertension can develop early during the natural history of NAFLD before development of significant fibrosis or cirrhosis. In this review we look into the pathogenesis, diagnosis, and therapy of portal hypertension in NAFLD.

Pathogenesis

General pathogenesis of portal hypertension

Portal hypertension (PH) can be divided into three groups based on the site of resistance, which are presinusoidal, sinusoidal, and post-sinusoidal. Sinusoidal PH is the most common in advanced liver disease of any etiology. The primary change in sinusoidal PH is an increase in intrahepatic vascular resistance (IHVR). The IHVR is mainly caused by distortion of structural components such as fibrosis and regenerative nodules. The structural changes are accompanied by an increase in intrahepatic vascular tone because of endothelial dysfunction secondary to an imbalance of increased vasoconstrictors and decreased vasodilator stimuli. Furthermore, an increase in portal venous inflow because of splanchnic vasodilatation and increase in cardiac output further exacerbates the portal pressure. Increase in the portal pressure leads to formation of portosystemic collateral vessels and varices. The evidence suggest that angiogenesis also contributes to the formation of portosystemic collateral vessels.14

Liver steatosis contributes to the IHVR by sinusoidal compression and capillarization of endothelial cells

The main histopathological features of NAFLD include >5% steatotic hepatocytes with a centrilobular distribution, hepatocyte ballooning, lobular inflammation, and perisinusoidal fibrosis in NASH. With disease progression, fibrous septa, bridging fibrosis, and cirrhosis will eventually develop. Several clinical studies have shown a correlation between steatosis grade and portal pressure in NAFLD patients, and the data suggest that an early increase in portal pressure can occur without any evidence of significant fibrosis.9,11,12 Steatotic changes in NAFLD can contribute to increased portal pressure by sinusoidal compression and reduced vascular compliance, as shown by measurement of portal venous pulsatile flow and flow velocity. Several studies have found a negative correlation between portal venous pulsatility and flow velocity with steatosis and fibrosis grade in NAFLD patients.15–17 Using advanced tools such as intravital microscopy, Davis et al.18 was able to show in real time that the sinusoidal diameter was significantly lower around steatotic hepatocytes in high-fat diet-fed C57BL/6 mice. Using a methionine choline-deficient diet to induce NASH in C57BL/6J mice, McCuskey et al.19 reported a significant narrowing of the sinusoid lumen, especially in the centrilobular region in as early as 3 weeks after feeding began.19 Morphological changes in liver sinusoidal endothelial cells (LSEC) in early NAFLD/NASH have also been reported. In healthy livers, LSECs are fenestrated, porous, and without a basement membrane. Miyao et al.20 reported that as early as 1 week after starting a choline-deficient L-amino acid defined diet (early steatosis model) and 22 weeks after a high-fat diet in a mice (early NASH model), there was a capillarization or defenestration with reduction in porosity of the LSEC followed by Kupffer cell and HSC activation. In human NAFLD, using CD31 as a marker of LSEC capillarization, increased expression of CD31 was detected in the centrilobular area (zone 3).21 Excessive dietary lipid or glucose may trigger LSEC capillarization in NAFLD. An in vitro study showed that when primary human LSECs were treated with ox-LDL, the fenestral diameter and porosity of LSECs were reduced, and the responses were mediated via the LOX1/ROS/NF-kB signaling pathway.22 The defenestration of LSECs may then promote liver steatosis, creating a vicious cycle in NAFLD disease progression. Plasmalemma vesicle-associated protein (PLVAP), is an endothelial-specific integral membrane glycoprotein required for the formation of endothelial fenestrae. Mice deficient in PLVAP showed a reduced number of LSEC fenestration with impaired passage of chylomicron remnant from sinusoidal into hepatocytes. Lack of chylomicron remnants by the hepatocytes might then stimulate de novo lipogenesis or endogenous cholesterol biosynthesis.23 That, in turn, further augments liver steatosis and an increase in the IHVR. Furthermore, restoration of LSEC porosity by returning to a normal diet or statin administration may reduce portal pressure.24

Liver steatosis contributes to IHVR through sinusoidal endothelial dysfunction

Sinusoidal endothelial dysfunction refers to the inability of LSEC to expand in response to the shear stress of blood flow. It is characterized by diminished bioavailability of vasodilators such as nitric oxide (NO) and increased synthesis of vasoconstrictors such as endothelin-1 (ET-1), resulting in increased intrahepatic vascular resistance (IHVR). Early NAFLD is associated with LSEC dysfunction and increased oxidative stress in the absence of inflammation or fibrosis.25 Pasarin et al.26 showed that when Wistar Kyoto rats were fed a diet rich in saturated fat for 1 month, the in vivo portal pressure was increased and the Akt-dependent endothelial nitric oxide synthase (eNOS) phosphorylation and NOS activity were decreased in the absence of liver inflammation and fibrosis. There was also a decreased response to vasodilator acetylcholine in isolated liver perfusion experiments. Similar results were also reported by Francque et al.27 in methionine choline-deficient diet-fed rats. They showed that the liver expression of vasoconstrictor ET-1 was significantly increased. In healthy LSECs, NO keeps Kupffer cells and hepatic stellate cells (HSC) in a quiescent state. LSEC dysfunction with diminished NO bioavailability can lead to sinusoidal contraction by activated perisinusoidal HSCs and increased IHVR and portal pressure.14 Diminished NO may in turn aggravate liver steatosis and further increase IHVR. NO can decrease liver steatosis by s-nitrosylation of very long chain acyl coenzyme A dehydrogenase, an enzyme in the liver that catalyzes the first committed step in fatty acid β-oxidation.28 NO is also involved in fatty acid synthesis by controlling mitochondria enzymes, such as citrate synthase and NADH–cytochrome c oxidoreductase (KI+III) and inhibition of glycerol-3-phosphate acyltransferase (GPAT).[29.30] In addition to NO, hedgehog signaling, the expression TGFβ and VEGF by dysfunctional LSEC are also increased and augment HSC activation and sinusoidal contraction.31

Change of liver steatosis is associated with splanchnic vasodilatation

Francque et al.32 showed that in Wistar rats fed a methionine choline-deficient diet, steatosis induced portal hypertension with an increase in mesenteric arterial and portal venous flow, arterial low responsiveness to vasoconstrictors, and decreased mean arterial blood pressure, indicating the presence of splanchnic vasodilation and hyperdynamic circulation.32

Extrahepatic factors

Insulin resistance is associated with NAFLD through increased adipose tissue lipolysis, with the increase of free fatty acid delivery to hepatocytes. Insulin resistance may also play a role in LSEC dysfunction and contribute to IHVR and portal pressure. Pasarin et al.33 in a study using a rat model of simple steatosis, showed that dose-dependent sinusoidal endothelium vasodilation was blunted in response to insulin in rats fed an HFD. Treatment with metformin, an insulin sensitizer, restored insulin-enhanced endothelium vasodilatation in the livers of the HFD-fed rats. In their analysis, Francque et al.13 showed that the homeostatic model assessment for insulin resistance (HOMA-IR), as a parameter of insulin resistance, was significantly higher in NAFLD patients with PH than without PH, independent of liver steatosis and visceral fat.13

Gut dysbiosis has a role in NAFLD disease progression, including the development of portal hypertension. In rat model of NASH, 8 weeks of high-fat and high glucose-fructose feeding induced histological NASH without fibrosis, accompanied by endothelial dysfunction and increased portal pressure. In that rat model of NASH, intestinal microbiome diversity was reduced with significant increase in Firmicutes and decrease in Bacteroidetes. Furthermore, fecal transplantation from control rats reduced portal pressure and IHVR and improvement of endothelial dysfunction.34

Animal models

Several animal models have been used to study portal hypertension in NAFLD (Table 1).26,27,34–36 The models show that portal pressure increases early in NAFLD or NASH in the absence of advanced fibrosis or cirrhosis. Unlike common animal models of cirrhotic portal hypertension, the presence of hyperdynamic circulation and increased portal vein inflow were not consistent evident in diet-induced model.

Table 1

NAFLD animal models with portal hypertension

Author (year)AnimalHistopathologyNote
Dietary model
  Francque et al., 201227Male Wistar rats methionine choline-deficient diet for 4–8 weeksSevere steatosis without marked inflammation & fibrosisNo feature of metabolic syndrome. Increased portal pressure
  Pasarin et al., 201226Male Wistar Kyoto rats. Cafeteria diet (65% saturated fat) for 1 monthSteatosis without inflammation and fibrosisWith features of metabolic syndrome. Increased trend of portal pressure (not significant)
  Garcia et al., 201834Male Sprague Dawley rats. High-fat high glucose-fructose diet (30% fat mainly saturated) for 8 weeksSteatohepatitis with mild or absent fibrosisWith features of metabolic syndrome. Increased portal pressure
Transgenic mice
  Klein et al., 201935Transgenic TG (mRen2)27(Ren2) hypertensive rats with elevated tissue Angiotensin IIRenin induced liver injury. Steatohepatitis and mild fibrosisHypertensive rats, nonobese. Renin induced portal hypertension
Combined
  Cremonese et al., 202036TGR (mREN2)27 rats with Western diet for 2 or 4 weeksSteatohepatitis and fibrosisObese. Increased portal pressure

NAFLD, nonalcoholic fatty liver disease.

Clinical aspect

Diagnosis

Currently, hepatic venous pressure gradient (HVPG) is considered the main diagnostic tool for measuring the PPG and is considered the gold standard for measurement of CSPH.37 HVPG is the difference between wedged and free hepatic venous pressure. Limitations of HVPG are invasiveness and availability limited to specialized centers. The reported prevalence of portal hypertension, defined as HVPG >5 mmHg, was lower in nonobese and obese NASH compared with other etiologies of compensated advanced chronic liver disease.8 There was a weaker correlation between wedged hepatic venous pressure and portal pressure in decompensated NASH cirrhosis compared to alcohol or viral-related cirrhosis, suggesting that HVPG might underestimate the portal venous pressure 38 That might be associated with the presence of presinusoidal hypertension or the heterogenous distribution of steatosis and fibrosis within the liver parenchyma. Another study showed that portal hypertension-related decompensation was associated with lower HVPG levels in advanced NAFLD.39 In NASH cirrhosis, a decrease in HVPG after nonselective beta blocker was not predictor of decompensation or long-term transplant free survival.40 Therefore, routine HVPG measurement may not be an ideal tool for detection and monitoring of portal hypertension in NAFLD or different HVPG cutoff level should be established for NAFLD.

Some noninvasive tools have been developed for the detection of CSPH through measurement of liver or spleen tissue stiffness. According to the revised Baveno VII criteria, a combination of liver stiffness measurement by transient elastography (TE) ≤15 kPa and platelet count ≥150×109/L) have sensitivity and negative predictive values of >90% for ruling out CSPH in most etiologies of compensated advanced liver diseases (cALD, TE >10 kPa), including NASH. In viral- and alcohol-related cALD and also in nonobese NASH-related cALD, using a higher cutoff of TE ≥ 25kPa, we can rule in CSPH with specificity and positive predictive value >90%) but not in obese NASH.37 While Baveno VI criteria (TE <20 kPa + platelet count >150×103/mm3) can be used to rule out high-risk esophageal varices.41 However, obesity can overestimate liver stiffness measurement using TE, and obese patients have a lower prevalence of CSPH despite high liver stiffness.37,42

Splenomegaly in portal hypertension is associated with changes in spleen stiffness due to splenic congestion and spleen tissue hyperplasia and fibrosis. Spleen stiffness measurement (SSM) using several techniques such as TE, SWE, or MRE could predict portal hypertension and the presence of esophageal varices or high-risk varices.43 In cALD patients of various etiologies, Colecchia et al.44 showed that the combined model of Baveno VI criteria + SSM ≤46 kPa could rule out high-risk esophageal varices with high sensitivity and negative predictive value. A spleen-dedicated stiffness measurement, using a 100 Hz specific TE probe, has recently been shown to be more accurate for predicting varices or high-risk varices in chronic liver disease,45,46 but further studies are needed to validate the findings in NAFLD/NASH. In early NAFLD/NASH, IHVR may not be accompanied by the splenomegaly and concomitant increase of splanchnic inflow. Therefore, the use of SSM for predicting portal hypertension in NAFLD may be limited to patients with advanced liver disease or cirrhosis.

Several Doppler ultrasound (US) parameters are associated with portal hypertension in chronic liver disease. However, definitive parameters for accurate prediction of portal hypertension in NAFLD are lacking. In NAFLD, steatosis grade is correlated with reduced portal venous blood velocity (lower peak maximum–minimum velocity, mean flow velocity, and portal vein pulsatility index (VPI) and compensatory increase of hepatic arterial flow (lower hepatic artery resistive index).15,16,47,48 Using routine Doppler US, portal VPI can be calculated as (Vmax – Vmin) / Vmax, where Vmax is the maximum and Vmin is the minimum pulsed-wave Doppler ultrasound–estimated velocity of portal venous blood. Baikpour et al.15 showed that lower portal vein pulsatility index (VPI) was associated with higher fibrosis stage in NAFLD and could be used to predict high-risk NAFLD (with ≥F2 stage liver fibrosis). Further study of VPI for predicting and monitoring portal hypertension in NAFLD is still needed.

Several novel methods have been developed to diagnose portal hypertension, such as subharmonic aided pressure estimation (SHAPE) using contrast enhanced ultrasonography, magnetic resonance (MR) methods, and the endoscopic ultrasound-portal pressure gradient (EUS-PPG). Gupta et al.49 compared SHAPE to HVPG to diagnose portal hypertension. In 125 patients, 18% with NASH and most with chronic hepatitis C, they found that SHAPE had 95% accuracy for detecting CSPH or HVPG ≥12 mmHg.49 MR elastography (MRE) has been studied in NAFLD. A multicenter retrospective study found that liver stiffness assessed by MRE had an area under the curve of 0.707 (95% CI: 0.511–0.902) for differentiating decompensated NAFLD-related cirrhosis, defined as ascites, hepatic encephalopathy or esophageal variceal bleeding, from compensated cirrhosis.50 Recently, EUS-guided direct measurement of portal vein and hepatic vein pressure under the guidance has been developed. The mean difference of portal vein and hepatic vein pressure is then reported as the PPG. A human pilot study showed excellent correlation between EUS-PPG and HVPG (r=0.923).51 The potential use of EUS-PPG for monitoring portal hypertension after endoscopic gastric plication in NASH-related cirrhosis has also been reported.52

Endothelial dysfunction contributes to intrahepatic resistance in NAFLD, and von Willebrand factor (VWF-Ag) is considered a marker of endothelial dysfunction. A retrospective study of 236 cirrhosis patients with various etiologies including NASH (12.3%) evaluated the diagnostic performance of VWF-Ag for predicting CSPH. At a cutoff of >226, VWF-Ag had an area under the curve (AUC) of 0.79 (95% CI: 0.72–0.87), a sensitivity of 76%, and a specificity of 71%. Using the VWF-Ag/thrombocyte ratio (VITRO score), there was the AUC increased to 0.86 (95% CI: 0.81–0.91), with a sensitivity of 80% and a specificity of 70% at a cutoff of >1.58 in predicting CSPH. The combination of TE and VITRO score further improved prediction for CSPH, with an AUC of 0.96 (95% CI: 0.91–0.98), a sensitivity of 91%, and a specificity of 93% at a cutoff of >0.71.53

Therapy

Lifestyle interventions including diet and physical exercise to reduce body weight is considered the first-line treatment for obese NAFLD. A target of 7–10% weight loss is associated with improvement in liver histology, including fibrosis. Obesity is a predictor of decompensation in compensated cirrhotic patients with various etiologies independent of HVPG and albumin or treatment with a beta blocker.54 In the SportDiet study, a pilot study involving overweight/obese patients with compensated cirrhosis and an HVPG ≥ 6 mmHg, Berzigotti et al.55 showed that a 16 week individualized hypocaloric normal-protein diet and 60 min/week of moderate exercise significantly reduced HVPG by 10–20%. The reduced HVPG was significantly associated with body weight loss. There were no episodes of decompensation during the short-term intervention, suggesting that calorie restriction while maintaining protein intake with moderate exercise is safe in compensated cirrhosis. Further study is needed to evaluate the long-term efficacy, feasibility, and safety of lifestyle interventions in cALD or cirrhosis.

Nonselective beta blockers (NSBBs) like propranolol, nadolol, or carvedilol are recommended in compensated cirrhosis with CSPH to prevent clinical decompensation and improve survival. NSBB reduces the risk of variceal bleeding not only by reducing the portal pressure, but also by improving intestinal permeability and reduced bacterial translocation.56 Carvedilol is the preferred NSBB in compensated cirrhosis because of a greater reduction of portal pressure. Carvedilol does not adversely affect insulin sensitivity, glucose and lipid profile, which should be considered in NAFLD patients with metabolic comorbidities.57

Statin use is common in NAFLD with dyslipidemia or high cardiovascular risk. Several nonrandomized, controlled studies suggest beneficial effect of statin on NASH resolution and liver fibrosis. In an early NASH model with portal hypertension, statins decreased portal pressure by inducing recovery of LSEC capillarization and regression of HSC activation by upregulation of the hepatic endothelial expression of Kruppel-like factor 2, an endothelial transcription factor. Statins also decrease hepatic stellate cell activation by inhibition of RhoA / Rho-kinase signaling.58 A study by Zafra et al.59 showed that acute administration of 40 mg simvastatin in cirrhotic patients increased hepatic blood flow, decreased hepatic resistance, and increased NO products in hepatic venous blood.59 That proof-of-concept study was followed by several randomized controlled trials (RCTs) that evaluated the effect of statins on HVPG levels. A meta-analysis of six RCTs showed that statin use was associated with reduction of portal hypertension >20% of baseline or <12 mmHg) at 1 month (RR=2.01; 95% CI: 1.31–3.10) but the difference was not significant at 3 months (RR=3.76; 95% CI: 0.36–39.77).60 None of the studies specifically looked at NAFLD-related cirrhosis. Because of potential liver and muscle toxicity, we should be cautious on the use of statins in decompensated cirrhosis and it should be avoided Child-Pugh C patients.

Obeticholic acid, a farnesoid X receptor agonist, has been shown to improve the histological features of NASH.61 In a rat model of cirrhotic portal hypertension, it lowered IHVR associated with increased eNOS activity, down-regulation of Rho-kinase, upregulation of dimethylarginine dimethylaminohydrolase-2, and reduced asymmetric-dimethylarginine, an eNOS inhibitor.62,63

Sodium-glucose transport protein 2 (SGLT2) inhibitors are a class of antidiabetic agents that have also been studied in NAFLD or NASH. SGLT2 inhibitors reduce transaminase levels and improve liver fat content and body composition in NAFLD patients with type 2 diabetes mellitus.64 SGLT2 inhibitors target the pathophysiology of portal hypertension. SGLT2 blockade inhibits glucose and sodium reabsorption in the proximal renal tubule, resulting in an increase in sodium delivery to the macula densa. Consequently, both renin secretion and angiotensin II level are reduced. SGLT2 inhibitors also reduce sympathetic nervous activity.65 A recent observational study showed that SGLT2 inhibitors were well tolerated in a small sample of cirrhotic patients and type 2 DM.66 Further studies are needed to evaluate the therapeutic effectiveness of SGLT2 inhibitors in portal hypertension.

The renin angiotensin system (RAS) is associated with disease progression in NAFLD. Experimental data has indicated that angiotensin II (Ang II) generation was associated with de novo lipogenesis, mitochondrial dysfunction, reactive oxygen species generation, pro-inflammatory cytokine production, and HSC activation.67 Steatohepatitis with portal hypertension developed in transgenic TGR (mREN2)27 rats overexpressing mouse renin. Stimulation of angiotensin II type 1 receptor in HSCs by Ang II was found to induce fibrosis and portal hypertension via Janus kinase-2.35,36 Clinical evidence of the therapeutic effectiveness of RAS inhibitors on the development of portal hypertension in NAFLD is still limited. However, several observational studies found a significant association of RAS inhibitors and disease regression in NAFLD patients with obesity or type 2 diabetes.68,69

Conclusion

Experimental and clinical evidence suggests that portal hypertension develop early in NAFLD through an increase in IHVR. Both the mechanical component of liver steatosis and the molecular component of liver sinusoidal endothelial dysfunction together with insulin resistance and gut dysbiosis in NAFLD augment IHVR. Furthermore, liver sinusoidal endothelial dysfunction activates HSCs and further augments IHVR via perisinusoidal fibrosis (Fig. 1). HVPG is recommended as the gold standard for diagnosing portal hypertension, but in advanced NAFLD, HVPG might underestimate portal pressure, and decompensation can still occur in a small number of patients with mild HVPG. Noninvasive liver stiffness measurement is considered an alternative to predict CSPH in NAFLD, but obesity might obscure the impact of liver stiffness on portal venous pressure. Further validation is needed for new biomarkers or other noninvasive tools specifically in NAFLD. Lifestyle modification is the first-line therapy for NAFLD and also to control comorbidities, with early data suggesting that body weight loss is also associated with reduction of HVPG levels. Similar to other etiologies, NSBB is also recommended to prevent decompensation and improve survival in NAFLD with CSPH, and statins may decrease portal venous pressure in addition to improvement of dyslipidemia and cardiovascular risk in NAFLD. Future drug development for NAFLD should also address portal hypertension as an important endpoint.

Theoretical framework of portal hypertension in NAFLD.
Fig. 1  Theoretical framework of portal hypertension in NAFLD.

LSEC, liver sinusoidal endothelial cell; HSC, hepatic stellate cell; NAFLD, nonalcoholic fatty liver disease.

Abbreviations

Ang II: 

angiotensin II

cALD: 

compensated advance liver disease

CSPH: 

clinically significant portal hypertension

eNOS: 

endothelial nitric oxide synthase

EUS-PPG: 

endoscopic ultrasound-portal pressure gradient

HFD: 

high-fat diet

HCC: 

hepatocellular carcinoma

HSC: 

hepatic stellate cells

HVPG: 

hepatic venous pressure gradient

IHVR: 

intrahepatic vascular resistance

LSEC: 

liver sinusoidal endothelial dysfunction

MAFLD: 

metabolic associated fatty liver disease

MRE: 

magnetic resonance elastography

NAFLD: 

nonalcoholic fatty liver disease

NASH: 

nonalcoholic steatohepatitis

NO: 

nitric oxide

NSBB: 

nonselective beta blocker

RCT: 

randomized controlled trial

SGLT2: 

sodium-glucose transport protein 2

SHAPE: 

Subharmonic aided pressure estimation

SSM: 

spleen stiffness measurement

SWE: 

shear wave elastography

TE: 

transient elastography

VPI: 

portal vein pulsatility index

vWF: 

von Willebrand factor

Declarations

Funding

None to declare.

Conflict of interest

The authors have no conflict of interests related to this publication.

Authors’ contributions

Study concept and design (CRAL), analysis and interpretation of data (SHHN, CRAL), drafting of the manuscript (SHHN, CRAL), critical revision of the manuscript for important intellectual content (SHHN, CRAL), and administrative, technical, or material support, study supervision (CRAL).

References

  1. Younossi ZM, Koenig AB, Abdelatif D, Fazel Y, Henry L, Wymer M. Global epidemiology of nonalcoholic fatty liver disease-Meta-analytic assessment of prevalence, incidence, and outcomes. Hepatology 2016;64(1):73-84 View Article PubMed/NCBI
  2. Li J, Zou B, Yeo YH, Feng Y, Xie X, Lee DH, et al. Prevalence, incidence, and outcome of non-alcoholic fatty liver disease in Asia, 1999-2019: a systematic review and meta-analysis. Lancet Gastroenterol Hepatol 2019;4(5):389-398 View Article PubMed/NCBI
  3. Lesmana CR, Pakasi LS, Inggriani S, Aidawati ML, Lesmana LA. Development of non-alcoholic fatty liver disease scoring system among adult medical check-up patients: a large cross-sectional and prospective validation study. Diabetes Metab Syndr Obes 2015;8:213-218 View Article PubMed/NCBI
  4. Liu J, Ayada I, Zhang X, Wang L, Li Y, Wen T, et al. Estimating Global Prevalence of Metabolic Dysfunction-Associated Fatty Liver Disease in Overweight or Obese Adults. Clin Gastroenterol Hepatol 2022;20(3):e573-e582 View Article PubMed/NCBI
  5. Simon TG, Roelstraete B, Khalili H, Hagström H, Ludvigsson JF. Mortality in biopsy-confirmed nonalcoholic fatty liver disease: results from a nationwide cohort. Gut 2021;70(7):1375-1382 View Article PubMed/NCBI
  6. Kabbany MN, Conjeevaram Selvakumar PK, Watt K, Lopez R, Akras Z, Zein N, et al. Prevalence of Nonalcoholic Steatohepatitis-Associated Cirrhosis in the United States: An Analysis of National Health and Nutrition Examination Survey Data. Am J Gastroenterol 2017;112(4):581-587 View Article PubMed/NCBI
  7. Wong RJ, Singal AK. Trends in Liver Disease Etiology Among Adults Awaiting Liver Transplantation in the United States, 2014-2019. JAMA Netw Open 2020;3(2):e1920294 View Article PubMed/NCBI
  8. Pons M, Augustin S, Scheiner B, Guillaume M, Rosselli M, Rodrigues SG, et al. Noninvasive Diagnosis of Portal Hypertension in Patients With Compensated Advanced Chronic Liver Disease. Am J Gastroenterol 2021;116(4):723-732 View Article PubMed/NCBI
  9. Mendes FD, Suzuki A, Sanderson SO, Lindor KD, Angulo P. Prevalence and indicators of portal hypertension in patients with nonalcoholic fatty liver disease. Clin Gastroenterol Hepatol 2012;10(9):1028-33.e2 View Article PubMed/NCBI
  10. Rodrigues SG, Montani M, Guixé-Muntet S, De Gottardi A, Berzigotti A, Bosch J. Patients With Signs of Advanced Liver Disease and Clinically Significant Portal Hypertension Do Not Necessarily Have Cirrhosis. Clin Gastroenterol Hepatol 2019;17(10):2101-2109.e1 View Article PubMed/NCBI
  11. Francque S, Verrijken A, Mertens I, Hubens G, Van Marck E, Pelckmans P, et al. Noncirrhotic human nonalcoholic fatty liver disease induces portal hypertension in relation to the histological degree of steatosis. Eur J Gastroenterol Hepatol 2010;22(12):1449-1457 View Article PubMed/NCBI
  12. Vonghia L, Magrone T, Verrijken A, Michielsen P, Van Gaal L, Jirillo E, et al. Peripheral and Hepatic Vein Cytokine Levels in Correlation with Non-Alcoholic Fatty Liver Disease (NAFLD)-Related Metabolic, Histological, and Haemodynamic Features. PLoS One 2015;10(11):e0143380 View Article PubMed/NCBI
  13. Francque S, Verrijken A, Mertens I, Hubens G, Van Marck E, Pelckmans P, et al. Visceral adiposity and insulin resistance are independent predictors of the presence of non-cirrhotic NAFLD-related portal hypertension. Int J Obes (Lond) 2011;35(2):270-278 View Article PubMed/NCBI
  14. Fernandez M. Molecular pathophysiology of portal hypertension. Hepatology 2015;61(4):1406-1415 View Article PubMed/NCBI
  15. Baikpour M, Ozturk A, Dhyani M, Mercaldo ND, Pierce TT, Grajo JR, et al. Portal Venous Pulsatility Index: A Novel Biomarker for Diagnosis of High-Risk Nonalcoholic Fatty Liver Disease. AJR Am J Roentgenol 2020;214(4):786-791 View Article PubMed/NCBI
  16. Balci A, Karazincir S, Sumbas H, Oter Y, Egilmez E, Inandi T. Effects of diffuse fatty infiltration of the liver on portal vein flow hemodynamics. J Clin Ultrasound 2008;36(3):134-140 View Article PubMed/NCBI
  17. Erdogmus B, Tamer A, Buyukkaya R, Yazici B, Buyukkaya A, Korkut E, et al. Portal vein hemodynamics in patients with non-alcoholic fatty liver disease. Tohoku J Exp Med 2008;215(1):89-93 View Article PubMed/NCBI
  18. Davis RP, Surewaard BGJ, Turk M, Carestia A, Lee WY, Petri B, et al. Optimization of In vivo Imaging Provides a First Look at Mouse Model of Non-Alcoholic Fatty Liver Disease (NAFLD) Using Intravital Microscopy. Front Immunol 2019;10:2988 View Article PubMed/NCBI
  19. McCuskey RS, Ito Y, Robertson GR, McCuskey MK, Perry M, Farrell GC. Hepatic microvascular dysfunction during evolution of dietary steatohepatitis in mice. Hepatology 2004;40(2):386-393 View Article PubMed/NCBI
  20. Miyao M, Kotani H, Ishida T, Kawai C, Manabe S, Abiru H, et al. Pivotal role of liver sinusoidal endothelial cells in NAFLD/NASH progression. Lab Invest 2015;95(10):1130-1144 View Article PubMed/NCBI
  21. Akyol G, Erdem O, Yilmaz G. Nonalcoholic fatty liver disease. Correlation with histology and viral hepatitis. Saudi Med J 2005;26(12):1904-1910 PubMed/NCBI
  22. Zhang Q, Liu J, Liu J, Huang W, Tian L, Quan J, et al. oxLDL induces injury and defenestration of human liver sinusoidal endothelial cells via LOX1. J Mol Endocrinol 2014;53(2):281-293 View Article PubMed/NCBI
  23. Herrnberger L, Hennig R, Kremer W, Hellerbrand C, Goepferich A, Kalbitzer HR, et al. Formation of fenestrae in murine liver sinusoids depends on plasmalemma vesicle-associated protein and is required for lipoprotein passage. PLoS One 2014;9(12):e115005 View Article PubMed/NCBI
  24. Bravo M, Raurell I, Hide D, Fernández-Iglesias A, Gil M, Barberá A, et al. Restoration of liver sinusoidal cell phenotypes by statins improves portal hypertension and histology in rats with NASH. Sci Rep 2019;9(1):20183 View Article PubMed/NCBI
  25. Gonzalez-Paredes FJ, Hernández Mesa G, Morales Arraez D, Marcelino Reyes R, Abrante B, Diaz-Flores F, et al. Contribution of Cyclooxygenase End Products and Oxidative Stress to Intrahepatic Endothelial Dysfunction in Early Non-Alcoholic Fatty Liver Disease. PLoS One 2016;11(5):e0156650 View Article PubMed/NCBI
  26. Pasarín M, La Mura V, Gracia-Sancho J, García-Calderó H, Rodríguez-Vilarrupla A, García-Pagán JC, et al. Sinusoidal endothelial dysfunction precedes inflammation and fibrosis in a model of NAFLD. PLoS One 2012;7(4):e32785 View Article PubMed/NCBI
  27. Francque S, Laleman W, Verbeke L, Van Steenkiste C, Casteleyn C, Kwanten W, et al. Increased intrahepatic resistance in severe steatosis: endothelial dysfunction, vasoconstrictor overproduction and altered microvascular architecture. Lab Invest 2012;92(10):1428-1439 View Article PubMed/NCBI
  28. Doulias PT, Tenopoulou M, Greene JL, Raju K, Ischiropoulos H. Nitric oxide regulates mitochondrial fatty acid metabolism through reversible protein S-nitrosylation. Sci Signal 2013;6(256):rs1 View Article PubMed/NCBI
  29. Schild L, Dombrowski F, Lendeckel U, Schulz C, Gardemann A, Keilhoff G. Impairment of endothelial nitric oxide synthase causes abnormal fat and glycogen deposition in liver. Biochim Biophys Acta 2008;1782(3):180-187 View Article PubMed/NCBI
  30. Park H, Kaushik VK, Constant S, Prentki M, Przybytkowski E, Ruderman NB, et al. Coordinate regulation of malonyl-CoA decarboxylase, sn-glycerol-3-phosphate acyltransferase, and acetyl-CoA carboxylase by AMP-activated protein kinase in rat tissues in response to exercise. J Biol Chem 2002;277(36):32571-32577 View Article PubMed/NCBI
  31. Wilkinson AL, Qurashi M, Shetty S. The Role of Sinusoidal Endothelial Cells in the Axis of Inflammation and Cancer Within the Liver. Front Physiol 2020;11:990 View Article PubMed/NCBI
  32. Francque S, Wamutu S, Chatterjee S, Van Marck E, Herman A, Ramon A, et al. Non-alcoholic steatohepatitis induces non-fibrosis-related portal hypertension associated with splanchnic vasodilation and signs of a hyperdynamic circulation in vitro and in vivo in a rat model. Liver Int 2010;30(3):365-375 View Article PubMed/NCBI
  33. Pasarín M, Abraldes JG, Rodríguez-Vilarrupla A, La Mura V, García-Pagán JC, Bosch J. Insulin resistance and liver microcirculation in a rat model of early NAFLD. J Hepatol 2011;55(5):1095-1102 View Article PubMed/NCBI
  34. García-Lezana T, Raurell I, Bravo M, Torres-Arauz M, Salcedo MT, Santiago A, et al. Restoration of a healthy intestinal microbiota normalizes portal hypertension in a rat model of nonalcoholic steatohepatitis. Hepatology 2018;67(4):1485-1498 View Article PubMed/NCBI
  35. Klein S, Kleine CE, Pieper A, Granzow M, Gautsch S, Himmit M, et al. TGR(mREN2)27 rats develop non-alcoholic fatty liver disease-associated portal hypertension responsive to modulations of Janus-kinase 2 and Mas receptor. Sci Rep 2019;9(1):11598 View Article PubMed/NCBI
  36. Cremonese C, Schierwagen R, Uschner FE, Torres S, Tyc O, Ortiz C, et al. Short-Term Western Diet Aggravates Non-Alcoholic Fatty Liver Disease (NAFLD) With Portal Hypertension in TGR (mREN2)27 Rats. Int J Mol Sci 2020;21(9):E3308 View Article PubMed/NCBI
  37. de Franchis R, Bosch J, Garcia-Tsao G, Reiberger T, Ripoll C, Baveno VII Faculty. Baveno VII - Renewing consensus in portal hypertension. J Hepatol 2022;76(4):959-974 View Article PubMed/NCBI
  38. Ferrusquía-Acosta J, Bassegoda O, Turco L, Reverter E, Pellone M, Bianchini M, et al. Agreement between wedged hepatic venous pressure and portal pressure in non-alcoholic steatohepatitis-related cirrhosis. J Hepatol 2021;74(4):811-818 View Article PubMed/NCBI
  39. Bassegoda O, Olivas P, Turco L, Mandorfer M, Serra-Burriel M, Tellez L, et al. Decompensation in Advanced Nonalcoholic Fatty Liver Disease May Occur at Lower Hepatic Venous Pressure Gradient Levels Than in Patients With Viral Disease. Clin Gastroenterol Hepatol 2021 View Article PubMed/NCBI
  40. Paternostro R, Becker J, Hofer BS, Panagl V, Schiffke H, Simbrunner B, et al. The prognostic value of HVPG-response to non-selective beta-blockers in patients with NASH cirrhosis and varices. Dig Liver Dis 2022;54(4):500-508 View Article PubMed/NCBI
  41. Galizzi HO, Couto CA, Taranto DOL, Araújo SIO, Vilela EG. Accuracy of non-invasive methods/models for predicting esophageal varices in patients with compensated advanced chronic liver disease secondary to nonalcoholic fatty liver disease. Ann Hepatol 2021;20:100229 View Article PubMed/NCBI
  42. Wong VW, Irles M, Wong GL, Shili S, Chan AW, Merrouche W, et al. Unified interpretation of liver stiffness measurement by M and XL probes in non-alcoholic fatty liver disease. Gut 2019;68(11):2057-2064 View Article PubMed/NCBI
  43. Hu X, Huang X, Hou J, Ding L, Su C, Meng F. Diagnostic accuracy of spleen stiffness to evaluate portal hypertension and esophageal varices in chronic liver disease: a systematic review and meta-analysis. Eur Radiol 2021;31(4):2392-2404 View Article PubMed/NCBI
  44. Colecchia A, Ravaioli F, Marasco G, Colli A, Dajti E, Di Biase AR, et al. A combined model based on spleen stiffness measurement and Baveno VI criteria to rule out high-risk varices in advanced chronic liver disease. J Hepatol 2018;69(2):308-317 View Article PubMed/NCBI
  45. Nagai K, Ogawa Y, Kobayashi T, Iwaki M, Nogami A, Honda Y, et al. Gastroesophageal varices evaluation using spleen-dedicated stiffness measurement by vibration-controlled transient elastography. JGH Open 2022;6(1):11-19 View Article PubMed/NCBI
  46. Stefanescu H, Marasco G, Calès P, Fraquelli M, Rosselli M, Ganne-Carriè N, et al. A novel spleen-dedicated stiffness measurement by FibroScan® improves the screening of high-risk oesophageal varices. Liver Int 2020;40(1):175-185 View Article PubMed/NCBI
  47. Sabry M, Youssef T, Shaker M, Salama MM, Assem N, Anwar CA. Portal venous and hepatic artery hemodynamic variation in non-alcoholic fatty liver disease. Egypt Liver Journal 2021;11:58 View Article
  48. Balasubramanian P, Boopathy V, Govindasamy E, Venkatesh BP. Assessment of Portal Venous and Hepatic Artery Haemodynamic Variation in Non-Alcoholic Fatty Liver Disease (NAFLD) Patients. J Clin Diagn Res 2016;10(8):TC07-TC10 View Article PubMed/NCBI
  49. Gupta I, Eisenbrey JR, Machado P, Stanczak M, Wessner CE, Shaw CM, et al. Diagnosing Portal Hypertension with Noninvasive Subharmonic Pressure Estimates from a US Contrast Agent. Radiology 2021;298(1):104-111 View Article PubMed/NCBI
  50. Han MAT, Vipani A, Noureddin N, Ramirez K, Gornbein J, Saouaf R, et al. MR elastography-based liver fibrosis correlates with liver events in nonalcoholic fatty liver patients: A multicenter study. Liver Int 2020;40(9):2242-2251 View Article PubMed/NCBI
  51. Zhang W, Peng C, Zhang S, Huang S, Shen S, Xu G, et al. EUS-guided portal pressure gradient measurement in patients with acute or subacute portal hypertension. Gastrointest Endosc 2021;93(3):565-572 View Article PubMed/NCBI
  52. Jirapinyo P, Thompson CC, Ryou M. Effects of endoscopic gastric plication on portal pressure gradient in a patient with nonalcoholic steatohepatitis cirrhosis. VideoGIE 2021;6(11):491-494 View Article PubMed/NCBI
  53. Hametner S, Ferlitsch A, Ferlitsch M, Etschmaier A, Schöfl R, Ziachehabi A, et al. The VITRO Score (Von Willebrand Factor Antigen/Thrombocyte Ratio) as a New Marker for Clinically Significant Portal Hypertension in Comparison to Other Non-Invasive Parameters of Fibrosis Including ELF Test. PLoS One 2016;11(2):e0149230 View Article PubMed/NCBI
  54. Berzigotti A, Garcia-Tsao G, Bosch J, Grace ND, Burroughs AK, Morillas R, et al. Obesity is an independent risk factor for clinical decompensation in patients with cirrhosis. Hepatology 2011;54(2):555-561 View Article PubMed/NCBI
  55. Berzigotti A, Albillos A, Villanueva C, Genescá J, Ardevol A, Augustín S, et al. Effects of an intensive lifestyle intervention program on portal hypertension in patients with cirrhosis and obesity: The SportDiet study. Hepatology 2017;65(4):1293-1305 View Article PubMed/NCBI
  56. Reiberger T, Ferlitsch A, Payer BA, Mandorfer M, Heinisch BB, Hayden H, et al. Non-selective betablocker therapy decreases intestinal permeability and serum levels of LBP and IL-6 in patients with cirrhosis. J Hepatol 2013;58(5):911-921 View Article PubMed/NCBI
  57. Deedwania P. Hypertension, dyslipidemia, and insulin resistance in patients with diabetes mellitus or the cardiometabolic syndrome: benefits of vasodilating β-blockers. J Clin Hypertens (Greenwich) 2011;13(1):52-59 View Article PubMed/NCBI
  58. Bosch J, Gracia-Sancho J, Abraldes JG. Cirrhosis as new indication for statins. Gut 2020;69(5):953-962 View Article PubMed/NCBI
  59. Zafra C, Abraldes JG, Turnes J, Berzigotti A, Fernández M, Garca-Pagán JC, et al. Simvastatin enhances hepatic nitric oxide production and decreases the hepatic vascular tone in patients with cirrhosis. Gastroenterology 2004;126(3):749-755 View Article PubMed/NCBI
  60. Wan S, Huang C, Zhu X. Systematic review with a meta-analysis: clinical effects of statins on the reduction of portal hypertension and variceal haemorrhage in cirrhotic patients. BMJ Open 2019;9(7):e030038 View Article PubMed/NCBI
  61. Younossi ZM, Ratziu V, Loomba R, Rinella M, Anstee QM, Goodman Z, et al. Obeticholic acid for the treatment of non-alcoholic steatohepatitis: interim analysis from a multicentre, randomised, placebo-controlled phase 3 trial. Lancet 2019;394(10215):2184-2196 View Article PubMed/NCBI
  62. Mookerjee RP, Mehta G, Balasubramaniyan V, Mohamed Fel Z, Davies N, Sharma V, et al. Hepatic dimethylarginine-dimethylaminohydrolase1 is reduced in cirrhosis and is a target for therapy in portal hypertension. J Hepatol 2015;62(2):325-331 View Article PubMed/NCBI
  63. Verbeke L, Farre R, Trebicka J, Komuta M, Roskams T, Klein S, et al. Obeticholic acid, a farnesoid X receptor agonist, improves portal hypertension by two distinct pathways in cirrhotic rats. Hepatology 2014;59(6):2286-2298 View Article PubMed/NCBI
  64. Wei Q, Xu X, Guo L, Li J, Li L. Effect of SGLT2 Inhibitors on Type 2 Diabetes Mellitus With Non-Alcoholic Fatty Liver Disease: A Meta-Analysis of Randomized Controlled Trials. Front Endocrinol (Lausanne) 2021;12:635556 View Article PubMed/NCBI
  65. Wan N, Rahman A, Hitomi H, Nishiyama A. The Effects of Sodium-Glucose Cotransporter 2 Inhibitors on Sympathetic Nervous Activity. Front Endocrinol (Lausanne) 2018;9:421 View Article PubMed/NCBI
  66. Saffo S, Garcia-Tsao G, Taddei T. SGLT2 inhibitors in patients with cirrhosis and diabetes mellitus: A tertiary center cohort study and insights about a potential therapeutic target for portal hypertension. J Diabetes 2021;13(3):265-269 View Article PubMed/NCBI
  67. Simões E Silva AC, Miranda AS, Rocha NP, Teixeira AL. Renin angiotensin system in liver diseases: Friend or foe?. World J Gastroenterol 2017;23(19):3396-3406 View Article PubMed/NCBI
  68. Pelusi S, Petta S, Rosso C, Borroni V, Fracanzani AL, Dongiovanni P, et al. Renin-Angiotensin System Inhibitors, Type 2 Diabetes and Fibrosis Progression: An Observational Study in Patients with Nonalcoholic Fatty Liver Disease. PLoS One 2016;11(9):e0163069 View Article PubMed/NCBI
  69. Kim KM, Roh JH, Lee S, Yoon JH. Clinical implications of renin-angiotensin system inhibitors for development and progression of non-alcoholic fatty liver disease. Sci Rep 2021;11(1):2884 View Article PubMed/NCBI
Copyright © 2022 Authors. This is an Open Access article distributed under the terms of the Creative Commons Attribution-Noncommercial 4.0 License (CC BY-NC 4.0), permitting all non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.
  • Journal of Clinical and Translational Hepatology
  • pISSN 2225-0719
  • eISSN 2310-8819

Article Options

Metrics

Cite this Article

  • © 2024 Xia & He Publishing Inc.
  • Total visits : 2637499
  • Visits today : 3492
Back to Top

Portal Hypertension in Nonalcoholic Fatty Liver Disease: From Pathogenesis to Clinical Practice

Saut Horas H. Nababan, Cosmas Rinaldi Adithya Lesmana
  • Reset Zoom
  • Download TIFF