- Article
- OPEN ACCESS
-
Effect of Hirudin on farnesol X receptor pathway during acute intrahepatic cholestasis
-
Yu-Qing Liu1,
-
Yao Wang2,
-
Wen-Qian Tang1,
-
Xin Cai1,
-
Ren-Wu Qin3,
-
Lei Luo4 and
-
Fan Yang5,*
Author information
School of First Clinical Medicine, Hubei University of Chinese Medicine, Hubei Wuhan 430061, China
Department of Infectious Diseases, Renmin Hospital of Wuhan University, Hubei Wuhan 430060, China
Yichang Hospital of TCM Affiliated to Clinical Medical College of TCM of China Three Gorges University, Hubei Yichang 443003, China
The Second People's Hospital of China Three Gorges University, Hubei Yichang 443000, China
Department of Hepatology, Hubei Provincial Hospital of Chinese Medicine, Hubei Wuhan 430061, China
Correspondence to: Fan Yang, Department of Hepatology, Hubei Provincial Hospital of Chinese Medicine, Hubei Wuhan 430061, China. E-mail:
yfzz99@163.com
Abstract
Objective
The purpose of this study is to explore the effect of Hirudin on the farnesoid X receptor (FXR) pathway during acute intrahepatic cholestasis in vivo and in vitro.
Method
In vivo, sixty male Sprague-Dawley rats were randomly divided into six groups: regular group, model group, ursodeoxycholic acid (UDCA) group (60 mg/kg), hirudin treatment group (84 u/kg), hirudin treatment group (63 u/kg) and hirudin treatment group (42 u/kg).The male Sprague-Dawley rats of UDCA group were intragastrically administered with a corresponding concentration of 0.005 mL/g body weight for seven days, once a day; and the hirudin treatment group was injected subcutaneously with different concentrations of Hirudin for seven days, once a day; Except for the normal group, other groups of rats were given 100 mg/kg ANIT by gavage on the 5th day. The model was administered by gavage once a day for three days. In vitro, (Z)-Guggulsterone was used to stimulate the L02 cells (0.05 μmol/ml), with or without different concentrations of Hirudin (2, 4 and 8 u/ml) for 24 h. The liver tissue was examined by HE microscope and the pathological state of the rat liver was observed; FXR, Small heterodimeric chaperone receptor (SHP), uridine diphosphate glucuronide transfer 2B4 (UGT2B4), bile salt output pump (BSEP)mRNA and protein expressions were tested by real-time fluorescent quantitative PCR and Western blot test. And immunohistochemistry (IHC) was used to analyze the expression of FXR.
Results
Compared with the model group, the hirudin group can improve liver tissue damage, and promote FXR, SHP, BSEP and UGT2B4 proteins and mRNA expression in vivo and in vitro.
Conclusion
Hirudin can alleviate intrahepatic cholestasis, reduce liver tissue damage. Hirudin can up-regulate the expression of FXR gene, promote the up-regulation of SHP, BSEP and UGT2B4 genes, and inhibit the cholestasis pathway to protect liver cells. The study may provide an effective drug for clinical treatment of intrahepatic cholestasis.
Keywords
hirudin,
(Z)-Guggulsterone,
α-isothiocyanate (ANIT),
cholestasis,
FXR
|
Copyright © 2025 Authors.
This is an Open Access article distributed under the terms of the Creative Commons Attribution-Noncommercial 4.0 License (CC BY-NC 4.0), permitting all non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.
|
About this Article
Cite this article
|
Liu YQ, Wang Y, Tang WQ, Cai X, Qin RW, Luo L, et al. Effect of Hirudin on farnesol X receptor pathway during acute intrahepatic cholestasis. Gastroenterol & Hepatol Res. 2022;4(2):10. doi: 10.53388/ghr2022-06-052.
|
| Copied to clipboard
|
Copy
Export to RIS
Export to EndNote
|
|
Citation copied!
|
Article History
| Received |
Revised |
Accepted |
Published |
|
|
|
|
June 30, 2022
|
DOI
http://dx.doi.org/10.53388/ghr2022-06-052
-
Gastroenterology & Hepatology Research
-
-
eISSN 2703-173X