v
Search
Advanced

Publications > Journals > Oncology Advances> Article Full Text

  • Review Article
  • OPEN ACCESS

The Tumor Microenvironment and Tumor-infiltrating Lymphocytes in Solid Tumor: A Comprehensive Review

  • Zhuoya Sun,
  • Lupeng Qiu,
  • Xiao Zhao*  and
  • Shunchang Jiao* 
 Author information 

Abstract

The tumor microenvironment is a dynamic cellular landscape critical to cancer progression. Within it, tumor-infiltrating lymphocytes hold a dual role, contributing to both tumor suppression and progression. This review synthesized current knowledge on tumor-infiltrating lymphocytes, emphasizing their prognostic significance and therapeutic potential. By dissecting their interactions within the tumor microenvironment and with cancer cells, we sought to uncover the complexities of the immune response in cancer and explored the future direction of immunotherapeutic strategies.

Keywords

Tumor-infiltrating lymphocytes, Tumor microenvironment, Immunotherapy, Cellular interactions, Prognostic value, Therapeutic targeting.

Introduction

The tumor microenvironment (TME) is a complex and dynamic network essential to the pathogenesis of cancer. It encompasses a diverse array of cell types, including cancer cells, immune cells, stromal cells, and extracellular matrix components,1 all of which collectively contribute to the progression or suppression of cancer. Within this intricate network, tumor-infiltrating lymphocytes (TILs) play a crucial role in the host’s immune defense against cancer. TILs comprise a heterogeneous array of T cells, B cells, and natural killer cells (NKs).2 However, their functionality and efficacy in targeting tumor cells are profoundly modulated by the intricate interactions within the TME.

Beyond their established role in prognostic evaluation, TILs have emerged as promising targets for immunotherapy. The seminal work by Rosenberg et al. in isolating and expanding these lymphocytes from tumor tissues has marked a significant milestone in cancer treatment.3,4 Nonetheless, despite extensive research underscoring the importance of TILs in the oncogenesis and progression of tumors, ambiguities remain regarding the precise cellular makeup, the spatiotemporal dynamics within the TME, and the exact mechanisms by which TIL-based therapies modulate tumor immunity.

This review sought to elucidate the intricate interplay between the adaptive and innate immune components within the TME, with a particular focus on the T and B cell interactions central to the adaptability of the host’s immune response. By consolidating recent advancements in the field, we aim to enhance the understanding of TIL spatial distribution and their immunomodulatory roles, thereby providing a comprehensive overview of TILs as pivotal components of the TME and their burgeoning potential in immunotherapeutic applications.

Overview of TIL dynamics in the TME

Within this TME network, lymphocytes are categorized based on their response mechanisms into adaptive and innate systems. Adaptive cells, such as T cells, B cells, and NKs, are defined by their antigen specificity and capacity to develop immunological memory. Innate immune cells like macrophages, neutrophils, and dendritic cells offer rapid, albeit non-specific defensive mechanisms.1 This section will distil recent research on the composition of TILs and unpack the intricacies of their infiltration and differentiation within the TME.

T cell populations: diversity and functionality

T cell differentiation within TILs varies depending on the cancer type and the influence of the TME. These cells can either suppress or promote tumorigenesis. T cell subsets are typically identified by their T cell receptor (TCR) and lineage markers CD4 and CD8. The αβ TCRs, categorized into CD4+ and CD8+ cells based on their major histocompatibility complex (MHC) class I and II restriction, form the dominant composition within the TIL population.5 Recent studies have revealed non-traditional T cell subsets, offering fresh insights due to their unique functions and impacts on the TME’s dynamics. For instance, significant proportions of T cells within lung tumors have been identified, highlighting the diversity and functional significance of T cell populations in cancer.6,7

TCR αβ+ T population

The TCR αβ+ population in the TME exhibits several distinct subsets that diverge from those found in peripheral blood due to tumorigenesis. CD8+ T cells and CD4+ T cells play significant roles in antitumor immunity, with their functionality influenced by the TME. Research has shown that CD4+ T cells are crucial in tumor immunity,8 with their activation or suppression altering cytokine levels and tumor growth dynamics. For instance, Friedman et al. demonstrated the importance of CD4+ T cells in tumor immunity by observing a reduction in interferon gamma (IFN-γ) levels upon blocking MHCII with human leukocyte antigen DR (HLA-DR) antibodies.8 Furthermore, Ben-Avi et al. found that while melanoma-infiltrating lymphocytes were enriched with CD8+ T cells, TILs from non-small cell lung cancer contained a higher proportion of CD4+ T cells.9 Additionally, ERBB2IP has been identified as a new target for tumor rejection, and reactivation of CD4+ T cells with an ERBB2IP mutation can lead to dramatic regression in liver and lung cancers.10

Apart from these, double-positive TCR αβ+ cells (DPTs) have been identified in lung cancer,11 breast cancer,12 kidney cancer,5,13 and melanoma,14 representing the functional characteristics of both CD4+ and CD8+ T cell populations.15 DPTs express activation markers such as HLA-DR, CD38, 4-1BB, CD137, and Ki-67, as well as inhibitory markers including programmed cell death protein 1 (PD-1), T-cell immunoglobulin and mucin-domain containing-3, and T cell immunoreceptor with Ig and ITIM domains (TIGIT). Additionally, DPTs produce conventional cytokines found in memory cells, such as interleukin (IL) 4, IL5, and IL13.12,14,16 Found in prostate cancer and lung cancer as well,17,18 double-negative T cells are implicated in immune regulation and suppression,17,19 playing pivotal roles in adoptive cellular therapies.20 Their regulatory-like phenotype and the distinctions from normal tissue counterparts offer insights into their function and potential alterations during tumor infiltration.5

Naïve T population

Mature naïve T cells, characterized by specific transcriptional factors, recirculate between secondary lymphoid organs,21 and are separated by the expression of transcriptional factors such as C-C chemokine receptor type 7, lymphoid enhancer-binding factor 1, and transcription factor 7.22 Mature naïve T cells, characterized by specific transcriptional factors, recirculate between secondary lymphoid organs.23,24

Th population

Derived from naïve CD4+ T cells, Helper T (Th) cells support the immune response by enhancing CD8+ T cell functions through cytokine secretion.1 Th cells are associated with favorable outcomes in various cancers and are classified based on their cytokine production profiles,25 liver cancer, biliary carcinoma,26,27 non-small cell lung cancer,28 nasopharyngeal carcinoma,29 triple-negative breast cancer,30 head and neck squamous carcinoma.31

Th cells are further classified by the production of lineage-specific cytokines. For instance, Th1 and Th2 cells dominantly secrete IFN-γ, IL1, and IL2, while Th17 cells are identified by the secretion of IL17.32 Studies in hepatocellular carcinoma patients undergoing neoadjuvant anti-PD-1 trials have highlighted the role of CXCL13+ Th cells in the response to immune checkpoint blockade within T cell-rich tumors.33 Furthermore, research has demonstrated that CD4+ Th cells facilitate the induction of anti-tumor responses by cytotoxic T lymphocytes (CTLs) through their interaction with classical type 1 dendritic cells ex vivo, indicating a positive correlation with prognosis.34

In addition to conventional Th cells, a population of T follicular helper cells has been identified in recent studies by their expression of the C-X-C chemokine receptor type 5 (CXCR5).5

Effector memory T (TEM) cells and tissue-resident memory (TRM) cells

TEM and TRM are independent prognostic indicators. High levels of TEM correlate with favorable cancer stages. Studies on colorectal cancer (CRC) have revealed that higher levels of infiltrating TEM are associated with low-grade tumor stage, limited invasion, and reduced lymph node metastasis. Furthermore, TRM have been linked to a favorable prognosis in various tumor types, with the CD103+ TRM subtype facilitating enhanced tumor homing through integrin expression on the cell surface. TEM have been identified as independent prognostic indicators of cancer and studies on CRC have revealed that higher levels of infiltrating TEM are associated with low-grade tumor stage, limited invasion, and reduced lymph node metastasis.35

CTLs

Predominately, the CD8+ TCRαβ+ T cell population, known as CTL, in the TME recognizes and destroys cancer cells displaying abnormal tumor antigens. Their presence often correlates with a positive prognosis in cancer patients. High levels of antitumor cytokines and cytotoxic molecules, including IFN-γ, tumor necrosis factor (TNF) α,36 granzyme B, and PRF1,22 are associated with CTL activity. On the other side, they exhibit high expression levels of inhibitory receptors, including PD-1, T cell immunoglobulin and mucin-domain containing-3, TIGIT, and lymphocyte-activation gene.5 CTLs provide long-term protection through memory cells, which have been shown to correlate with an overall positive prognosis in CRC and breast cancer.35,37 Recent single-cell sequencing (sc-seq) reveals the existence of CD4+ cytotoxic lymphocytes, expressing granzyme B,38 MX1,22 and TNF.37

Mucosal associated invariant T (MAIT) population

Despite the cell surface biomarkers of CD4 and CD8, MAIT are conventional conserved innate-like T subset. Despite their crucial role in regulating immunity and inflammation due to microbiota-derived signals,39 MAIT populations also show a significant impact on anti-tumor immunity. However, MAIT cells exhibit heterogeneity and dysfunctionality, with impaired infiltration into liver tumors. Interactions with CSF1R+PD-L1+ tumor-associated macrophages in the adjacent liver microenvironment play a key role in MAIT cell dysfunction.40 In addition to non-small cell lung cancer (NSCLC) patients, MAIT cells transition to an exhausted tumor-promoting phenotype, with circulating MAIT subsets serving as predictors for responsiveness to anti-PD-1 immunotherapy.41

TCR γδ+ T population

Apart from traditional T-cell subsets, TCR γδ+ T cells hardly express CD4 or CD8 cell surface biomarkers. γδ+ T cells induce anti-tumor effects specifically on cancer cells without affecting normal cells. Their genetic structure allows for easy therapeutic interventions. These cells can recognize a wide range of antigens, including lipids, phospho-antigens, and peptides, in both MHC-dependent and -independent manners, making them effective against tumors with low mutational loads and downregulated MHC.42 Furthermore, they have shown significant potential in immunotherapy, particularly in combination with immune checkpoint blockade, as they exhibit exhaustion in the TME and respond similarly to immune checkpoint inhibitors as conventional T cells.43

T cell exhaustion

Continuous antigen stimulation leads to a progressive decline in multiple functions of T cells, characterized by impaired proliferation and cytotoxicity, as well as upregulation of various inhibitory factors such as PD1, lymphocyte-activation gene 3 (LAG-3), TIGIT, T-cell immunoglobulin and mucin-domain containing-3, TIGIT and lymphocyte activation gene 3-like protein.44,45 This phenomenon, known as T cell exhaustion, has been observed in various cancers, progressing from a pre-exhaustion state to a dysfunctional cell state.

Exhausted T (Tex) population

The exhausted CD8+ T cell population comprises progenitor stem-like Tex cells and terminal Tex cells.46 The balance between these subsets is critical for an effective antitumor immune response.46,47 Progenitor stem-like exhausted T cells retain the ability for specific immune responses, self-renewal, and differentiation into TEX cells while exhibiting high expression of the transcription factor 1 and the chemotactic receptor CXCR5.46 TEX cells, predominantly express inhibitory cell surface receptors and transcription factors associated with cytolytic functions such as IFN-γ and granzyme B.5,37

Recent research by Liangtao Zheng et al. has inferred state-transition paths from a naïve to an exhausted state, elucidating the pan-cancer single-cell landscape.37 Exhausted T cells, particularly CD8+ T cells, account for a significant proportion of potentially tumor-reactive T cells and display high heterogeneity. The paths leading to exhaustion involve both TRM and TEM. In CD4+ T cells, potentially tumor-reactive T cells are predominantly follicular Th cells that exhibit dual functionality as Th1 cells.

Moreover, increased expression of CX3C chemokine receptor 1, a biomarker of T cell exhaustion and differentiation, is consistently accompanied by decreased expression of PD1, LAG-3, and TIGIT.48 CX3C chemokine receptor 1-negative cells have been shown to impede tumor proliferation and improve survival rates. Another crucial regulator is TOX, which plays a significant role in the formation of TEX and the expression of inhibitory receptors on T cells.5 T cells lacking TOX have lost their functions,49–51 suggesting that exhaustion may, to some extent, serve to protect T cells from tumor cell-induced injury or activation-induced cell death.5

Understanding the mechanisms of cell exhaustion may pave the way for novel directions in tumor immunity, such as enhancing immunotherapy by preserving the functionality of T cells before they become dysfunctional.

Regulatory T (Treg) population

Treg cells, also known as tumor-promoting CD4+CD25+FoxP3+ T cells, are the terminal CD4+ T population. They have garnered considerable attention due to their ability to inhibit the tumor-specific immune response of CTLs.52 Their actions include modulating NK cell homeostasis and indirectly supporting cancer cell survival via interactions with stromal cells.1

Tregs not only secrete immunosuppressive cytokines such as IL10, IL35, and transforming growth factor-β (TGF-β), but also exert inhibitory effects on the tumor immune environment by depleting nutrients, IL2, and inducing cytolysis.52 Persistently expressing CTLA4, Tregs competitively suppresses T cell activation by binding to CD80 and CD86 on antigen-presenting cells (APCs).53 Evidence suggests that tumor-infiltrating lymphocytes, particularly Foxp3+ Tregs in the tumor microenvironment, contribute to disease progression, invasion, and metastasis.54 Six studies on gastric cancer and CRC have found a correlation between high levels of Tregs and improved survival rates.55 However, technical challenges in quantifying cells adjacent to Tregs may have influenced these findings, as it is currently impossible to simultaneously evaluate.55

B cell population and their controversial roles

B cells contribute to tumor immunity through antigen presentation, antibody production, and cytokine secretion.56 Similar to dendritic cells (DCs), B cells possess antigen-presenting functions. Activated B cells (CD69+HLA-DR+CD27+CD21+) in non-small cell lung cancer have been found to present tumor-associated antigens to CD4+ T cells, highlighting the role of B cells as APCs. Additionally, plasma cells, a subtype of B cells responsible for antibody production, are present in tertiary lymphoid structures surrounding tumors.57 The coexistence of CD8+ T cells, CD20+ B cells, and plasma cells is significantly associated with a favorable prognosis. In high-grade ovarian cancer, this combination is correlated with a 10-year survival rate close to 65%.5

Contrarily, regulatory B cells (Bregs) are associated with a poor prognosis.58,59 Bregs inhibit tumor immune responses through the secretion of IL10 and TGF-β. IL10 affects the differentiation of DCs, leading to the suppression of T cell proliferation and promoting their differentiation into Tregs.59 Studies in ovarian cancer and liver cancer have shown that IL10 secreted by Bregs significantly suppresses tumor-specific T cell responses.58,60 However, in breast cancer and tongue squamous carcinoma models, Bregs inhibit the differentiation of CD4+ T cells into Tregs through TGF-β.61,62

Innate lymphoid cell (ILC): Emerging players in tumor immunity

ILCs, including NK cells and various subtypes, are recognized for their significant roles in maintaining immune homeostasis.

NK cells

NK cells, lacking antigen-specific B or T cell receptors due to the absence of recombination-activating genes,63,64 are positively associated with survival rates and negatively correlated with disease progression.5 They exhibit antitumor immunity by releasing granzyme and perforin and activating TNF-related apoptosis-inducing ligand.63,64 NK cells also produce chemotactic factors such as chemokine (C-C motif) ligand 5 (CCL5), XCL1, and XCL2 to activate conventional DCs, affecting tumor immunity.

ILCs

While NK cells are typically associated with positive survival outcomes, the roles of ILC subtypes in cancer are complex and varied. Some ILCs promote tumor defense, while others potentially aid in tumor escape. Although poorly understood, ILCs are believed to exhibit high plasticity and display contradictory roles in tumor immunity.65 Type I ILCs function similarly to NK cells by producing IFN-γ.66 Type II ILCs express IL33 receptors ST2 and CD127, and upon activation, they also express PD-1.67,68 Type III ILCs show contradictory manifestations in tumor immunity, promoting the formation of protective tumor-related tertiary lymphoid structures through TNF-α and IL22.69 However, a study on lung squamous carcinoma found that the transformation from type I and type II ILCs contributes to tumor immune escape, correlating with tumor growth and poor prognosis.5

Interestingly, a novel cell population known as innate-like T cells with high cytotoxic potential (ILTCKs) has been identified. ILTCKs are characterized by high expression of NK1.1, CD49a, and CD103.70 They exhibit unique tumor-elicited immune responses and share similarities with ILCs. While conventional T cells mainly recognize neoantigens, ILTCKs can respond to unmutated antigens in the absence of APCs.71

The multidimensional interactions of TILs within the TME

TME represents a multifaceted ecological system, encompassing not only malignant cells but also an array of immune cells, vasculature, and components of the nervous system. This intricate milieu acts as a battleground where the immune system and cancer cells dynamically interact. Within this framework, the composition of the TME and the presence of immune biomarkers hold significant prognostic value for tumor evaluation and treatment outcome.72,73 The heterogeneity and functionality of TILs are particularly varied across different cancer types. For instance, in liver cancer, TILs present distinct clonality and exhaustion patterns, pivotal for understanding their role in tumor progression.45

Cellular interaction in the TME

The immune milieu within the TME profoundly influences the functionality of TILs. During the incipient stages of oncogenesis, cancer cells may evade innate immunity, leading to the development of functionally compromised T and B cells.74,75 Additionally, nutrient scarcity within the TME compels TILs to assume metabolic adaptations conducive to tolerogenic phenotypes, thereby diminishing their antitumor efficacy.76 Furthermore, Myeloid-derived suppressor cells exert suppressive actions on T cell proliferation and the activation of NK and T cells, while simultaneously enhancing regulatory Treg functions.77 In breast cancer, tumor-associated fibroblasts contribute to intratumoral vascularization and the recruitment of immune cells.78

A pivotal study on the Tumor Immune Microenvironment in primary liver cancer identified five distinct subtypes through single-cell RNA sequencing, delineating immune activation, suppression, exclusion, and residence phenotypes, each mediated by different cellular clusters and genomic characteristics.79

Chemokines, cytokine, and TIL recruitment

The cytokine milieu within the TME provides indirect insight into the interactions between the TME and TILs. In hepatocellular carcinoma by Chew et al., tumor escape is predominantly facilitated by immunosuppressive chemokines released by T cells within the TME, while therapy resistance correlates with immune neglect in regions lacking T-cell infiltration.80–82

Cytokines are instrumental in modulating immune checkpoints, including CTLA-4 on T cells, which attenuates T cell activation, and PD-1 on tumor cells, promoting immune suppression.72 Conversely, PD-1 expressed by tumor cells interferes with immune-suppressive pathways.83 Teng et al. introduced a classification of the TME into four types based on the presence of TILs and PD-L1 expression,84 aiding in predicting the tumor’s response to therapies targeting PD-1/PD-L1 pathways and serving as a valuable prognostic tool.

The TME can both hinder and support the function of TILs. Factors such as local immunosuppression, the presence of Tregs, and the secretion of various cytokines and growth factors can dramatically alter the activity of TILs within tumors. Chemokines such as CXCL10 and CCL5 play a pivotal role in recruiting TILs to the tumor site, enhancing the infiltration of T cells and NK cells, thereby increasing tumor cell death.

Clinical values of TILs

TILs have fundamentally transformed the approach to clinical prognosis and immunotherapy for hematologic cancers. However, the diversity of solid tumors necessitates utilizing TILs with broad specificity. Recent advancements in understanding the TME, immune exhaustion, and immune checkpoints have paved the way for applying TIL clinical regimens.

Prognostic value

The composition and number of TILs are critical to tumor prognosis.

T cell population

A systematic review and meta-analysis focusing on laryngeal squamous cell carcinoma (LSCC) revealed that TILs, particularly stromal TILs, play a favorable prognostic role for overall survival (OS) and disease-free survival (DFS). High levels of CD8+ TILs were associated with prolonged OS and DFS, reinforcing the importance of TILs as biomarkers in cancer prognosis.85 Daniele Fanale et al. demonstrated the significant prognostic value of TILs in cancers like ovarian cancer.86 High densities of TILs, particularly CD8+ cytotoxic T cells, are often associated with better survival outcomes. This comprehensive analysis, encompassing 11 studies with 1,398 patients, confirmed the strong prognostic value of TILs for OS and DFS in LSCC. The findings highlight the significance of immune infiltrates in the TME of head and neck squamous cell carcinoma and suggest their potential as standardized and validated biomarkers in routine pathology reports.85 The identification and validation of biomarkers that can accurately predict patient response to immune checkpoint blockade therapies are crucial for developing personalized treatment strategies and potentially increasing the success rate of these therapies. The meta-analysis underscores the need to standardize TIL assessment in clinical pathology, especially for patients with LSCC. Assessing TILs using hematoxylin-eosin-stained tumor sections or immunohistochemistry could provide valuable insights into patient prognosis and guide treatment strategies.85

B cell population

While immunotherapy research typically focuses on T cells, mounting evidence shows that tumor-infiltrating B cells and plasma cells, collectively referred to as tumor-infiltrating B lymphocytes, play a crucial and synergistic role in tumor control. They have significant predictive and prognostic implications in many cancers, despite the conventional emphasis on T cells in immunotherapy. The presence of T cells and myeloid cells in the TME is a prerequisite for infiltrating B cells, whose dysfunction with CXCL13 activates the recruitment of B cells and plasma cells in the TLS.87 TIL-Bs promote anti-tumor immune responses through their unique antigen presentation mechanisms, contribute to the formation of immunologically active tumor microenvironments, and possess the potential to counter immune editing and address tumor heterogeneity. This offers hope for developing new treatment approaches and more effective immunotherapy options for cancer patients.

Therapeutic targeting

For solid tumors, TILs play critical roles in innate and adaptive antitumor immunity. Studies have shown that combination therapy can increase the infiltration of lymphocytes, including CD8+ T cells, into the TME compared to monotherapy with anti-PD-L1 or β-lap, highlighting the impact of therapeutic interventions on TILs.88,89 Traditional chemotherapy has evolved into more targeted approaches focusing on specific cells within the TME. Immune checkpoint blockade therapies, like those targeting CTLA-4 and PD-1, have shown significant clinical benefits but are effective in only a subset of patients.72 The identification of relevant biomarkers is crucial to determine patient responsiveness to these therapies.

The advent of immune checkpoint blockade therapy marked a significant shift in cancer treatment by targeting specific immune cells within the TME. By blocking receptor-ligand interactions like CTLA-4 and PD-1, these therapies aim to enhance T-cell activation and function. Despite their potential, a majority of patients remain unresponsive, highlighting the need for precise biomarkers to predict treatment efficacy.1

Therapeutic implications and future directions

With the technical development of sc-seq and spatially resolved transcriptomics, numerous studies have demonstrated more potential for TILs and indicated future directions. In NSCLC patients undergoing neoadjuvant PD-1 blockade combined with chemotherapy, sc-seq identified distinct NSCLC tumor microenvironment transcriptomes correlated with therapy response, offering novel biomarkers and potential strategies to overcome immunotherapy resistance, despite limitations of small patient sample size and combination therapy.90 Another study on NSCLC identified mutation-associated neoantigens (MANA)-specific T cell clones in neoadjuvant anti-PD-1-treated non-small cell lung cancers, revealing unique transcriptional programs. MANA-specific CD8 T cells exhibit features of tissue-resident memory cells but display incomplete cytolytic activation, providing insights into overcoming resistance to PD-1 blockade.50

Despite significant developments in TIL treatment, it has been observed that TIL activity and numbers decrease, and their antitumor functions are limited or even altered during the infiltration process. Enhancing lymphocyte infiltration, identifying therapeutic targets, and selecting TILs could provide more possibilities for antitumor immunity. There are 43 ongoing clinical trials of TILs and related drugs (Table 1), which will further implicate the clinical application of TILs in the future. Future research should focus on unraveling the complex interactions within the TME and harnessing this knowledge for therapeutic benefit.

Table 1

Comprehensive overview of clinical trials on tumor infiltrating lymphocyte therapy for advanced solid tumors

NCT numberStudy titleStudy statusInterventions
NCT05035407T cell receptor (TCR) gene therapy targeting Kita-kyushu lung cancer antigen 1 (KK-LC-1) for gastric, breast, cervical, lung and other KK-LC-1 positive epithelial cancersRecruitingDrug: interleukin 2 (IL-2) (aldesleukin)|
Drug: cyclophosphamide|
Biological: KK-LC-1 TCR |
Drug: fludarabine
NCT05902520Adoptive cell therapy using cancer specific CD8+ tumor infiltrating lymphocyte (TIL) in adult patients with solid tumorsRecruitingBiological: double positive (DP) CD8 TIL |
Biological: DP CD8 TIL after programmed death-1 (PD-1) knockdown |
Biological: low dose IL-2
NCT05831033Safety and efficacy of an autologous TIL therapy in patients with advanced solid tumorsNot_yet_recruitingBiological: BEN101
NCT04842812Engineered TIL/ cheric antigen receptor (CAR)-TIL to treat advanced solid tumorsRecruitingBiological: TIL and CAR-TIL targeting human epidermal growth factor receptor 2 (HER2), Mesothelin, prostate stem cell antigen (PSCA), mucin 1, Lewis Y antigen, glypican 3 (GPC3), AXL receptor tyrosine kinase (AXL), epidermal growth factor receptor (EGFR), claudin 18.2 and claudin 6 (Claudin18.2/6), receptor tyrosine kinase-like orphan receptor 1 (ROR1), Ganglioside GD1 (GD1), or B7 homolog 3 protein (B7-H3)
NCT04967833Study on TIL for the treatment of advanced solid tumorsRecruitingBiological: TIL
NCT06077903GT101 injection in the treatment of metastatic/recurrent advanced solid tumorsRecruitingBiological: GT101
NCT05087745A clinical study on TIL for the treatment of advanced solid tumorsRecruitingBiological: tumor infiltrating lymphocytes
NCT03449108Ln-145 or ln-145-s1 in treating patients with relapsed or refractory ovarian cancer, triple negative breast cancer (TNBC), anaplastic thyroid cancer, osteosarcoma, or other bone and soft tissue sarcomasActiveBiological: aldesleukin|
Biological: autologous tumor infiltrating lymphocytes LN-145|
Biological: autologous tumor infiltrating lymphocytes LN-145-S1|
Drug: cyclophosphamide|
Drug: fludarabine|
Biological: Ipilimumab|
Biological: nivolumab|
Other: quality-of-life assessment|
Other: questionnaire administration
NCT06094426Autologous tumor infiltrating lymphocyte injection for the treatment of advanced solid tumorsRecruitingBiological: GT316
NCT03935893Adoptive transfer of tumor infiltrating lymphocytes for advanced solid cancersRecruitingBiological: TIL|
Drug: fludarabine + cyclophosphamide combination
NCT05397093ITIL-306 in advanced solid tumorsActiveBiological: ITIL-306
NCT06144671GT201 Injection for the treatment of advanced solid tumorsRecruitingDrug: GT201
NCT05499715A phase I clinical study to evaluate the safety, the tolerability, the pharmacokinetic characteristics and the efficacy of scTIL injection (genetically modified tumor infiltrating lymphocytes) in the treatment of advanced malignant solid tumorsNot_yet_recruitingBiological: scTIL injection
NCT05868915HV-101 for patients with advanced solid tumorsRecruitingBiological: HV-101
NCT05417750A phase I study on autologous tumor infiltrating lymphocytes injection (GC101 TIL) for the treatment of advanced malignant solid tumorsRecruitingDrug: TIL therapy
NCT05539768Study on the safety and efficacy of autogenous tumor infiltrates lymphocytes for the treatment of advanced solid tumorNot_yet_recruitingBiological: HS-IT101
NCT03610490Autologous TIL MDA-TIL in treating patients with recurrent or refractory ovarian cancer, colorectal cancer, or pancreatic ductal adenocarcinomaActive_not_recruitingBiological: autologous TIL MDA-TIL|
Drug: cyclophosphamide|
Drug: fludarabine|
Biological: IL-2|
Other: quality-of-life assessment
NCT05573035A study to investigate LYL845 in adults with solid tumorsRecruitingBiological: LYL845
NCT03991741Adoptive cell transfer of autologous tumor infiltrating lymphocytes and high-dose IL 2 in select solid tumorsRecruitingBiological: autologous tumor infiltrating lymphocytes|
Biological: high-dose interleukin 2
NCT05971576A clinical study on TILs for the treatment of advanced solid tumorsNot_yet_recruitingBiological: LM103
NCT05141474Assessment of the safety and tolerability of ex vivo next-generation neoantigen-selected TIL therapy in advanced epithelial tumors and immune checkpoint blockade (ICB) resistant solid tumorsRecruitingBiological: NEXTGEN-TIL|
Drug: non-myeloablative lymphodepletion (NMA-LD) regimen|
Drug: interleukin-2
NCT06060613Safety and efficacy of OBX-115 in advanced solid tumorsRecruitingBiological: OBX-115
NCT02650986Gene-modified T cells with or without decitabine in treating patients with advanced malignancies expressing New York Esophageal Squamous Cell Carcinoma 1 (NY-ESO-1)Active_not_recruitingDrug: cyclophosphamide|
Drug: decitabine|
Other: laboratory biomarker analysis|
Procedure: leukapheresis|
Biological: TGFBDNRII-transduced autologous tumor infiltrating lymphocytes
NCT04571892A clinical study to observe the safety and efficacy of ScTIL210 in the treatment of malignant solid tumorsRecruitingBiological: super circulating tumor infiltrating lymphocytes (ScTIL)
NCT03658785Immunotherapy for the treatment of advanced solid tumorRecruitingBiological: TIL|
Drug: aldesleukin|
Drug: cyclophosphamide|
Drug: fludarabine
NCT06088472TIL injection for the treatment of metastatic or recurrent solid tumorsRecruitingBiological: TIL injection
NCT05971589A clinical study on LM103 injection for the treatment of advanced solid tumorsNot_yet_recruitingBiological: LM103
NCT05730361Phase Ib clinical study on the safety, the tolerability, the pharmacokinetics and the efficacy of ScTIL injection (gene modified tumor infiltrating lymphocytes) alone and in combination with B lymphocyte adjuvant in the treatment of digestive system malignant solid tumorsNot_yet_recruitingBiological: ScTIL injection|
Biological: ScTIL injection and B lymphocytes adjuvant
NCT05724732Exploratory clinical study of autologous tumor-infiltrating lymphocyte injection (GT201) for advanced gynecologic tumorsRecruitingBiological: GT201
NCT05576077A study of tbio-4101 (TIL) and pembrolizumab in patients with advanced solid tumorsRecruitingBiological: TBio-4101|
Drug: pembrolizumab
NCT06107894TIL therapy for patients with advanced solid tumorsNot_yet_recruitingBiological: tumor-infiltrating lymphocytes|
Drug: IL-2
NCT05649618TIL cells for the treatment of the advanced solid tumors patientsNot_yet_recruitingBiological: tumor infiltrating lymphocytes|
Drug: fludarabine|
Drug: cyclophosphamide capsules|drug: IL-2
NCT06145802Clinical study of autologous tumor infiltrating lymphocyte injection (GT316) in the treatment of advanced solid tumors (gynecological tumors)RecruitingBiological: GT316
NCT04114136Anti-programmed death-1 monoclonal antibody (anti-PD-1 mAb) plus metabolic modulator in solid tumor malignanciesRecruitingDrug: nivolumab or pembrolizumab (dependent upon approved indication)|
Drug: metformin|
Drug: rosiglitazone
NCT05430373GT101 injection for the treatment of metastatic or recurrent solid tumorsRecruitingBiological: GT101
NCT06047977Tumor infiltrating lymphocyte therapy for pediatric high risk solid tumorsNot_yet_recruitingBiological: tumor infiltrating lymphocytes, fludarabine, cyclophosphamide, interleukin-2
NCT05366478A clinical study of LM103 injection in the treatment of advanced solid tumorsRecruitingDrug: autologous TILs
NCT03645928Study of autologous tumor infiltrating lymphocytes in patients with solid tumorsRecruitingBiological: lifileucel|biological: ln-145|
Drug: pembrolizumab|
Biological: ln-145-s1|
Drug: ipilimumab|
Drug: nivolumab
NCT05941936A clinical study on LM103 for the treatment of advanced solid tumorsRecruitingBiological: LM103
NCT04643574NeoTIL in advanced solid tumorsActive_not_recruitingBiological: NeoTIL|
Drug: cyclophosphamide|
Drug: fludarabine|
Drug: interleukin-2|Radiation: radiotherapy
NCT06237881A phase 1/2 Study of KSQ-001EX, autologous tumor infiltrating lymphocytes engineered to inactivate the suppressor of cytokine signaling 1 (SOCS1) gene, in patients with select advanced solid tumorsNot_yet_recruitingDrug: KSQ-001EX|
Drug: IL-2|
Drug: cyclophosphamide|
Drug: fludarabine
NCT05729399Clinical study of autologous tumor-infiltrating lymphocyte injection (GT201) for advanced solid tumorsRecruitingBiological: GT201

Conclusions

TILs, especially T cells, are integral to orchestrating effective immune responses against tumors. Advances in technologies like sc-seq and spatiomics have enhanced our understanding of TILs. This review emphasized the pivotal role of TILs, discusses their potential as therapeutic targets and prognostic markers, and underscores the need for further research to unravel the complex interactions within the TME for effective cancer immunotherapy.

Declarations

Acknowledgement

None.

Funding

None.

Conflict of interest

The authors have no conflict of interest related to this publication.

Authors’ contributions

Study concept and design (XZ, SJ), acquisition, analysis and interpretation of data (LP, ZS), drafting of the manuscript (ZS), critical revision of the manuscript for important intellectual content (XZ, SJ), administrative, technical, or material support and study supervision (SJ). All authors have made a significant contribution to this study and have approved the final manuscript.

References

  1. Anderson NM, Simon MC. The tumor microenvironment. Curr Biol 2020;30(16):R921-R925 View Article PubMed/NCBI
  2. Gorczynski RM. Tumor Microenvironment: The Role of Interleukins — Part A. Cham: Springer; 2020, 47-58 View Article
  3. Rosenberg SA, Spiess P, Lafreniere R. A new approach to the adoptive immunotherapy of cancer with tumor-infiltrating lymphocytes. Science 1986;233(4770):1318-1321 View Article PubMed/NCBI
  4. Dougan M, Dranoff G, Dougan SK. Cancer Immunotherapy: Beyond Checkpoint Blockade. Annu Rev Cancer Biol 2019;3:55-75 View Article PubMed/NCBI
  5. Paijens ST, Vledder A, de Bruyn M, Nijman HW. Tumor-infiltrating lymphocytes in the immunotherapy era. Cell Mol Immunol 2021;18(4):842-859 View Article PubMed/NCBI
  6. Kataki A, Scheid P, Piet M, Marie B, Martinet N, Martinet Y, et al. Tumor infiltrating lymphocytes and macrophages have a potential dual role in lung cancer by supporting both host-defense and tumor progression. J Lab Clin Med 2002;140(5):320-328 View Article PubMed/NCBI
  7. Lavin Y, Kobayashi S, Leader A, Amir ED, Elefant N, Bigenwald C, et al. Innate Immune Landscape in Early Lung Adenocarcinoma by Paired Single-Cell Analyses. Cell 2017;169(4):750-765.e17 View Article PubMed/NCBI
  8. Friedman KM, Prieto PA, Devillier LE, Gross CA, Yang JC, Wunderlich JR, et al. Tumor-specific CD4+ melanoma tumor-infiltrating lymphocytes. J Immunother 2012;35(5):400-408 View Article PubMed/NCBI
  9. Ben-Avi R, Farhi R, Ben-Nun A, Gorodner M, Greenberg E, Markel G, et al. Establishment of adoptive cell therapy with tumor infiltrating lymphocytes for non-small cell lung cancer patients. Cancer Immunol Immunother 2018;67(8):1221-1230 View Article PubMed/NCBI
  10. Tran E, Turcotte S, Gros A, Robbins PF, Lu YC, Dudley ME, et al. Cancer immunotherapy based on mutation-specific CD4+ T cells in a patient with epithelial cancer. Science 2014;344(6184):641-645 View Article PubMed/NCBI
  11. Boulle G, Velut Y, Mansuet-Lupo A, Gibault L, Blons H, Fournel L, et al. Chemoradiotherapy efficacy is predicted by intra-tumour CD8+/FoxP3+ double positive T cell density in locally advanced N2 non-small-cell lung carcinoma. Eur J Cancer 2020;135:221-229 View Article PubMed/NCBI
  12. Desfrançois J, Derré L, Corvaisier M, Le Mével B, Catros V, Jotereau F, et al. Increased frequency of nonconventional double positive CD4CD8 alphabeta T cells in human breast pleural effusions. Int J Cancer 2009;125(2):374-380 View Article PubMed/NCBI
  13. Menard LC, Fischer P, Kakrecha B, Linsley PS, Wambre E, Liu MC, et al. Renal Cell Carcinoma (RCC) Tumors Display Large Expansion of Double Positive (DP) CD4+CD8+ T Cells With Expression of Exhaustion Markers. Front Immunol 2018;9:2728 View Article PubMed/NCBI
  14. Desfrançois J, Moreau-Aubry A, Vignard V, Godet Y, Khammari A, Dréno B, et al. Double positive CD4CD8 alphabeta T cells: a new tumor-reactive population in human melanomas. PLoS One 2010;5(1):e8437 View Article PubMed/NCBI
  15. Parrot T, Oger R, Allard M, Desfrançois J, Raingeard de la Blétière D, Coutolleau A, et al. Transcriptomic features of tumour-infiltrating CD4(low)CD8(high) double positive αβ T cells in melanoma. Sci Rep 2020;10(1):5900 View Article PubMed/NCBI
  16. Bohner P, Chevalier MF, Cesson V, Rodrigues-Dias SC, Dartiguenave F, Burruni R, et al. Double Positive CD4+CD8+ T Cells Are Enriched in Urological Cancers and Favor T Helper-2 Polarization. Front Immunol 2019;10:622 View Article PubMed/NCBI
  17. Lu Y, Hu P, Zhou H, Yang Z, Sun YU, Hoffman RM, et al. Double-negative T Cells Inhibit Proliferation and Invasion of Human Pancreatic Cancer Cells in Co-culture. Anticancer Res 2019;39(11):5911-5918 View Article PubMed/NCBI
  18. Stankovic B, Bjørhovde HAK, Skarshaug R, Aamodt H, Frafjord A, Müller E, et al. Immune Cell Composition in Human Non-small Cell Lung Cancer. Front Immunol 2018;9:3101 View Article PubMed/NCBI
  19. Strober S, Cheng L, Zeng D, Palathumpat R, Dejbakhsh-Jones S, Huie P, et al. Double negative (CD4-CD8- alpha beta+) T cells which promote tolerance induction and regulate autoimmunity. Immunol Rev 1996;149:217-230 View Article PubMed/NCBI
  20. Lee J, Minden MD, Chen WC, Streck E, Chen B, Kang H, et al. Allogeneic Human Double Negative T Cells as a Novel Immunotherapy for Acute Myeloid Leukemia and Its Underlying Mechanisms. Clin Cancer Res 2018;24(2):370-382 View Article PubMed/NCBI
  21. Brummelman J, Pilipow K, Lugli E. The Single-Cell Phenotypic Identity of Human CD8(+) and CD4(+) T Cells. Int Rev Cell Mol Biol 2018;341:63-124 View Article PubMed/NCBI
  22. Schalck A, Sakellariou-Thompson D, Forget MA, Sei E, Hughes TG, Reuben A, et al. Single-Cell Sequencing Reveals Trajectory of Tumor-Infiltrating Lymphocyte States in Pancreatic Cancer. Cancer Discov 2022;12(10):2330-2349 View Article PubMed/NCBI
  23. Yang Y, Li G, Lu Z, Liu Y, Kong J, Liu J. Progression of Prothrombin Induced by Vitamin K Absence-II in Hepatocellular Carcinoma. Front. Oncol 2021;11:726213 View Article
  24. Raeber ME, Zurbuchen Y, Impellizzieri D, Boyman O. The role of cytokines in T-cell memory in health and disease. Immunol Rev 2018;283(1):176-193 View Article PubMed/NCBI
  25. Yagi T, Baba Y, Ishimoto T, Iwatsuki M, Miyamoto Y, Yoshida N, et al. PD-L1 Expression, Tumor-infiltrating Lymphocytes, and Clinical Outcome in Patients With Surgically Resected Esophageal Cancer. Ann Surg 2019;269(3):471-478 View Article PubMed/NCBI
  26. Tanaka R, Kimura K, Eguchi S, Tauchi J, Shibutani M, Shinkawa H, et al. Preoperative Neutrophil-to-lymphocyte Ratio Predicts Tumor-infiltrating CD8(+) T Cells in Biliary Tract Cancer. Anticancer Res 2020;40(5):2881-2887 View Article PubMed/NCBI
  27. Cai XY, Gao Q, Qiu SJ, Ye SL, Wu ZQ, Fan J, et al. Dendritic cell infiltration and prognosis of human hepatocellular carcinoma. J Cancer Res Clin Oncol 2006;132(5):293-301 View Article PubMed/NCBI
  28. Yoneda K, Kuwata T, Kanayama M, Mori M, Kawanami T, Yatera K, et al. Alteration in tumoural PD-L1 expression and stromal CD8-positive tumour-infiltrating lymphocytes after concurrent chemo-radiotherapy for non-small cell lung cancer. Br J Cancer 2019;121(6):490-496 View Article PubMed/NCBI
  29. Ono T, Azuma K, Kawahara A, Sasada T, Matsuo N, Kakuma T, et al. Prognostic stratification of patients with nasopharyngeal carcinoma based on tumor immune microenvironment. Head Neck 2018;40(9):2007-2019 View Article PubMed/NCBI
  30. Hollern DP, Xu N, Thennavan A, Glodowski C, Garcia-Recio S, Mott KR, et al. B Cells and T Follicular Helper Cells Mediate Response to Checkpoint Inhibitors in High Mutation Burden Mouse Models of Breast Cancer. Cell 2019;179(5):1191-1206.e1121 View Article
  31. Nguyen N, Bellile E, Thomas D, McHugh J, Rozek L, Virani S, et al. Tumor infiltrating lymphocytes and survival in patients with head and neck squamous cell carcinoma. Head Neck 2016;38(7):1074-1084 View Article PubMed/NCBI
  32. Zhu J. T Helper Cell Differentiation, Heterogeneity, and Plasticity. Cold Spring Harb Perspect Biol 2018;10(10):a030338 View Article PubMed/NCBI
  33. Magen A, Hamon P, Fiaschi N, Soong BY, Park MD, Mattiuz R, et al. Intratumoral dendritic cell-CD4(+) T helper cell niches enable CD8(+) T cell differentiation following PD-1 blockade in hepatocellular carcinoma. Nat Med 2023;29(6):1389-1399 View Article PubMed/NCBI
  34. Lei X, Khatri I, de Wit T, de Rink I, Nieuwland M, Kerkhoven R, et al. CD4(+) helper T cells endow cDC1 with cancer-impeding functions in the human tumor micro-environment. Nat Commun 2023;14(1):217 View Article PubMed/NCBI
  35. Pagès F, Berger A, Camus M, Sanchez-Cabo F, Costes A, Molidor R, et al. Effector memory T cells, early metastasis, and survival in colorectal cancer. N Engl J Med 2005;353(25):2654-2666 View Article PubMed/NCBI
  36. Beumer-Chuwonpad A, Taggenbrock RLRE, Ngo TA, van Gisbergen KPJM. The Potential of Tissue-Resident Memory T Cells for Adoptive Immunotherapy against Cancer. Cells 2021;10(9):2234 View Article PubMed/NCBI
  37. Zheng L, Qin S, Si W, Wang A, Xing B, Gao R, et al. Pan-cancer single-cell landscape of tumor-infiltrating T cells. Science 2021;374(6574):abe6474 View Article PubMed/NCBI
  38. Ahmadvand S, Faghih Z, Montazer M, Safaei A, Mokhtari M, Jafari P, et al. Importance of CD45RO+ tumor-infiltrating lymphocytes in post-operative survival of breast cancer patients. Cell Oncol (Dordr) 2019;42(3):343-356 View Article PubMed/NCBI
  39. Legoux F, Salou M, Lantz O. MAIT Cell Development and Functions: the Microbial Connection. Immunity 2020;53(4):710-723 View Article PubMed/NCBI
  40. Ruf B, Bruhns M, Babaei S, Kedei N, Ma L, Revsine M, et al. Tumor-associated macrophages trigger MAIT cell dysfunction at the HCC invasive margin. Cell 2023;186(17):3686-3705.e32 View Article PubMed/NCBI
  41. Shi L, Lu J, Zhong D, Song M, Liu J, You W, et al. Clinicopathological and predictive value of MAIT cells in non-small cell lung cancer for immunotherapy. J Immunother Cancer 2023;11(1):e005902 View Article PubMed/NCBI
  42. Deng J, Yin H. Gamma delta (γδ) T cells in cancer immunotherapy; where it comes from, where it will go?. Eur J Pharmacol 2022;919:174803 View Article PubMed/NCBI
  43. Gao Z, Bai Y, Lin A, Jiang A, Zhou C, Cheng Q, et al. Gamma delta T-cell-based immune checkpoint therapy: attractive candidate for antitumor treatment. Mol Cancer 2023;22(1):31 View Article PubMed/NCBI
  44. Egelston CA, Avalos C, Tu TY, Simons DL, Jimenez G, Jung JY, et al. Human breast tumor-infiltrating CD8(+) T cells retain polyfunctionality despite PD-1 expression. Nat Commun 2018;9(1):4297 View Article PubMed/NCBI
  45. Zheng C, Zheng L, Yoo JK, Guo H, Zhang Y, Guo X, et al. Landscape of Infiltrating T Cells in Liver Cancer Revealed by Single-Cell Sequencing. Cell 2017;169(7):1342-1356.e16 View Article PubMed/NCBI
  46. Miller BC, Sen DR, Al Abosy R, Bi K, Virkud YV, LaFleur MW, et al. Subsets of exhausted CD8(+) T cells differentially mediate tumor control and respond to checkpoint blockade. Nat Immunol 2019;20(3):326-336 View Article PubMed/NCBI
  47. Siddiqui I, Schaeuble K, Chennupati V, Fuertes Marraco SA, Calderon-Copete S, Pais Ferreira D, et al. Intratumoral Tcf1(+)PD-1(+)CD8(+) T Cells with Stem-like Properties Promote Tumor Control in Response to Vaccination and Checkpoint Blockade Immunotherapy. Immunity 2019;50(1):195-211.e10 View Article PubMed/NCBI
  48. Yamauchi T, Hoki T, Oba T, Saito H, Attwood K, Sabel MS, et al. CX3CR1-CD8+ T cells are critical in antitumor efficacy but functionally suppressed in the tumor microenvironment. JCI Insight 2020;5(8):133920 View Article PubMed/NCBI
  49. Mann TH, Kaech SM. Tick-TOX, it’s time for T cell exhaustion. Nat Immunol 2019;20(9):1092-1094 View Article PubMed/NCBI
  50. Khan O, Giles JR, McDonald S, Manne S, Ngiow SF, Patel KP, et al. TOX transcriptionally and epigenetically programs CD8(+) T cell exhaustion. Nature 2019;571(7764):211-218 View Article PubMed/NCBI
  51. Scott AC, Dündar F, Zumbo P, Chandran SS, Klebanoff CA, Shakiba M, et al. TOX is a critical regulator of tumour-specific T cell differentiation. Nature 2019;571(7764):270-274 View Article PubMed/NCBI
  52. Kumar P, Bhattacharya P, Prabhakar BS. A comprehensive review on the role of co-signaling receptors and Treg homeostasis in autoimmunity and tumor immunity. J Autoimmun 2018;95:77-99 View Article PubMed/NCBI
  53. Qureshi OS, Zheng Y, Nakamura K, Attridge K, Manzotti C, Schmidt EM, et al. Trans-endocytosis of CD80 and CD86: a molecular basis for the cell-extrinsic function of CTLA-4. Science 2011;332(6029):600-603 View Article PubMed/NCBI
  54. Zeng R, Lyu Y, Zhang G, Shou T, Wang K, Niu H, et al. Positive effect of RORγt on the prognosis of thyroid papillary carcinoma patients combined with Hashimoto’s thyroiditis. Am J Transl Res 2018;10(10):3011-3024 PubMed/NCBI
  55. Fridman WH, Zitvogel L, Sautès-Fridman C, Kroemer G. The immune contexture in cancer prognosis and treatment. Nat Rev Clin Oncol 2017;14(12):717-734 View Article PubMed/NCBI
  56. Hu Q, Hong Y, Qi P, Lu G, Mai X, Xu S, et al. Atlas of breast cancer infiltrated B-lymphocytes revealed by paired single-cell RNA-sequencing and antigen receptor profiling. Nat Commun 2021;12(1):2186 View Article PubMed/NCBI
  57. Kroeger DR, Milne K, Nelson BH. Tumor-Infiltrating Plasma Cells Are Associated with Tertiary Lymphoid Structures, Cytolytic T-Cell Responses, and Superior Prognosis in Ovarian Cancer. Clin Cancer Res 2016;22(12):3005-3015 View Article PubMed/NCBI
  58. Cai X, Zhang L, Wei W. Regulatory B cells in inflammatory diseases and tumor. Int Immunopharmacol 2019;67:281-286 View Article PubMed/NCBI
  59. Madan R, Demircik F, Surianarayanan S, Allen JL, Divanovic S, Trompette A, et al. Nonredundant roles for B cell-derived IL-10 in immune counter-regulation. J Immunol 2009;183(4):2312-2320 View Article PubMed/NCBI
  60. Xiao X, Lao XM, Chen MM, Liu RX, Wei Y, Ouyang FZ, et al. PD-1hi Identifies a Novel Regulatory B-cell Population in Human Hepatoma That Promotes Disease Progression. Cancer Discov 2016;6(5):546-559 View Article PubMed/NCBI
  61. Olkhanud PB, Damdinsuren B, Bodogai M, Gress RE, Sen R, Wejksza K, et al. Tumor-evoked regulatory B cells promote breast cancer metastasis by converting resting CD4+ T cells to T-regulatory cells. Cancer Res 2011;71(10):3505-3515 View Article PubMed/NCBI
  62. Zhou X, Su YX, Lao XM, Liang YJ, Liao GQ. CD19(+)IL-10(+) regulatory B cells affect survival of tongue squamous cell carcinoma patients and induce resting CD4(+) T cells to CD4(+)Foxp3(+) regulatory T cells. Oral Oncol 2016;53:27-35 View Article PubMed/NCBI
  63. Du Y, Wei Y. Therapeutic Potential of Natural Killer Cells in Gastric Cancer. Front Immunol 2018;9:3095 View Article PubMed/NCBI
  64. Hoogstad-van Evert JS, Bekkers R, Ottevanger N, Jansen JH, Massuger L, Dolstra H. Harnessing natural killer cells for the treatment of ovarian cancer. Gynecol Oncol 2020;157(3):810-816 View Article PubMed/NCBI
  65. Vivier E, Artis D, Colonna M, Diefenbach A, Di Santo JP, Eberl G, et al. Innate Lymphoid Cells: 10 Years On. Cell 2018;174(5):1054-1066 View Article PubMed/NCBI
  66. Cortez VS, Colonna M. Diversity and function of group 1 innate lymphoid cells. Immunol Lett 2016;179:19-24 View Article PubMed/NCBI
  67. Moral JA, Leung J, Rojas LA, Ruan J, Zhao J, Sethna Z, et al. ILC2s amplify PD-1 blockade by activating tissue-specific cancer immunity. Nature 2020;579(7797):130-135 View Article PubMed/NCBI
  68. Demaria O, Vivier E. Immuno-Oncology beyond TILs: Unleashing TILCs. Cancer Cell 2020;37(4):428-430 View Article PubMed/NCBI
  69. Carrega P, Loiacono F, Di Carlo E, Scaramuccia A, Mora M, Conte R, et al. NCR(+)ILC3 concentrate in human lung cancer and associate with intratumoral lymphoid structures. Nat Commun 2015;6:8280 View Article PubMed/NCBI
  70. Dadi S, Chhangawala S, Whitlock BM, Franklin RA, Luo CT, Oh SA, et al. Cancer Immunosurveillance by Tissue-Resident Innate Lymphoid Cells and Innate-like T Cells. Cell 2016;164(3):365-377 View Article PubMed/NCBI
  71. Chou C, Zhang X, Krishna C, Nixon BG, Dadi S, Capistrano KJ, et al. Programme of self-reactive innate-like T cell-mediated cancer immunity. Nature 2022;605(7908):139-145 View Article PubMed/NCBI
  72. Pottier C, Wheatherspoon A, Roncarati P, Longuespée R, Herfs M, Duray A, et al. The importance of the tumor microenvironment in the therapeutic management of cancer. Expert Rev Anticancer Ther 2015;15(8):943-954 View Article PubMed/NCBI
  73. Angell H, Galon J. From the immune contexture to the Immunoscore: the role of prognostic and predictive immune markers in cancer. Curr Opin Immunol 2013;25(2):261-267 View Article PubMed/NCBI
  74. Wang T, Niu G, Kortylewski M, Burdelya L, Shain K, Zhang S, et al. Regulation of the innate and adaptive immune responses by Stat-3 signaling in tumor cells. Nat Med 2004;10(1):48-54 View Article PubMed/NCBI
  75. Bruno TC, Ebner PJ, Moore BL, Squalls OG, Waugh KA, Eruslanov EB, et al. Antigen-Presenting Intratumoral B Cells Affect CD4(+) TIL Phenotypes in Non-Small Cell Lung Cancer Patients. Cancer Immunol Res 2017;5(10):898-907 View Article PubMed/NCBI
  76. Bader JE, Voss K, Rathmell JC. Targeting Metabolism to Improve the Tumor Microenvironment for Cancer Immunotherapy. Mol Cell 2020;78(6):1019-1033 View Article PubMed/NCBI
  77. Wesolowski R, Markowitz J, Carson WE. Myeloid derived suppressor cells - a new therapeutic target in the treatment of cancer. J Immunother Cancer 2013;1:10 View Article PubMed/NCBI
  78. Orimo A, Gupta PB, Sgroi DC, Arenzana-Seisdedos F, Delaunay T, Naeem R, et al. Stromal fibroblasts present in invasive human breast carcinomas promote tumor growth and angiogenesis through elevated SDF-1/CXCL12 secretion. Cell 2005;121(3):335-348 View Article PubMed/NCBI
  79. Xue R, Zhang Q, Cao Q, Kong R, Xiang X, Liu H, et al. Liver tumour immune microenvironment subtypes and neutrophil heterogeneity. Nature 2022;612(7938):141-147 View Article PubMed/NCBI
  80. Kaufman HL. Precision immunology: the promise of immunotherapy for the treatment of cancer. J Clin Oncol 2015;33(12):1315-1317 View Article PubMed/NCBI
  81. Woo SR, Corrales L, Gajewski TF. The STING pathway and the T cell-inflamed tumor microenvironment. Trends Immunol 2015;36(4):250-256 View Article PubMed/NCBI
  82. T G S. Innate and adaptive immune cells in Tumor microenvironment. Gulf J Oncolog 2021;1(35):77-81 PubMed/NCBI
  83. Woo SR, Turnis ME, Goldberg MV, Bankoti J, Selby M, Nirschl CJ, et al. Immune inhibitory molecules LAG-3 and PD-1 synergistically regulate T-cell function to promote tumoral immune escape. Cancer Res 2012;72(4):917-927 View Article PubMed/NCBI
  84. Smyth MJ, Ngiow SF, Ribas A, Teng MW. Combination cancer immunotherapies tailored to the tumour microenvironment. Nat Rev Clin Oncol 2016;13(3):143-158 View Article PubMed/NCBI
  85. Rodrigo JP, Sánchez-Canteli M, López F, Wolf GT, Hernández-Prera JC, Williams MD, et al. Tumor-Infiltrating Lymphocytes in the Tumor Microenvironment of Laryngeal Squamous Cell Carcinoma: Systematic Review and Meta-Analysis. Biomedicines 2021;9(5):486 View Article PubMed/NCBI
  86. Fanale D, Dimino A, Pedone E, Brando C, Corsini LR, Filorizzo C, et al. Prognostic and Predictive Role of Tumor-Infiltrating Lymphocytes (TILs) in Ovarian Cancer. Cancers (Basel) 2022;14(18):4344 View Article PubMed/NCBI
  87. Laumont CM, Banville AC, Gilardi M, Hollern DP, Nelson BH. Tumour-infiltrating B cells: immunological mechanisms, clinical impact and therapeutic opportunities. Nat Rev Cancer 2022;22(7):414-430 View Article PubMed/NCBI
  88. Ribas A, Medina T, Kummar S, Amin A, Kalbasi A, Drabick JJ, et al. SD-101 in Combination with Pembrolizumab in Advanced Melanoma: Results of a Phase Ib, Multicenter Study. Cancer Discov 2018;8(10):1250-1257 View Article PubMed/NCBI
  89. Li X, Liu Z, Zhang A, Han C, Shen A, Jiang L, et al. NQO1 targeting prodrug triggers innate sensing to overcome checkpoint blockade resistance. Nat Commun 2019;10(1):3251 View Article PubMed/NCBI
  90. Hu J, Zhang L, Xia H, Yan Y, Zhu X, Sun F, et al. Tumor microenvironment remodeling after neoadjuvant immunotherapy in non-small cell lung cancer revealed by single-cell RNA sequencing. Genome Med 2023;15(1):14 View Article PubMed/NCBI
Copyright © 2024 Authors. This is an Open Access article distributed under the terms of the Creative Commons Attribution-Noncommercial 4.0 License (CC BY-NC 4.0), permitting all non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.

About this Article

Cite this article
Sun Z, Qiu L, Zhao X, Jiao S. The Tumor Microenvironment and Tumor-infiltrating Lymphocytes in Solid Tumor: A Comprehensive Review. Oncol Adv. 2024;2(2):72-82. doi: 10.14218/OnA.2023.00043.
Copy        Export to RIS        Export to EndNote
Article History
Received Revised Accepted Published
November 29, 2023 February 4, 2024 May 22, 2024 June 25, 2024
DOI http://dx.doi.org/10.14218/OnA.2023.00043
  • Oncology Advances
  • eISSN 2996-3427
  • © 2025 Xia & He Publishing Inc.
  • Total visits : 2824157
  • Visits today : 2220
Back to Top

The Tumor Microenvironment and Tumor-infiltrating Lymphocytes in Solid Tumor: A Comprehensive Review

Zhuoya Sun, Lupeng Qiu, Xiao Zhao, Shunchang Jiao
  • Reset Zoom
  • Download TIFF