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Network pharmacology-based analysis of the mechanism of Radix Paeoniae Alba against Toosendan Fructus-induced hepatotoxicity

  • Heng Xu1,
  • Yaqi Zhang2,
  • Huan Chen2 and
  • Tengfei Bai2
 Author information 

Abstract

Objective

To analyze and explore the key targets and molecular mechanisms of action of Radix Paeoniae Alba against Toosendan Fructus-induced hepatotoxicity and the relationship between corresponding compounds based on network pharmacology.

Methods

Using network pharmacology, a "traditional Chinese medicine–chemical composition–key target–pathway" analysis was conducted on Radix Paeoniae Alba for the treatment of Toosendan Fructus-induced hepatotoxicity. The possible mechanism of action was analyzed in terms of function.

Results

The core targets, such as interleukin (IL)-6, tumor necrosis factor (TNF), heat shock protein 90 alpha family class A member 1 (HSP90AA1), peroxisome proliferator-activated receptor gamma (PPARG), prostaglandin-endoperoxide synthase 2 (PTGS2), heme oxygenase 1 (HMOX1), Jun proto-oncogene (JUN), caspase-3, estrogen receptor 1 (ESR1), and aryl hydrocarbon receptor (AHR) were screened from the targets of Radix Paeoniae Alba against Toosendan Fructus-induced hepatotoxicity. Biological process (BP) of toxic targets (BP terms) involved "response to drug; activation of cysteine-type endopeptidase activity involved in apoptotic process," positive regulation of transcription. Cellular components (CC terms) mainly involved cytosol and membrane rafts. Molecular function (MF) terms included "protein homodimerization activity," RNA polymerase II transcription factor activity and enzyme binding, etc." The Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway included the TNF signaling pathway, cancer pathways, and apoptosis.

Conclusion

Radix Paeoniae Alba might alleviate Toosendan Fructus-induced hepatotoxicity through IL6, TNF, HSP90AA1, PPARG, PTGS2, HMOX1, and other targets, possibly via the activation of cysteine-type endopeptidase activity involved in these pathways.

Keywords

Radix Paeoniae Alba, Toosendan Fructus, liver toxicity, network pharmacology, compatibility

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Cite this article
Xu H, Zhang Y, Chen H, Bai T. Network pharmacology-based analysis of the mechanism of Radix Paeoniae Alba against Toosendan Fructus-induced hepatotoxicity. Gastroenterol & Hepatol Res. 2022;4(1):3. doi: 10.53388/ghr2022-03-045.
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Article History
Received Revised Accepted Published
March 30, 2022
DOI http://dx.doi.org/10.53388/ghr2022-03-045
  • Gastroenterology & Hepatology Research
  • eISSN 2703-173X
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Network pharmacology-based analysis of the mechanism of Radix Paeoniae Alba against Toosendan Fructus-induced hepatotoxicity

Heng Xu, Yaqi Zhang, Huan Chen, Tengfei Bai
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