Corrigendum on: Wang X, Chen S, Fan J, Gong Y, Liu H, Wang L, et al. Mitochondrial Membrane Potential of CD8+ T Cells Predicts Bacterial Infection and Rapid Development of Acute-on-chronic Liver Failure in Cirrhotic Patients. J Clin Transl Hepatol 2025;13(5):395–408. doi: 10.14218/JCTH.2024.00452.
Text Correction
The original version of the article contained a description error in the Supplementary File 1. Supplemental methods, 1.4 Western Blot. It should read as:
1.4 Western Blot
For the analysis of mitochondrial proteins, organelles were first isolated from CD8+ T cells using the [Cell Mitochondria Isolation Kit, C3601, Beyotime, Shanghai, China] according to the manufacturer's instructions. Western Blot analysis was then performed on lysates from these purified mitochondrial fractions. Total protein was extracted from the cells mitochondria, added to 5× SDS loading buffer, and boiled at 100°C for 10 minutes. The separation glue was prepared as needed, ethanol was added, and the mixture was allowed to stand for 20 minutes. The separation glue was allowed to solidify, the ethanol was removed, the remaining ethanol was rinsed with water, and the residual liquid was removed with absorbent paper. The prepared glue was added, the comb was inserted, the mixture was allowed to sit for 20 minutes, and the comb was removed when the glue solidified. The electrophoresis device was installed at 20 V for 20 minutes, 80 V for 20 minutes, and 120 V for 70 minutes. The wet transfer film was a 0.3-A constant-flow film, exposed for 45 minutes. The PVDF membrane was placed in 5% skim milk and blocked for 2 hours. The membrane was washed with TBST 3 times for 10 minutes each. Primary antibodies (anti-SQSTM1/p62 rabbit mAb, A19700, anti-Parkin rabbit pAb, A0968, anti-PINK1 rabbit pAb, A24745, anti-LC3B rabbit mAb, A19665, ABclonal Technology®, Wuhan, China; anti-VDAC1/porin antibody, ab306581, Abcam, UK; antibody diluted to the appropriate proportions) were incubated with the membranes overnight on a shaking bed at 4°C. The membrane was washed with TBST 3 times for 10 minutes each. The membranes were incubated with a secondary antibody (HRP-conjugated goat anti-rabbit IgG (H+L), AS014, ABclonal Technology®, Wuhan, China; antibody diluent diluted to appropriate proportions) at room temperature for 2 hours. The membrane was washed with TBST 3 times for 10 minutes each. Luminant liquid A and liquid B were added to the kit at a ratio of 1:1, and the mixture was incubated for 1 minute. After exposure, the cells were observed.
Instead of:
1.4 Western Blot
Total protein was extracted from the cells, added to 5× SDS loading buffer, and boiled at 100°C for 10 minutes. The separation glue was prepared as needed, ethanol was added, and the mixture was allowed to stand for 20 minutes. The separation glue was allowed to solidify, the ethanol was removed, the remaining ethanol was rinsed with water, and the residual liquid was removed with absorbent paper. The prepared glue was added, the comb was inserted, the mixture was allowed to sit for 20 minutes, and the comb was removed when the glue solidified. The electrophoresis device was installed at 20 V for 20 minutes, 80 V for 20 minutes, and 120 V for 70 minutes. The wet transfer film was a 0.3-A constant-flow film, exposed for 45 minutes. The PVDF membrane was placed in 5% skim milk and blocked for 2 hours. The membrane was washed with TBST 3 times for 10 minutes each. Primary antibodies (anti-SQSTM1/p62 rabbit mAb, A19700, anti-Parkin rabbit pAb, A0968, anti-PINK1 rabbit pAb, A24745, anti-LC3B rabbit mAb, A19665, ABclonal Technology®, Wuhan, China; anti-VDAC1/porin antibody, ab306581, Abcam, UK; antibody diluted to the appropriate proportions) were incubated with the membranes overnight on a shaking bed at 4°C. The membrane was washed with TBST 3 times for 10 minutes each. The membranes were incubated with a secondary antibody (HRP-conjugated rabbit anti-goat IgG (H+L), AS029, ABclonal Technology®, Wuhan, China; antibody diluent diluted to appropriate proportions) at room temperature for 2 hours. The membrane was washed with TBST 3 times for 10 minutes each. Luminant liquid A and liquid B were added to the kit at a ratio of 1:1, and the mixture was incubated for 1 minute. After exposure, the cells were observed.
The authors apologize for this error and state that the update does not change the results and conclusions originally reported.